著者

Norihito Aihara Masaru Yamaguchi Kunihiko Yamada Tomokazu Yoshino Takemi Goseki Kazutaka Kasai

出版者

日本大学松戸歯学部 口腔科学研究所

雑誌

International Journal of Oral-Medical Sciences (ISSN:13479733)

巻号頁・発行日

vol.12, no.1, pp.54-60, 2013 (Released:2013-09-04)

参考文献数

29

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The aim of this studywas to investigate how interleukin (IL)-17 and the receptor activator of nuclear factor kappaB (RANK) ligand (RANKL) contribute to root resorption during orthodontic tooth movement. The first objective was to determine the levels of interleukin-17 (IL-17), soluble RANKL (sRANKL) and osteoprotegerin (OPG) in gingival crevicular fluid (GCF) obtained from a patient with severe root resorption after orthodontic treatment. A second objective was to investigate the effects of IL-17 on the release of sRANKL and OPG from human periodontal ligament (hPDL) cells. Subjects with severe root resorption (>1/3 of original root length) were identified. Control group subjects with no loss of root structure while undergoing orthodontic treatment were also identified. GCF was collected non-invasivelyfrom the mesial and distal sides of each of the four upper incisors using filter paper strips. Eluted GCF was used for analysis by enzyme-linked immunosorbent assay (ELISA). Furthermore, this in vitro studywas performed to examine the effects of IL-17 on RANKL and OPG release from human PDL cells using ELISA. Concentrations of IL-17, sRANKL, sRANKL/OPG ratio in GCF were significantlyhigher in subjects with severe root resorption than in those with no resorption. In the in vitro study,IL-17 increased the release of sRANKL, and decreased that of OPG from hPDL cells in a time- and dose-dependent manner. These results suggest that IL-17 stimulates orthodonticallyinduced inflammatoryroot resorption (OIIRR) via production of RANKL in PDL.

著者

Kento Umeki Yutaka Watanabe Hirohiko Hirano

出版者

日本大学松戸歯学部 口腔科学研究所

雑誌

International Journal of Oral-Medical Sciences (ISSN:13479733)

巻号頁・発行日

vol.15, no.3-4, pp.152-159, 2017-03-10 (Released:2017-03-27)

参考文献数

22

被引用文献数

6

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Maintenance and improvement of masticatory function in nursing care elderly persons (NC) is an important issue, and it is speculated that sarcopenia is related to declining masticatory function. The decrease in skeletal muscle index(SMI), a major diagnostic criterion for sarcopenia, has been reported to be associated with swallowing function in NC.However, the relationship between SMI and masticatory function is unknown. Therefore,we investigated the relationship between masseter muscle thickness(MMT)and SMI, with the aim of examining the specific relationship between decreased masticatory function and sarcopenia in NC. MMT and SMI were measured by ultrasonography and bioelectrical impedance analysis in 275 NC participants in Omori Town, Yokote City, Akita Prefecture in the Tohoku region in Japan. Cognitive functions measured from all participants using questionnaire. Participants were classified into low-MMT or high-MMT group based on the median of each of MMT, and SMI and related items in each gender. In addition, to examine the factors related to MMT, logistic regression analysis was conducted by entering age,sex, SMI, nutrition status, severity of dementia, and other items as explanatory variables and MMT as objective variable. SMI in high-MMT group were significantly higher than low-MMT group(high-MMT: 4.8±1.4 kg/m2, low-MMT: 4.4±1.4 kg/m2, P=0.010).Furthermore, logistic regression analysis indicated that SMI were significantly associated with a MMT(Odds Ratio=0.83, 95% Confidence Interval=0.69-0.99, P=0.049). Our result suggested that the mass of the masseter muscles decreased with NC due to sarcopenia, possibly contributing to a decrease in masticatory function.

著者

Chieko Taguchi Kazumune Arikawa Masanori Saitou Toshikazu Uchiyama Itsuki Watanabe Keisuke Tobita Ryoki Kobayashi Tomoko Ochiai Ikuo Nasu

出版者

日本大学松戸歯学部 口腔科学研究所

雑誌

International Journal of Oral-Medical Sciences (ISSN:13479733)

巻号頁・発行日

vol.13, no.3, pp.102-109, 2015 (Released:2015-06-09)

参考文献数

40

被引用文献数

4

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Lactobacillus crispatus KT-11, which was originally isolated from the feces of healthy infants, has been reported to show multiple immunoregulatory effects. However, there have been no reports about the effect of the Lactobacillus on periodontal disease, a chronic destructive inflammatory disease of the tissues supporting the teeth. We used a model of periodontal disease in which mice were infected with Porphyromonas gingivalis. As we report here, oral ingestion of KT-11 exerted inhibitory effects on alveolar bone resorption in this model, suggesting potential preventive activity of the Lactobacillus in periodontal disease. Mice were given free access to feed containing dead Lb. crispatus KT-11 over 6 weeks, and were then orally infected with P. gingivalis 10 times for 2 weeks from 4 weeks later of Lb. crispatus KT-11 administration. The results showed a marked attenuation of alveolar bone resorption in mice that ingested the Lb. crispatus KT-11-containing feed. Induction of total IgG in plasma and total secretory IgA in saliva was observed. A specific plasma IgG antibody response to P. gingivalis also was induced. Based on the immune response to P. gingivalis infection induced by ingestion of Lb. crispatus KT-11, this study suggests that oral administration of Lb. crispatus KT-11 is effective in preventing chronic periodontitis.

著者

Jotaro Ohmori Tonami Ikuta Noboru Kuboyama Yoshimitsu Abiko

出版者

日本大学松戸歯学部 口腔科学研究所

雑誌

International Journal of Oral-Medical Sciences (ISSN:13479733)

巻号頁・発行日

vol.10, no.4, pp.229-234, 2012 (Released:2012-04-28)

参考文献数

20

被引用文献数

1

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Oral epithelial cells significantly influence host inflammatory responses against Candida albicans. Pro-inflammatory cytokines function as an early innate immune system mediator during C. albicans infection in oral epithelial cells. The presence of yeasts in periodontal pockets is well known and C. albicans is the species most commonly isolated from the oral cavity. To elucidate the molecular mechanisms governing the human gingival epithelial cells to C. albicans infection, human gingival epithelial cells (HGE) were primarily cultured and infected with C. albicans ATCC90029. Total RNA was extracted from HGE after 8 hrs of infection and monitored mRNA levels using Affymetrix GeneChip (Human Genome U133 plus 2.0 Array, 48,000 genes). GeneChip data was analyzed by GeneSpring software. The mesenchymal-epithelial transition factor (c-Met), showed a greater than 2-fold change in expression relative to those in control cells. In contrast, hepatocyte growth factor (HGF) showed an absent of gene expression in HGF. Altered mRNA levels of c-Met in GeneChip analysis were confirmed by reverse transcription polymerase chain reaction (RT-PCR) and real-time RT-PCR. Stronger immunoreactivity against c-Met was observed in the infection with C. albicans by using an in vivo rat animal model. These findings suggest that the enhanced c-Met expression is involved in the growth of gingival epithelial cells in response to C. albicans.