著者
Yoshinori FUJIYOSHI
出版者
The Japan Academy
雑誌
Proceedings of the Japan Academy, Series B (ISSN:03862208)
巻号頁・発行日
vol.91, no.9, pp.447-468, 2015-11-11 (Released:2015-11-11)
参考文献数
49
被引用文献数
1 2

Electron crystallography is especially useful for studying the structure and function of membrane proteins — key molecules with important functions in neural and other cells. Electron crystallography is now an established technique for analyzing the structures of membrane proteins in lipid bilayers that closely simulate their natural biological environment. Utilizing cryo-electron microscopes with helium-cooled specimen stages that were developed through a personal motivation to understand the functions of neural systems from a structural point of view, the structures of membrane proteins can be analyzed at a higher than 3 Å resolution. This review covers four objectives. First, I introduce the new research field of structural physiology. Second, I recount some of the struggles involved in developing cryo-electron microscopes. Third, I review the structural and functional analyses of membrane proteins mainly by electron crystallography using cryo-electron microscopes. Finally, I discuss multifunctional channels named “adhennels” based on structures analyzed using electron and X-ray crystallography.

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外部データベース (DOI)

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@Masamichi8787 I think this is the only source available so far, but hopefully should be published soon. https://t.co/KkYxzcl6tj
国内誌?に寄せた文章に"We are currently developing a new method, named Image Based Structure Analysis (IBSA), together with a… https://t.co/xnFuF6fTbZ

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