著者

本多 一郎 和田 道宏 阿部 知子 浅見 忠男 吉田 茂男

出版者

植物化学調節学会

雑誌

植物化学調節学会研究発表記録集 (ISSN:09191887)

巻号頁・発行日

no.35, pp.147-148, 2000-11-02

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To obtain novel mutants of barley (Horderum vulgare L), heavy ion irradiation were conducted. Both imbibed and dry seeds of barley (Kanto Hadaka No.77) were irradiated with 5-400 [gray] of dose by heavy-ion beam (^<14>N; 135MeV/u) and cultivated on field. Severe growth inhibition were observed in over 10 and 200 gray irradiation in imbibed and dry seed irradiation, respectively. After harvesting M_2 seeds, an aliquot of seeds were sown to field, candidates of mutants were serveyed, and selected. After crossing, their F_1 and F_2 plant phenotype were examined, 3 dwarf lines which have ressesive allele were separated. These results suggest that heavy-ion irradiation is effective method to mutate barley.

著者

千葉 康隆 大坂 正明 山口 信次郎 三橋 渉 豊増 知伸

出版者

植物化学調節学会

雑誌

植物化学調節学会研究発表記録集 (ISSN:09191887)

巻号頁・発行日

no.45, 2010-10-01

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Fusicoccins are produced by plant-pathogenic fungus Phomopsis amygdali, and possess phytohormone-related activities. Recently, we identified an unusual chimeric diterpene synthase gene, PaFS, which is responsible for fusicoccin biosynthesis. PaFS encodes fusicoccadiene (biosynthesis intermediate cyclic hydrocarbon of fusicoccin) synthase (719 amino acids), possessing the geranylgeranyl diphosphate (GGDP) cyclase domain at the N-terminus and GGDP synthase (GGS) domain at the C-terminus. In this study, we tried to generate transgenic Arabidopsis thaliana plants expressing PaFS or N390 (N-terminus 390 amino acids of PaFS), possessing only diterpene cyclase activity, in plastids, containing abundant GGDP, or in the cytoplasm to examine wthether the fungal diterpenes are produced efficiently by the chimeric enzyme in plant cells. To target the fungal enzymes to plastids, a transit peptide (TP) of Arabidopsis copalyl diphosphate synthase was fused to their N-terminus. The four cDNA (PaFS, TP-PaFS, N390, TP-N390) were introduced to Arabidopsis through Agrobacterium infection. It was confirmed that each translated product from the transgene had proper activity by in vitro assay using bacterial recombinant protein, and RTPCR anylysis showed that each transcript was detected in T1 plants. We will further characterize each transgenic Arabidopsis using T2 plants.

著者

堅固山 裕子 北畑 信隆 安藤 卓也 浅見 忠男

出版者

植物化学調節学会

雑誌

植物化学調節学会研究発表記録集 (ISSN:09191887)

巻号頁・発行日

vol.48, 2013-10-04

著者

北畑 信隆 早瀬 大貴 Bisson Melanie M. A. 湯本 弘子 中野 雄司 中山 真義 Groth Gerog 浅見 忠男

出版者

植物化学調節学会

雑誌

植物化学調節学会研究発表記録集 (ISSN:09191887)

巻号頁・発行日

no.46, 2011-10-03

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The gaseous hormone ethylene plays important roles in many physiological and developmental processes in plants. To regulate ethylene signaling, we screened novel chemicals with ethylene mimic activity that induce triple response phenotype of etiolated seedlings. Finally we identified a compound with ethylene mimic activity, named HJ2. Ethylene biosynthetic inhibitor did not suppress HJ2-induced phenotype. On the other hand, ethylene insensitive mutant, ein2, suppressed HJ2-induced phenotype. Moreover, antagonist of ethylene receptor, STS, suppressed HJ2-induced phenotype in dose-dependent manner. These results suggested that HJ2 is an agonist of ethylene receptor. To improve ethylene activity of HJ2, we designed and synthesized HJ2 derivatives. As a result, we developed more effective ethylene agonists. At present, we examine binding of these chemicals to ETR1 protein in detail.

著者

蔡 相憲 向田 賢 平田 貴寛 鄭 憲栽 横田 孝雄 米山 弘一 竹内 安智

出版者

植物化学調節学会

雑誌

植物化学調節学会研究発表記録集 (ISSN:09191887)

巻号頁・発行日

no.38, 2003-10-10

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Seed germination of S. hermonthica is induced by stimulants produced by host and non-host plants. The seeds require conditioning in the dark for a certain period before the seeds become responsive to the exogenous germination stimulants. In our experiments, fluridone, a carotenoid biosynthesis inhibitor, shortened the conditioning period and increased the rate of the seed germination induced by GR-24,a germination stimulant. Fluridone was also effective in amelorating the inhibitory effects of light on the conditioning and on the germination.

