著者

Atsuta Yuji Takahashi Yoshiko

出版者

Company of Biologists Ltd.

雑誌

Development (ISSN:09501991)

巻号頁・発行日

vol.142, no.13, pp.2329-2337, 2015-07-01

被引用文献数

43

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胚成長と連動する管組織形成メカニズムの解明 -臓器不全治療や再生医療に期待-. 京都大学プレスリリース. 2015-07-03.

著者

Yaguchi Shunsuke Yaguchi Junko Zheng Wei Jin Yinhua Angerer Lynne M. Inaba Kazuo

出版者

the Company of Biologists Ltd.

雑誌

Development (ISSN:09501991)

巻号頁・発行日

vol.138, no.19, pp.4233-4243, 2011-10

被引用文献数

36 10

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Partitioning ectoderm precisely into neurogenic and non-neurogenic regions is an essential step for neurogenesis of almost all bilaterian embryos. Although it is widely accepted that antagonism between BMP and its inhibitors primarily sets up the border between these two types of ectoderm, it is unclear how such extracellular, diffusible molecules create a sharp and precise border at the single-cell level. Here, we show that Fez, a zinc finger protein, functions as an intracellular factor attenuating BMP signaling specifically within the neurogenic region at the anterior end of sea urchin embryos, termed the animal plate. When Fez function is blocked, the size of this neurogenic ectoderm becomes smaller than normal. However, this reduction is rescued in Fez morphants simply by blocking BMP2/4 translation, indicating that Fez maintains the size of the animal plate by attenuating BMP2/4 function. Consistent with this, the gradient of BMP activity along the aboral side of the animal plate, as measured by pSmad1/5/8 levels, drops significantly in cells expressing Fez and this steep decline requires Fez function. Our data reveal that this neurogenic ectoderm produces an intrinsic system that attenuates BMP signaling to ensure the establishment of a stable, well-defined neural territory, the animal plate.

著者

Suzuki Kentaro Daniel Bachiller Chen YiPing P. Kamikawa Mami Ogi Hidenao Haraguchi Ryuma Ogino Yukiko Minami Yasuhiro Mishina Yuji Ahn Kyung Crenshaw III E. Bryan Yamada Gen オギ ヒデナオ ハラグチ リュウマ オギノ ユキコ ヤマダ ゲン 尾木 秀直 原口 竜摩 荻野 由紀子 山田 源

出版者

Company of Biologists

雑誌

Development

巻号頁・発行日

vol.130, no.25, pp.6209-6220, 2003-12

被引用文献数

3 111

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Extra-corporal fertilization depends on the formation of copulatory organs: the external genitalia. Coordinated growth and differentiation of the genital tubercle (GT), an embryonic anlage of external genitalia, generates a proximodistally elongated structure suitable for copulation, erection, uresis and ejaculation. Despite recent progress in molecular embryology, few attempts have been made to elucidate the molecular developmental processes of external genitalia formation. Bone morphogenetic protein genes (Bmp genes) and their antagonists were spatiotemporally expressed during GT development. Exogenously applied BMP increased apoptosis of GT and inhibited its outgrowth. It has been shown that the distal urethral epithelium (DUE), distal epithelia marked by the Fgf8 expression, may control the initial GT outgrowth. Exogenously applied BMP4 downregulated the expression of Fgf8 and Wnt5a, concomitant with increased apoptosis and decreased cell proliferation of the GT mesenchyme. Furthermore, noggin mutants and Bmpr1a conditional mutant mice displayed hypoplasia and hyperplasia of the external genitalia respectively. noggin mutant mice exhibited downregulation of Wnt5a and Fgf8 expression with decreased cell proliferation. Consistent with such findings, Wnt5a mutant mice displayed GT agenesis with decreased cell proliferation. By contrast, Bmpr1a mutant mice displayed decreased apoptosis and augmented Fgf8 expression in the DUE associated with GT hyperplasia. These results suggest that some of the Bmp genes could negatively affect proximodistally oriented outgrowth of GT with regulatory functions on cell proliferation and apoptosis. The DUE region can be marked only until 14.0 dpc (days post coitum) in mouse development, while GT outgrowth continues thereafter. Possible signaling crosstalk among the whole distal GT regions were also investigated.