著者

KANOU Yasuhiko ABE Naoki ISHIDA Junji FUKAMIZU Akiyoshi SEO Hisao MURATA Yoshiharu

出版者

Research Institute of Environmental Medicine, Nagoya University

雑誌

Environmental medicine : annual report of the Research Institute of Environmental Medicine, Nagoya University (ISSN:02870517)

巻号頁・発行日

vol.46, no.1/2, pp.55-57, 2002-12 (Released:2006-01-05)

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ZAKI-4 inhibits the activity of calcineurin, a Ca^<2+>-dependent protein phosphatase. From ZAKI-4 gene, two isoforms, a and P are generated by an alternative splicing. In adult mice ZAKI-4 α mRNA was mainly expressed in brain whereas ZAKI-4 β mRNA wasubiquitously. To elucidate the specific function of ZAKI-4 isoforms, we plan to establish ZAKI-4 β knock out mice by homologousrecombination. For this purpose, mouse embryonic stem cells were electroporated with a targeting vector in which ZAKI-4 β sequencewas disrupted by cDNA coding neomycin resistance. Six independent clones out of 466 antibiotics-resistant colonies underwenthomologous recombination at the ZAKI-4 β locus. These clones will be used to establish the knock out mice.