著者

本間 清一 大町 睦子 田村 敦子 イシャク エリ 藤巻 正生

出版者

財団法人 学会誌刊行センター

雑誌

Journal of Nutritional Science and Vitaminology (ISSN:03014800)

巻号頁・発行日

vol.29, no.3, pp.375-380, 1983

被引用文献数

2 7

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The lipids were extracted from the winged bean (<i>Psophocarpus tetragonolobus</i>) seed with water-saturated <i>n</i>-butanol. Lipids were separated into groups by preparative TLC on silica gel G. The amount of each lipid type was determined by analysis of the fatty acid constituents in each lipid type.<br> Glyceride was the major lipid accounting for 89.6% of the total, followed by an unknown lipid 4%, free fatty acid of 2.3%, 1, 3-diglyceride, 1, 2diglyceride and steryl ester as 1% each and finally a polar lipid as 0.2%. The results show that winged bean oil should be suitable for edible purposes. Triglycerides showed a similar profile of fatty acids to those of whole lipid: the major fatty acids were palmitic (10.9%), stearic (4.5%), oleic (37.1%), linoleic (19.0%), eicosenoic (3.6%), behenic (18.5%) and lignoceric (4.2%) acids. Compared to soybean oil, winged bean oil contained long chain fatty acids and a fairly small amount of linolenic acid which is favorable regarding oil stability against autoxidation.

著者

Dhillon T.S. Dhillon Elvera K.S.

出版者

財団法人 学会誌刊行センター

雑誌

Japanese Journal of Microbiology (ISSN:00215139)

巻号頁・発行日

vol.20, no.5, pp.385-396, 1976

被引用文献数

20

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Wild type phage HK022 was mutagenized by N-methyl-N'-nitro-N-nitrosoguanidine to induce clear plaque mutants. A total of 225 clear plaque mutants were isolated and 198 of these were as-signable to one or the other of the two complementation groups of the corresponding cistrons which have been designated as cI and cII, respectively. Approximately 25% of the c mutants were found to be temperature-sensitive (cts); producing turbid plaques at 32 C and clear plaques at 38 C and above. From complementation tests involving cI and cII mutants, bacteria lysogenic for cII prophage were fre-quently obtained. Double lysogens harboring a cI and a cII prophage were infrequently found and single lysogens harboring only a cI prophage have not been recovered. Bacterial lysogens harboring a prophage carrying a cts mutation in the cI cistron were readily obtainable. However, such lysogens show a lethal phenotype at 40 C and above, although they appear to be fully viable at 32 C. It is shown that by incubation of lysogens harboring a cts mutant of the cI cistron at 42 C, it is possible to isolate cryptic lysogens which are non-immune but harbor at least one of the phage sus+ alleles. Genetic data involving cI, cII, and two complementing sus mutants of essential genes are presented. From these data the following vegetative map is deduced: sus4-cII-cI-sus3.

著者

Cui Yan Otsuka Megumi Fujiwara Yoko

出版者

財団法人 学会誌刊行センター

雑誌

Journal of nutritional science and vitaminology (ISSN:03014800)

巻号頁・発行日

vol.47, no.4, pp.316-320, 2001-08-01

参考文献数

29

被引用文献数

3

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A reduction of dehydroerythorbic acid (DERA) to erythorbic acid (ERA) in vitamin C-deficient guinea pigs was evaluated and compared with that of dehydroascorbic acid (DASA). Thirty-six guinea pigs were fed with vitamin C-deficient diets for 18 days. On day 19, the guinea pigs were divided into four groups for the administration of 100 mg of DERA, ERA, ascorbic acid (ASA), or DASA every day. After 12 days of oral administration, the concentration of DERA, ERA, ASA, and DASA in the liver, adrenal, spleen, kidney, and plasma of guinea pigs was determined by HPLC. A recovery from scurvy was measured in terms of weight gain and serum alkaline phosphatase activity. All four groups showed simi-lar recovery, indicating that the oral administration of relatively high concentrations of DERA reversed the effects of scurvy in vitamin C-deficient guinea pigs. In spite of DERA or DASA administration, ERA or ASA was mainly detected in the tissues. The reduction ratios of DEAR and DASA were similar (approximately 80%) in all tissues except spleen. These re-sults suggest that both DASA and DERA are taken up and reduced to ASA or ERA <i>in vivo</i>.

