著者

Gobi K. Vengatajalabathy Mizutani Fumio

出版者

社団法人 日本分析化学会

雑誌

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry (ISSN:09106340)

巻号頁・発行日

vol.17, no.1, pp.11-15, 2001-01-10

参考文献数

31

被引用文献数

1 6

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Amperometric measurement of superoxide dismutase (SOD) was carried out at cytochrome <i>c</i>-immobilized monolayers and ascorbate oxidase (AOD)/xanthine oxidase (XOD)/cytochrome <i>c</i>- and (AOD, XOD)/cytochrome <i>c</i>-multilayers. Cytochrome <i>c</i> was covalently immobilized on mercaptopropionic acid-containing self-assembled monolayers on gold. A biopolymer membrane of poly-L-lysine confining XOD and AOD was cast on the monolayer of cytochrome <i>c</i>. While both the cytochrome <i>c</i>-immobilized monolayer and multilayer electrodes show anodic current responses to the generation of superoxide radical, the sensitivity of the multilayer system for the detection of superoxide radical was high relative to that of the monolayer system. In the case of the cytochrome <i>c</i>-multilayer electrodes, the generation of superoxide radical near the sensing element, cytochrome <i>c</i>, resulted in high sensitivity for the detection of superoxide. The use of a XOD and AOD-incorporated poly-L-lysine membrane enabled the detection of the generation of superoxide radical in the presence of L-ascorbic acid. Though L-ascorbic acid could scavenge superoxide radical, the biopolymer membrane confined with AOD will oxidize any L-ascorbic acid that permeated into the membrane. By using the multilayer electrodes, one could measure the activity of SOD in the presence of L-ascorbic acid.

著者

Ruiz-Bevia Francisco Fernandez-Torres Maria J.

出版者

社団法人 日本分析化学会

雑誌

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry (ISSN:09106340)

巻号頁・発行日

vol.26, no.6, pp.723-726, 2010-06-10

参考文献数

19

被引用文献数

6

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The influence of seawater salts as salting out agents on the purge-and-trap gas chromatography (PT-GC) determination of trihalomethanes (THMs) was studied. This is particularly important since seawater is chlorinated when used as a cooling agent in coastal nuclear power stations. The chlorination produces unwanted THMs as by-products. A PT-GC apparatus was used to determine the Henry&rsquo;s Law constant of each THM, with seawater as the sample matrix.

著者

CAO Xueling LIAN Lili LI Hongwei WU Yuqing LOU Dawei

出版者

社団法人 日本分析化学会

雑誌

Analytical Sciences (ISSN:09106340)

巻号頁・発行日

vol.30, no.8, pp.817-822, 2014

被引用文献数

6

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In the present study, biomolecule-stabilized Au nanoclusters were demonstrated as a novel fluorescence probe for sensitive and selective detection of pazufloxacin mesilate (PZFX) for the first time. The linear decrease in the fluorescence intensity of Au nanoclusters induced by PZFX allowed for the quantitative detection of PZFX in the range of 0.15 &mu;g/mL to 1 mg/mL, and the detection limit for PZFX was 0.2 &mu;g/mL. Circular dichroism spectroscopy and fluorescence decay studies were then performed to discuss the quenching mechanism. In addition, practical application of the present approach was also demonstrated for real samples, which suggested its great potential for accurate analysis of similar drugs.

著者

ZHANG Hao GUAN Yanan LI Xiaoshuang LIAN Lili WANG Xiyue GAO Wenxiu ZHU Bo LIU Xuying LOU Dawei

出版者

社団法人 日本分析化学会

雑誌

Analytical Sciences (ISSN:09106340)

巻号頁・発行日

vol.34, no.10, pp.1155-1161, 2018

被引用文献数

13

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<p>This paper describes a novel method for label-free mercury(II) ion detection based on exonuclease III-induced target signal recycling amplification using double-stranded DNA templated copper nanoclusters. The synthesized DNA-Cu nanoclusters were used with exonuclease III loop amplification technology for ultra-high sensitivity detection of mercury(II) ions, which were detected by significantly decreased fluorescence intensity. Under the optimal experimental conditions, there was a clear linear relationship between Hg²⁺ concentration in the range of 0.04 to 8 nM and fluorescence intensity. The detection limit for Hg²⁺ was 4 pM. In addition, the interference of other metal ions on the mercury(II) ion detection was also studied. To confirm the application of the fluorescent sensor, it was applied to determine the concentrations of mercury(II) ions in tap water, and the results showed that the method can be used to detect mercury(II) ions in water samples successfully.</p>

著者

JIANG Zhiliang WEI Lili LIANG Aihui

出版者

社団法人 日本分析化学会

雑誌

Analytical Sciences (ISSN:09106340)

巻号頁・発行日

vol.25, no.4, pp.463-467, 2009

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Nanogold particles of size 15 nm were used to label goat anti-human ceruloplasmin (GCP) to obtain an immunonanogold probe (AuGCP) for ceruloplasmin (CP). In a pH 7.8 citric acid-Na₂HPO₄ buffer solution and in the presence of polyethylene glycol (PEG), an immunoreaction between AuGCP and CP took place, and the released nanogold particles aggregated to bigger clusters, which caused the resonance scattering (RS) intensity at 552 nm (<i>I</i>_552nm) to be enhanced greatly. The enhanced intensity &Delta;<i>I</i> was proportional to the CP concentration (<i>C</i>_CP) in the range from 0.0030 to 1.26 &micro;g/mL, with a regress equation of &Delta;<i>I</i> = 101.0<i>C</i>_CP + 1.8, a correlation coefficient of 0.9970, and a detection limit of 1.1 ng/mL CP. This simple and sensitive RS immunoassay was applied to the determination of CP in human plasma, with satisfactory results.

著者

Atsuko MASUYA-SUZUKI Nobuhiko IKI

出版者

社団法人 日本分析化学会

雑誌

X-ray Structure Analysis Online (ISSN:18833578)

巻号頁・発行日

vol.32, pp.41-43, 2016-09-10 (Released:2016-09-10)

参考文献数

8

被引用文献数

1