著者
KANOU Yasuhiko ABE Naoki ISHIDA Junji FUKAMIZU Akiyoshi SEO Hisao MURATA Yoshiharu
出版者
Research Institute of Environmental Medicine, Nagoya University
雑誌
Environmental medicine : annual report of the Research Institute of Environmental Medicine, Nagoya University (ISSN:02870517)
巻号頁・発行日
vol.46, no.1/2, pp.55-57, 2002-12 (Released:2006-01-05)

ZAKI-4 inhibits the activity of calcineurin, a Ca^<2+>-dependent protein phosphatase. From ZAKI-4 gene, two isoforms, a and P are generated by an alternative splicing. In adult mice ZAKI-4 α mRNA was mainly expressed in brain whereas ZAKI-4 β mRNA wasubiquitously. To elucidate the specific function of ZAKI-4 isoforms, we plan to establish ZAKI-4 β knock out mice by homologousrecombination. For this purpose, mouse embryonic stem cells were electroporated with a targeting vector in which ZAKI-4 β sequencewas disrupted by cDNA coding neomycin resistance. Six independent clones out of 466 antibiotics-resistant colonies underwenthomologous recombination at the ZAKI-4 β locus. These clones will be used to establish the knock out mice.

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外部データベース (DOI)

Yahoo!知恵袋 (1 users, 1 posts)

>目的の遺伝子を特定するのでしょうか? ホモロガスリコンビネーションという方法で細胞が特定します。 >何か特別なことをしなければならないのでしょうか? はい。 ホモロジーアームをターゲティングコンストラクトに付ける必要があります。 -------------- 補足 http://ir.nul.nagoya-u.ac.jp/dspace/bitstream/2237/2783/ ...

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