著者

Kohanbash Gary Ishikawa Eiichi Fujita Mitsugu Ikeura Maki McKaveney Kayla Zhu Jianzhong Sakaki Masashi Sarkar Saumendra N. Okada Hideho

出版者

Landes Bioscience

雑誌

OncoImmunology (ISSN:21624011)

巻号頁・発行日

vol.1, no.4, pp.487-492, 2012-07

被引用文献数

10 5

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We have previously reported that the single nucleotide polymorphism (SNP) rs12553612 in IFNA8 is associated with better overall survival of glioma patients with the AA-genotype compared with patients with the AC-genotype. As rs12553612 is located in the IFNA8 promoter, we hypothesized that the A-allele allows for an enhanced IFNA8 promoter activity compared with the C-allele. Reporter assays in the human monocyte derived THP-1 cell line demonstrated a superior promoter activity of the A-allele compared with the C-allele. Electrophoretic mobility shift assays (EMSA) further demonstrated that the A-genotype specifically binds to more nuclear proteins than the C-genotype, including the transcription factor Oct-1. Further, co-transfection of plasmids encoding Oct-1 and the reporter constructs revealed that Oct-1 enhanced the promoter activity with the A- but not the C-allele. Taken together, our data demonstrate that the A-allele in the rs12553612 SNP, which is associated with better glioma patient survival, allows for IFNA8 transcription by allowing for Oct-1 binding, which is absent in patients with C allele, and suggests a molecular mechanism of IFNA8 mediated immune-surveillance of glioma progression.

著者

Destici Eugin Oklejewicz Małgorzata Saito Shoko van der Horst Gijsbertus T.J.

出版者

Landes Bioscience

雑誌

Cell cycle (ISSN:15384101)

巻号頁・発行日

vol.10, no.21, pp.3788-3797, 2011-11

被引用文献数

21 8

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By gating cell cycle progression to specific times of the day, the intracellular circadian clock is thought to reduce the exposure of replicating cells to potentially hazardous environmental and endogenous genotoxic compounds. Although core clock gene defects that eradicate circadian rhythmicity can cause an altered in vivo genotoxic stress response and aberrant proliferation rate, it remains to be determined to what extent these cell cycle related phenotypes are due to a cell-autonomous lack of circadian oscillations. We investigated the DNA damage sensitivity and proliferative capacity of cultured primary Cry1‑/-

出版者

Landes Bioscience

巻号頁・発行日

0000