- 著者
- ICHISHIMA Eiji YAMAGATA Youhei CHIBA Hideki SAWAGUCHI Kazuyo TANAKA Tomoaki
- 出版者
- 社団法人日本農芸化学会
- 雑誌
- Agricultural and Biological Chemistry (ISSN:00021369)
- 巻号頁・発行日
- vol.53, no.7, pp.1867-1872, 1989-07-23
- 被引用文献数
- 1 12
An aminopeptidase, Ey, is a newly discovered enzyme of hen's egg yolk. About 95% of the total activity was found in the yolk plasma fraction, with most of the remainder in the yolk granule fraction. The highly purified fraction from plasma showed about 13,000-fold higher specific activities than that of yolk plasma. Polyacrylamide gel electrophoresis revealed that the enzyme was homogeneous. The bound enzyme in the granule was extracted with 3% NaCl and was partially purified. The soluble and bound forms of aminopeptidase differ in solubility, molecular weight, heat stability, optimal pH, and Km.