著者

Sato Norifumi Kawakami Hiroshi Idota Tadashi

出版者

財団法人 学会誌刊行センター

雑誌

Journal of nutritional science and vitaminology (ISSN:03014800)

巻号頁・発行日

vol.46, no.4, pp.175-179, 2000-08-01

被引用文献数

1 4

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The effects of nucleotide and nucleoside supplementation on the formation of tight junctions and the expression of microvilli, as indexes of morphological differentiation were studied by using a human colon adenocarcinoma cell line (Caco-2 cells). The forma-tion of tight junctions and the expression of microvilli were evaluated by measuring the transepithelial electrical resistance (TEER) and observing the cell surface under electron mi-croscopic analysis, respectively. To clarify the nutritional significance of human milk nu-cleotides, we used a nucleotide mixture (and a corresponding nucleoside mixture) with a composition similar to that found in human milk. Nucleotides had no effect on TEER, but nucleosides markedly promoted the increase of TEER. When alkaline phosphatase activity in the brush border membrane was enhanced by the addition of triiodothyronine (TIT), nu-cleotides also promoted the increase of TEER. Cytidine and CMP predominant in the mix-ture influenced the increase of TEER materially. Furthermore, an electron microphotograph of the cell surface showed that nucleosides contributed to the expression of microvilli. Thus the results presented in this study suggest that nucleotide and nucleoside supplementation may enhance the morphological differentiation of Caco-2 cells.

著者

KIM Hyangkyoung KIM Jung-Ha IM Jee-Aee

出版者

財団法人 学会誌刊行センター

雑誌

Journal of Nutritional Science and Vitaminology (ISSN:03014800)

巻号頁・発行日

vol.60, no.5, pp.348-356, 2014

被引用文献数

1

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To investigate the effects of Active Hexose Correlated Compound (AHCC) supplementation and the mechanism action of AHCC in patients with alcohol-induced mildly elevated liver enzyme levels, participants were randomly allocated to the placebo, 1 g AHCC, or 3 g AHCC group and took the supplement for 12 wk. Subjects visited the hospital for clinical and biochemical measurements, for examination of adverse events, to return unused supplements, and to obtain their next supplements. Biochemical tests including liver enzymes, a questionnaire survey, and anthropometric measurements were collected at baseline and every 4 wk thereafter. Adherence and adverse events were evaluated. After 12 wk of supplementation, the percentage change in alanine aminotransferase (ALT) level was significantly different between the placebo (4.02±59.07%) and both AHCC groups (1 g AHCC: 223.89±20.59%, 3 g AHCC: 224.09±30.73%) (<i>p</i>=0.04). Serum levels of tumor necrosis factor-α (<i>p</i><0.05) and interleukin-1β (<i>p</i><0.01) were significantly lower, while those of adiponectin were higher in both AHCC groups than in the placebo group (<i>p</i><0.01). AHCC supplementation for 12 wk may improve the levels of liver enzymes and circulating pro-inflammatory and anti-inflammatory cytokines in patients with alcohol-induced liver enzyme elevation with mildly elevated liver enzyme levels.

著者

Sasaki Satoshi Ushio Fusao Amano Keiko MORIHARA Motohiko TODORIKI Toru UEHARA Yoshio TOYOOKA Teruhiko

出版者

財団法人 学会誌刊行センター

雑誌

Journal of nutritional science and vitaminology (ISSN:03014800)

巻号頁・発行日

vol.46, no.6, pp.285-296, 2000-12-01

被引用文献数

31 250

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Although several self-administered dietary assessment questionnaires have been developed for Japanese subjects, they have seldom been validated with objective mea-sures. We validated a recently developed self administered diet history questionnaire (DHQ) with fatty acids in serum phospholipid fractions, alpha- and beta-carotenes and alpha-toco-pherol in serum as a gold standard using 86 university workers (42 men and 44 women, age-range=24-67 y). The age-adjusted Pearson partial correlation coefficients between the intakes of marine origin <i>n</i>-3 polyunsaturated fatty acids (PUPA) (crude values, energy-ad-justed values by residual method, energy density, and fat density) and the serum phospho-lipid concentrations (percentage of total fatty acids) were 0.49, 0.51, 0.52, 0.48, and 0.58, 0.69, 0.66, 0.69 in men and women respectively. The correlation coefficients between in-takes (μg/d) and the corresponding serum concentrations (μmol/L) were 0.43 and 0.40 in men and 0.42 and 0.60 in women for alpha- and beta-carotene respectively. It was -0.23 in men and -0.22 in women for alpha-tocopherol. The intakes of major foods (g/d) of ma-rine origin <i>n</i>-3 PUPA, alpha- and beta-carotenes showed a relatively high level of correlation with the corresponding serum concentrations, whereas the level was generally lower than those observed in the analysis with the nutrient intakes. The results suggest that DHO ranks individual adequately for marine origin n-3 PUPA, alpha- and beta-carotene intakes.