著者

天野 裕治

出版者

九州歯科学会

雑誌

九州齒科學會雜誌 : Kyushu-Shika-Gakkai-zasshi (ISSN:03686833)

巻号頁・発行日

vol.46, no.1, pp.319-328, 1992-02-25

被引用文献数

1

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Using the technique of in vivo microdialysis, extracellular concentrations of serotonin and 5-hydroxyindoleacetic acid (5-HIAA) were determined in the striatum, thalamus, dentate gyrus and prefrontal cortex while Sprague-Dawley rats underwent immobilization stress for twenty minutes. Microdialysis probes had semipermeable cellulose tubes (molecular weight cutoff 50, 000 ; O.D. 0.22 mm). Perfusion was started at 12 or 36 hours after implantation of the microdialysis probe under a freely-moving condition. Measurements of the extracellular concentration of serotonin and 5-HIAA were made at twenty minute intervals, which were started 120 minutes after the onset of the perfusion. Serotonin and 5-HIAA were quantified by a high-performance liquid chromatography coupled with an electrochemical detector. Extracellular serotonin levels increased following twenty minute immobilization stress in all four regions of the rat brain. The immobilization stress relatively increased the extracellular serotonin levels in the prefrontal cortex and dentate gyrus. These results suggest that twenty minute immobilization stress induces the activation of serotonin release in rat brain regions concerning the induction of anxiety.