- 著者
- Naofumi Tsuchizaki Masa Hamada Kunimoto Hotta
- 出版者
- 日本放線菌学会
- 雑誌
- 日本放線菌学会誌 (ISSN:09145818)
- 巻号頁・発行日
- vol.15, no.2, pp.23-29, 2001-12-25 (Released:2008-03-04)
- 参考文献数
- 26
- 被引用文献数
- 2 3
(Using colony direct PCR we established, kan gene (GenBank accession No. AB028210) coding for an aminoglycoside acetyltransferase, AAC(3), was surveyed among strains of Streptomyces griseus and its related taxa (S. anulatus group). The regulatory and ORF regions of kan gene were targeted by the use of original primers. With the regulatory region primers, the target fragment (518 bp) amplification was observed in all the streptomycin (SM)-producing strains of S. griseus and in several strains of S. anulatus group. The other target fragment (930 bp) amplification using the ORF primers was observed only in the SM-producing strains of S. griseus including ‘S. ornatus’ that has substantially identical properties to those of S. griseus. It was thus conclusive that kan gene is highly conserved in SM-producing strains of S. griseus and distributed in taxa related to S. griseus. There were numbers of strains that provided no amplification upon PCR but hybridization bands upon Southern hybridization, suggesting the lack or alteration of kan gene. Characterization by colony direct PCR using appropriate primers will be a big help for taxonomic as well as ecological characterization of actinomycetes.
- 著者
- Masayuki Hayakawa Hideki Yamamura Youji Nakagawa Yoshika Kawa Yukikazu Hayashi Taku Misonou Hidehiro Kaneko Noriko Kikushima Terumi Takahashi Shuhei Yamasaki Yoshinobu Uegaki Akihiro Terasawa Kiyotoshi Takao Tomokazu Mori Ken-ichi Ago Nanako Saito Tatsuya Hasegawa
- 出版者
- 日本放線菌学会
- 雑誌
- 日本放線菌学会誌 (ISSN:09145818)
- 巻号頁・発行日
- vol.24, no.2, pp.58-62, 2010-12-25 (Released:2010-12-15)
- 参考文献数
- 28
- 被引用文献数
- 4 8 5
In this study, we investigated the taxonomic diversity of actinomycetes isolated from swine manure compost that had undergone self-heating. Pretreatment with sodium dodecyl sulfate and subsequent cultivation of the compost sample on humic acid-vitamin agar at 30 and 50°C resulted in the selective isolation of 125 actinomycete strains. These isolates were classified using a combination of morphological characterization and 16S rDNA sequence analysis, with sequence similarities of ≥97% and ≥99% used to identify isolates at genus and species level, respectively. The isolated actinomycete assemblage consisted of a diverse array of species, with the 125 isolates falling into 10 families, 15 genera, and 22 species. The most dominant thermophilic actinomycetes were Saccharomonospora (Sam.) viridis and Thermobifida fusca, followed by Actinomadura spp. Other species that were frequently isolated at 30°C included Glycomyces spp., Nocardiopsis (Nop.) alba, Nop. composta, Rhodococcus rhodochrous, and Sam. azurea.