著者

TAGUCHI SEIICHI ARAKAWA KUMIKO YOKOYAMA KEIICHI TAKEHANA SHINO TAKAGI HIROSHI MOMOSE HARUO

出版者

The Society for Biotechnology, Japan

雑誌

Journal of Bioscience and Bioengineering (ISSN:13891723)

巻号頁・発行日

vol.94, no.5, pp.478-481, 2002

被引用文献数

1

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Processing and activation of the precursor of an extracellular <i>Streptomyces</i> transglutaminase were achieved by using three <i>Streptomyces</i> proteases (SAM-P20, SAM-P26 and SAM-P45), all of which are widely distributed in <i>Streptomyces</i>. The use of these proteases would allow us to develop a production process for the active form of this enzyme in recombinant bacteria.<br>

著者

KONDOH MAKI HIRAI MITSUYO SHODA MAKOTO

出版者

The Society for Biotechnology, Japan

雑誌

Journal of Bioscience and Bioengineering (ISSN:13891723)

巻号頁・発行日

vol.91, no.2, pp.173-177, 2001

被引用文献数

6

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Bacillus subtilis RB14-C was isolated as a potential biological agent to control the occurrence of various plant diseases. Integrated control of damping-off in tomato plants caused by Rhizoctonia solani was carried out in pots using B. subtilis RB14-C and chemical pesticide, flutolanil. The growth of RB14-C was the same in both the flutolanil-containing and the flutolanil-free media, indicating the resistance of RB14-C to flutolanil. Although the productivity of surfactin decreased to one-third in the flutolanil-containing medium, compared with that in the flutolanil-free medium, the productivity of iturin A which is mainly associated with the suppressive ability of RB14-C against plant pathogens was unaffected. The integration of RB14-C and flutolanil reduced the amount of flutolanil used to one-fourth of that of the signle use of flutolanil, with the same efficacy of reducing disease occurrence.

著者

Minamida Kimiko Shiga Kazuki Sujaya I Nengah SONE TERUO YOKOTA ATSUSHI HARA HIROSHI ASANO KOZO TOMITA FUSAO

出版者

公益社団法人日本生物工学会

雑誌

Journal of bioscience and bioengineering (ISSN:13891723)

巻号頁・発行日

vol.99, no.3, pp.230-236, 2005-03-25

被引用文献数

8 34

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The effects of difructose anhydride III (di-D-fructofuranose-1,2′:2,3′-dianhydride; DFA III) administration (3% DFA III for 4 weeks) on rat intestinal microbiota were examined using denaturing gradient gel electrophoresis (DGGE). According to DGGE profiles, the number of bacteria related to Bacteroides acidofaciens and uncultured bacteria within the Clostridium lituseburense group decreased, while that of bacteria related to Bacteroides vulgatus, Bacteroides uniformis and Ruminococcus productus increased in DFA III-fed rat cecum. In the cecal contents of DFA III-fed rats, a lowering of pH and an increase in short chain fatty acids (SCFAs), especially acetic acid, were observed. The DFA III-assimilating bacterium, Ruminococcus sp. M-1, was isolated from the cecal contents of DFA III-fed rats. The strain had 98% similarity with R. productus ATCC 27340T (L76595), and mainly produced acetic acid. These results confirmed that the bacteria harmful to host health were not increased by DFA III administration. Moreover, DFA III stimulated the growth of Ruminococcus sp. M-1 producing acetic acid, which may alter the intestinal microbiota towards a healthier composition. It is expected that DFA III would be a new candidate as a prebiotic.

著者

Yang Hanshuo Wang Chunting Wang Rui Deng Hongxin Ding Zhenyu Yang Jinliang Lu You Li Jiong Zhang Peng Mao Yongqiu Kan Bing Wei Lin Peng Feng Wei Yuquan

出版者

公益社団法人日本生物工学会

雑誌

Journal of bioscience and bioengineering (ISSN:13891723)

巻号頁・発行日

vol.107, no.6, pp.589-595, 2009-06

被引用文献数

1

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Genes involved in tumorigenesis may be targets for cancer immunotherapy. For many tumor types, molecular signatures do not yet exist. Methods that identify novel, tissue-specific tumor-related genes therefore have potentially important implications for developing cancer immunotherapy. Here, we used cross-reactive serological analysis of recombinant cDNA expression (CR-SEREX) to identify the novel testicular cancer-related gene, rat testicular antigenic protein 2 (RTAP2). We identified two RTAP2 splice variants (RTAP2a and RTAP2b) encoding 191 and 358 amino acid proteins, respectively. RTAP2a and RTAP2b proteins have identical N-termini but different C-terminal regions arising from alternative splicing of exon 4. Bioinformatics and reverse transcription-polymerase chain reaction (RT-PCR) revealed that the RTAP2a transcript was expressed selectively in adult testis and several tumor cell lines, while RTAP2b was ubiquitous. Our results suggest that RTAP2a may contribute to testicular development and tumorigenesis, and may be a candidate gene for cancer immunotherapy.