著者

朴美姫 鈴木 義人 蝶野 真喜子 山口 五十麿

出版者

植物化学調節学会

雑誌

植物化学調節学会研究発表記録集 (ISSN:09191887)

巻号頁・発行日

no.36, pp.43-44, 2001-10-09

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Gibberellin responsive gene 092 was isolated from cucumber hypocotyls by means increased in hypocotyls of cucumber by GA treatment. The sequence data suggested that the clone encoded an arabinogalactan-protein. The ful1-length cDNA of 092 was introduced into tobacco plants under the control of 35S promoter The AGP was extracted and purified from the transgenic tobacco by reversed-phase-high performance liquid chromatography (RP-HPLC) and gel filtration chromatography (GFC) and analyzed Using β-glucosyl Yariv reagent which binds selectively with AGPs. The transgenic plants gave aβ-Yariv-reactive peak in addition to those present in wild type plants. β-Yariv regent inhibited hypocotyl elongation promoted by GA and IAA treannent in intact cucumber seedling and hypocotyl segments of azuki bean. Together with results using anti-AGP antibodies, the product of 092 was suggested to be an AGP. The transgenic tobacco plants showed earlier flowering than wide ty'pe plants.

著者

和田 七夕子 勝見 允行

出版者

植物化学調節学会

雑誌

植物化学調節学会研究発表記録集 (ISSN:09191887)

巻号頁・発行日

no.35, pp.85-86, 2000-11-02

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From the 7th internodes of light-grown seedlings of LKB pea (wild type) and 1kb dwarf pea, a brassinolide (BR) deficient mutant, upper 8 mm segments were excised and stored in 50% glycerol at -15C for more than 2 weeks. The extensibility (φ) and the yield threshold (y) of the cell wall were analyzed by creep measurements of these glycerinated segments (G-segments). LKB had higher φ and lower y values than lkb in response to acidity (pH4.5). However, G-segments taken from BR-treated 1kb seedlings had φ and y values similar to those of LKB. On the other hand, G-segments of LKB taken from seedlings treated with brassinazole (BZ), an inhibitor of BR biosynthesis, had higher y values than non-treated LKB. When G-segments of lkb were incubated with BR for 1 hr and then creep measurements were made in the presence of BR, their φ and y values were also similar to those of LKB. Heat treatment of G-segments diminished their ability to respond to acidity. Addition of a crude cell wall protein fraction to heat-treated LKB G-segments could restore their ability. In the case of heat-treated 1kb and LKB pretreated with BZ, their ability could be restored only in the presence of BR. These results suggest that BR is necessary for acid-induced cell wall loosening possibly as an effector of cell wall enzymes responsible for φ and y changes.

著者

板井 章浩 新田 順子

出版者

植物化学調節学会

雑誌

植物化学調節学会研究発表記録集 (ISSN:09191887)

巻号頁・発行日

no.44, 2009-10-06

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There is a large cultivar difference in ethylene synthesis during fruit ripening in Japanese pear. Fruit storage potential is closely related to maximum level of ethylene production in Japanese pear. PPACS2 was specifically expressed in cultivars showing moderate ethylene production. We have found that PpACS2 expression can be controlled by regions comprising purine-rich (GAGA repeats) sites that are located near TATA box. PpACS2 promoter of moderate ethylene producers has either (GA)_<14> or (GA)_<10>. On the other hand, that of low ethylene producers has only (GA)_5. Recently, genes have been identified that encode a class of proteins (GABP) that binds GA-rich elements in three plant species. To identify factors that interact with the promoter regions of PpACS2, southwestern screening and 5' and 3' RACE methods following RT-PCR with degenerate primers were performed. We cloned two possible GABPs (PpRTF1 and PpGABP2) and studied their expression during fruit development and ripening. To test whether both genes are able to bind to GA repeats, eletcromobility shift assays (EMSAs) were performed. PpRTF1 protein has shown to bind (GA)_<14>, but PpGABP2 has not. Furthermore, PpRTF1 protein has the ability to bind nucleotides consisting of (GA)_<10>, but no binding activity of nucleotides consisting of (GA)_5. These results suggest that PpRTF1 protein can be involved in the regulation of PpACS2 expression.

著者

花井 秀俊 斉藤 千秋 石田 小百合 米田 哲也 草野 都 田母神 繁 野間 正名

出版者

植物化学調節学会

雑誌

植物化学調節学会研究発表記録集 (ISSN:09191887)

巻号頁・発行日

no.38, 2003-10-10

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When supplemented to culture medium of mushroom Coprinus cinereus, rice husks which had been soaked in methanol beforehand dose-dependently stimulated mycelia growth up to a concentration of 80mg/ml, but nontreated husks up to 20mg/ml. These results suggested the existence of both stimulatory (hydrophilic) and inhibitory (lipophilic) compounds in rice husks. As momilactone A (MLA) had been isolated as one of germination inhibitors in rice husks, its biological activity against mycelia growth was tested. Momilactone A inhibited the mycelia growth at a concentration of 1μg/disc. Methanol extract of the husks also inhibited at a concentration of 1mg/disc (corresponding to 0.2μg MLA/disc, by quantification with LC/MS/MS). Thus, lipophilic compounds such as MLA in rice husks, readily extractable with methanol (two days extraction at room temperature or over one hour reflux), should inhibit the mycelia growth. Aqueous extract of methanol soaked husks stimulated mycelia growth. Purification of the stimulatory compounds on ion exchange- and gel permeation columns followed by RP-HPLC is not enough to yield pure active principals. Rice husks also stimulated mycelia growth of Grifola frondose, Lentinus edodes, Pleurotus eriyngii and P. ostreatus as well as C. cinereus. Furthermore, they increased both the number and fresh weight of primordia and adult fruit body of P. eriyngii and P. ostreatus when supplemented to the medium.