著者

ICHISHIMA Eiji YAMAGATA Youhei CHIBA Hideki SAWAGUCHI Kazuyo TANAKA Tomoaki

出版者

社団法人日本農芸化学会

雑誌

Agricultural and Biological Chemistry (ISSN:00021369)

巻号頁・発行日

vol.53, no.7, pp.1867-1872, 1989-07-23

被引用文献数

1 12

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An aminopeptidase, Ey, is a newly discovered enzyme of hen's egg yolk. About 95% of the total activity was found in the yolk plasma fraction, with most of the remainder in the yolk granule fraction. The highly purified fraction from plasma showed about 13,000-fold higher specific activities than that of yolk plasma. Polyacrylamide gel electrophoresis revealed that the enzyme was homogeneous. The bound enzyme in the granule was extracted with 3% NaCl and was partially purified. The soluble and bound forms of aminopeptidase differ in solubility, molecular weight, heat stability, optimal pH, and Km.