著者
徐 可樹 竹上 勉
出版者
Japanese Society of Tropical Medicine
雑誌
Japanese Journal of Tropical Medicine and Hygiene (ISSN:03042146)
巻号頁・発行日
vol.24, no.2, pp.99-105, 1996-06-15 (Released:2011-05-20)
参考文献数
21
被引用文献数
1 2

Suramin, a polysulfonated naphthylurea, has been used for the treatment of trypanosomias and onchocerciasis, and is a potent inhibitor of nucleic acid polymerase including reverse transcriptase and DNA polymerase. This drug is used to examine an antiviral activity in the case of Japanese encephalitis virus (JEV) infection. Japanese encephalitis occurs in endemic and epidemic form over a wide area of Asia, at least tens of thousands of cases occur annually in East Asia. Although the vaccine against JEV is widely used, we have no antiviral drugs against JEV replication. Here we describe an antiviral activity of suramin on JEV replication in the cultured cells. In the presence of 50 μg/ml suramin, virus yields in human neuroblastoma cell line, IMR-32, reduced to 0.1% of control level. JEV growth in human hepatoma cell line, HepG2, was also inhibited, but inhibitory effect of suramin was lower than in IMR-32. The difference of inhibitory effects between host cells suggests that some host factors were involved in the process of inhibition of JEV growth. By Western blot analysis, it was clarified that expressions of JEV proteins, NS3 and E were markedly reduced by the treatment of suramin at 50-200 μg/ml. Especially the expression of E protein seems to be sensitive against suramin treatment. On the other hand, JEV-RNA level in the cells treated with suramin was not so different from control level, and in vitro JEV-RNA synthesis was also not inhibited by the addition of suramin. These results suggest that suramin inhibits virus replication through the influence to viral protein production, not to viral RNA synthesis.

言及状況

外部データベース (DOI)

Twitter (52 users, 52 posts, 93 favorites)

スラミンは松葉茶に含まれていない スラミンはバイエル社が作り出した アフリカのトリパノソーマ症の治療薬 レトロウイルス逆転写酵素阻害剤 Eタンパク質の産生阻害 https://t.co/SQfU8bsBm0
@Mii2410yumomo ワク珍の効果  ↓ 酸化グラフェンで血液に影響 寄生虫(トリパノソーマ)で人体へ恐るべき影響(心不全、大腸の破壊、皮膚、脳への影響など) ワクチンに含まれるmRNAの量はごくわずか このことから、虫下しのイベルメクチンと繋がる。 同じくスラミンもいいってことだね。 https://t.co/Zh9y26poUR
マイコヴィッツ博士の言ってたスラミンって薬の話でしょ? 松葉茶でスラミンが摂れるってなんでそんな話が流行ってんの? 松葉茶にスラミン何mgとかどこ見てもひと言も書いてないよな? https://t.co/Dq7w0egkJ7 https://t.co/rFjo2mGWfF
松に含有されるスラミンの日本脳炎ウイルスへの増殖抑制効果   ↓ ↓ ↓ https://t.co/Ul7DMPKNOZ 日本脳炎ウイルスは、一本鎖プラス鎖RNAウイルスで、新型コロナウイルスの遠い親戚。 日本人に新型コロナ感染者が少ない理由の一つに、日本脳炎へのヴァクシーン接種済だから、という説がある。 https://t.co/QCCYUeNgvq

収集済み URL リスト