著者

朴美姫 鈴木 義人 蝶野 真喜子 山口 五十麿

出版者

植物化学調節学会

雑誌

植物化学調節学会研究発表記録集 (ISSN:09191887)

巻号頁・発行日

no.36, pp.43-44, 2001-10-09

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Gibberellin responsive gene 092 was isolated from cucumber hypocotyls by means increased in hypocotyls of cucumber by GA treatment. The sequence data suggested that the clone encoded an arabinogalactan-protein. The ful1-length cDNA of 092 was introduced into tobacco plants under the control of 35S promoter The AGP was extracted and purified from the transgenic tobacco by reversed-phase-high performance liquid chromatography (RP-HPLC) and gel filtration chromatography (GFC) and analyzed Using β-glucosyl Yariv reagent which binds selectively with AGPs. The transgenic plants gave aβ-Yariv-reactive peak in addition to those present in wild type plants. β-Yariv regent inhibited hypocotyl elongation promoted by GA and IAA treannent in intact cucumber seedling and hypocotyl segments of azuki bean. Together with results using anti-AGP antibodies, the product of 092 was suggested to be an AGP. The transgenic tobacco plants showed earlier flowering than wide ty'pe plants.