- 著者
- Ogbomida Emmanuel Temiotan Nakayama Shouta M. M. Bortey-Sam Nesta Oroszlany Balazs Tongo Isioma Enuneku Alex Ajeh Ozekeke Ogbeide Ainerua Martins Oshioriamhe Fasipe Iriagbonse Priscillia Ezemonye Lawrence Ikechukwu Mizukawa Hazuki Ikenaka Yoshinori Ishizuka Mayumi Ogbomida Emmanuel Temiotan Nakayama Shouta M. M. Bortey-Sam Nesta Oroszlany Balazs Tongo Isioma Enuneku Alex Ajeh Ozekeke Ogbeide Ainerua Martins Oshioriamhe Fasipe Iriagbonse Priscillia Ezemonye Lawrence Ikechukwu Mizukawa Hazuki Ikenaka Yoshinori Ishizuka Mayumi Ogbomida Emmanuel Temiotan Nakayama Shouta M. M. Bortey-Sam Nesta Oroszlany Balazs Tongo Isioma Enuneku Alex Ajeh Ozekeke Ogbeide Ainerua Martins Oshioriamhe Fasipe Iriagbonse Priscillia Ezemonye Lawrence Ikechukwu Mizukawa Hazuki Ikenaka Yoshinori Ishizuka Mayumi
- 出版者
- Elsevier
- 雑誌
- Ecotoxicology and environmental safety (ISSN:01476513)
- 巻号頁・発行日
- vol.151, pp.92-102, 2018-04-30
- 被引用文献数
- 48
The use of free range animals for monitoring environmental health offers opportunities to detect exposure and assess the toxicological effects of pollutants in terrestrial ecosystems. Potential human health risk of dietary intake of metals and metalloid via consumption of offal and muscle of free range chicken, cattle and goats by the urban population in Benin City was evaluated. Muscle, gizzard, liver and kidney samples were analyzed for Cr, Mn, Fe, Co, Ni, Cu, Zn, As, Cd, and Pb concentrations using inductively coupled plasma mass spectrometer (ICP-MS) while Hg was determined using Hg analyzer. Mean concentrations of metals (mg/kg ww) varied significantly depending upon the tissues and animal species. Human health risk estimations for children and adults showed estimated daily intake (EDI) values of tissues below oral reference dose (RfD) threshold for non essential metals Cd, As, Pb and Hg thus strongly indicating no possible health risk via consumption of animal based food. Calculated Hazard quotient (THQ) was less than 1 (< 1) for all the metals analyzed for both adult and children. However, Cd and As had the highest value of THQ suggestive of possible health risk associated with continuous consumption of Cd and As contaminated animal based foods. Hazard Index (HI) for additive effect of metals was higher in chicken liver and gizzard for children and chicken liver for adults. Thus, HI indicated that chicken liver and gizzard may contribute significantly to adult and children dietary exposure to heavy metals. Principal component analysis (PCA) showed a clear species difference in metal accumulation between chickens and the ruminants. This study provides baseline data for future studies and also valuable evidence of anthropogenic impacts necessary to initiate national and international policies for control of heavy metal and metalloid content in food items.
- 著者
- Fredj F. Ben Han J. Irie M. Funamizu N. Ghrabi A. Isoda H.
- 出版者
- Elsevier
- 雑誌
- Ecotoxicology and environmental safety (ISSN:01476513)
- 巻号頁・発行日
- vol.84, pp.54-62, 2012-10
- 被引用文献数
- 6 5
Irrigation with treated wastewater (TWW) is a vital alternative for arid and semi-arid lands but it poses pollution-risk to soil, vegetation and groundwater. Therefore, in the present study, in vitro bioassays were used to evaluate the adverse effects of TWW and irrigated-soil extract sample, on mammalian cells, with respect to heavy metal—Ni, Cd, Pb, Fe, Al-content. The heat shock protein (HSP) 47, E-screen, and transepithelial electrical resistance (TEER) assays served to investigate the stress response of treated-HSP47-transfected Chinese hamster ovary (CHO) cells, the estrogenic activity of the samples in MCF-7 breast cancer cells, and the barrier function (BF) of Caco-2 cells. Furthermore, proteomics analyses were performed to shed light on involved mechanisms and to establish pollution biomarkers. Results showed that the TWW elicited a stress response on HSP cells from 0.1% concentration while soil extract samples exhibited a stress at 1%. TWW induced an estrogenic activity at 10%; up-regulating cell proliferation and tumor-related proteins. Soil extract triggered the enhanced expression of HSP70 family proteins as survival mechanisms against their cytotoxicity toward MCF-7 cells. Moreover, depending on the concentration, 1% of soil extract from 20 cm depth (T20) resulted in a disruption of BF in Caco-2 cells involving cell metabolism, protein synthesis and tumor marker proteins, whereas, 5% of T20 induced the expression of BF-related proteins associated to heat shock, oxidative stress, cell proliferation and glycolytic metabolic pathway. These biological techniques were found to be extremely useful to evaluate the impact of wastewater reuse and to establish specific biomarkers that are common proteins for humans, other mammals and plants. Future studies should focus on exposure quantifications.