To identify novel cross-domain symbiosis between Candidatus Patescibacteria and Archaea, we performed fluorescence in situ hybridization (FISH) on enrichment cultures derived from methanogenic bioreactor sludge with the newly designed 32-520-1066 probe targeting the family-level uncultured clade 32-520/UBA5633 lineage in the class Ca. Paceibacteria. All FISH-detectable 32-520/UBA5633 cells were attached to Methanospirillum, indicating high host specificity. Transmission electron microscopy observations revealed 32-520/UBA5633-like cells that were specifically adherent to the plug structure of Methanospirillum-like rod-shaped cells. The metagenome-assembled genomes of 32-520/UBA5633 encoded unique gene clusters comprising pilin signal peptides and type IV pilins. These results provide novel insights into unseen symbiosis between Ca. Patescibacteria and Archaea.
» Novel Cross-domain Symbiosis between <i>Candidatus</i> Patescibacteria and Hydrogenotrophic Methanogenic Archaea <i>Methanospirillum</i> Discovered in a Methanogenic Ecosystem https://t.co/JJX9cnFowy
J-STAGE Articles - Novel Cross-domain Symbiosis between <i>Candidatus</i> Patescibacteria and Hydrogenotrophic Methanogenic Archaea <i>Methanospirillum</i> Discovered in a Methanogenic Ecosystem https://t.co/xPRsNbU9zA
We discovered novel and second cross-domain symbiosis between Ca. Patescibacteria/CPR and the hydrogenotrophic methanogen Methanospirillum.
https://t.co/gJf7B7hsPL