著者
TOHRU TSUKAHARA
出版者
Center For Academic Publications Japan
雑誌
Japanese Journal of Microbiology (ISSN:00215139)
巻号頁・発行日
vol.5, no.4, pp.383-394, 1961 (Released:2008-04-18)
参考文献数
7
被引用文献数
4

The anti-fungal property of caprylic acid on C. albicans, particularly its fungicidal activity, has been studied by colony count using an ordinary plating method and a modification of an electrophoretic method.Caprylic acid exhibits the most remarkable fungistatic and fungicidal properties of all the normal saturated fatty acids with even numbered carbon atoms studied. The fungicidal activity of caprylic acid depends upon the concentration of the acid, the period of contact and the pH of the media.The fungicidal action of caprylic acid is considerably reduced by the simultaneous addition of several agents such as serum, carbohydrate, and polyhydric alcohol. This protective effect, however, disappears after the contact with the acid for 3 hr. Furthermore, when the cells of the organism are once exposed to caprylic acid, its fungicidal activity is not affected by washing them with distilled water. These quantitative and biochemical facts suggest that the adsorption of caprylic acid to the yeast cells must be extremely strong and specific.
著者
Dhillon T.S. Dhillon Elvera K.S.
出版者
財団法人 学会誌刊行センター
雑誌
Japanese Journal of Microbiology (ISSN:00215139)
巻号頁・発行日
vol.20, no.5, pp.385-396, 1976
被引用文献数
20

Wild type phage HK022 was mutagenized by N-methyl-N'-nitro-N-nitrosoguanidine to induce clear plaque mutants. A total of 225 clear plaque mutants were isolated and 198 of these were as-signable to one or the other of the two complementation groups of the corresponding cistrons which have been designated as cI and cII, respectively. Approximately 25% of the c mutants were found to be temperature-sensitive (cts); producing turbid plaques at 32 C and clear plaques at 38 C and above. From complementation tests involving cI and cII mutants, bacteria lysogenic for cII prophage were fre-quently obtained. Double lysogens harboring a cI and a cII prophage were infrequently found and single lysogens harboring only a cI prophage have not been recovered. Bacterial lysogens harboring a prophage carrying a cts mutation in the cI cistron were readily obtainable. However, such lysogens show a lethal phenotype at 40 C and above, although they appear to be fully viable at 32 C. It is shown that by incubation of lysogens harboring a cts mutant of the cI cistron at 42 C, it is possible to isolate cryptic lysogens which are non-immune but harbor at least one of the phage sus+ alleles. Genetic data involving cI, cII, and two complementing sus mutants of essential genes are presented. From these data the following vegetative map is deduced: sus4-cII-cI-sus3.