著者

Munehiro YOSHIDA Kyoden YASUMOTO Kimikazu IWAMI Hiroyuki TASHIRO

出版者

Japan Society for Bioscience, Biotechnology, and Agrochemistry

雑誌

Agricultural and Biological Chemistry (ISSN:00021369)

巻号頁・発行日

vol.45, no.7, pp.1681-1688, 1981 (Released:2006-03-27)

参考文献数

29

被引用文献数

6

---

A major proportion of selenium in bovine milk was found in fluorornetric analysis to be associated with the casein fraction, largely in alkali-labile form, and the rest with the whey fraction mostly in free selenite form. This uneven distribution of milk selenium seems to provide an explanation for selenium deficiency in purified caseins. The activity of glutathione peroxidase, a selenoprotein, in the liver of growing male rats fed ad libitum low-selenium diets containing either vitamin-free casein or Torula yeast 0.065 ±0.012 or 0.015 ±0.004μg Se/g diet, respectively) for 3 weeks decreased to 4 to 6% of that of the control rats fed a commercial stock diet (0.185±0.092fig Se/g diet). Selenium status was evaluated by three different parameters for the rats assigned under pair-feeding regimen to those vitamin-free casein-based diets which were supplemented with graded levels of selenium as sodium selenite. The hepatic levels of the thiobarbituric acid-reactive substance, an indication of lipid peroxidation, decreased to control level with selenium supplementation per g diet of 0. 1 μg and over. The hepatic glutathione peroxidase activity reached a plateau above a 0. 1 μg/g diet of selenium supplementation, whereas the erythrocyte enzyme activity increased with increasing levels of supplementary selenium. These results support the notion that semi-purified diets containing vitamin-free casein as a prime protein source would not satisfy the selenium requirement of growing animals unless deliberately supplemented with additional selenium.

著者

Reiichiro SAKAMOTO Motoo ARAI Sawao MURAO

出版者

Japan Society for Bioscience, Biotechnology, and Agrochemistry

雑誌

Agricultural and Biological Chemistry (ISSN:00021369)

巻号頁・発行日

vol.49, no.5, pp.1283-1290, 1985 (Released:2006-03-27)

参考文献数

27

被引用文献数

12 21

---

The enzymic properties of three electrophoretically distinct β-glucosidases, β-glucosidase-1, -2 and -3, from Aspergillus aculeatus No. F-50 were investigated. β-Glucosidase-3 had a low optimum pH of 3.0, but the other two enzymes had optimum pHs of 4.0-4.5. Both β-glucosidase-1 and -2 were potently active not only on soluble cellooligosaccharides, such as cellotriose to cellohexaose, but also on insoluble cellooligosaccharide, of which the average degree of polymerization was 20. On the contrary, β-glucosidase-3 was only slightly active on the insoluble substrate. The combined use of either β-glucosidase-1 or -2 and endo-glucanase remarkably stimulated the hydrolysis of amorphous cellulose, yielding glucose only. But β-glucosidase-3 did not show such a synergistic effect, and the glucose content of the hydrolyzate was only about 60%.

著者

Kiyoshi EBIHARA Akira NAKAJIMA

出版者

Japan Society for Bioscience, Biotechnology, and Agrochemistry

雑誌

Agricultural and Biological Chemistry (ISSN:00021369)

巻号頁・発行日

vol.52, no.5, pp.1311-1312, 1988 (Released:2006-04-05)

参考文献数

7

被引用文献数

21

著者

Shinichi KINOSHITA Sadao KAGEYAMA Kazuhiko IBA Yasuhiro YAMADA Hirosuke OKADA

出版者

Japan Society for Bioscience, Biotechnology, and Agrochemistry

雑誌

Agricultural and Biological Chemistry (ISSN:00021369)

巻号頁・発行日

vol.39, no.6, pp.1219-1223, 1975 (Released:2008-11-27)

参考文献数

13

被引用文献数

11 37

---

A microorganism, strain KI 72 capable of utilizing ε-aminocaproic acid cyclic dimer as sole carbon and nitrogen sources was isolated from sludge and identified as Achromobacter guttatus. This bacteria utilized 1% of the cyclic dimer in a day and was not inhibited by the higher concentration of the dimer. The growth rate was independent of the cyclic dimer concentration in the medium, but the maximum cell concentration increased with the increase of substrate concentration. The cell yield was 0.7mg dry cell/mg ε-aminocaproic acid cyclic dimer. Bacterial growth with the cyclic dimer as substrate was significantly stimulated by the addition of yeast extract. Ferric chloride was also stimulatory. Maximal growth was obtained in cultures incubated at pH 6 and at 33°C. Synthesized nylon oligomers, ranging from ε-aminocaproic acid up to its linear hexamer, were found to be catabolized by this organism.

著者

SAKURAI Kazutoshi IKEDA Koichiro MORI Kenji

出版者

社団法人日本農芸化学会

雑誌

Agricultural and Biological Chemistry (ISSN:00021369)

巻号頁・発行日

vol.52, no.9, pp.2369-2371, 1988-09-23

被引用文献数

2 16

著者

Fumio YAGI Kenjiro TADERA

出版者

Japan Society for Bioscience, Biotechnology, and Agrochemistry

雑誌

Agricultural and Biological Chemistry (ISSN:00021369)

巻号頁・発行日

vol.51, no.6, pp.1719-1721, 1987 (Released:2006-04-05)

参考文献数

11

被引用文献数

9

著者

Ritsuo NISHIDA Chul-Sa KIM Hiroshi FUKAMI Ryozo IRIE

出版者

Japan Society for Bioscience, Biotechnology, and Agrochemistry

雑誌

Agricultural and Biological Chemistry (ISSN:00021369)

巻号頁・発行日

vol.55, no.7, pp.1787-1792, 1991 (Released:2006-04-05)

参考文献数

14

被引用文献数

19

---

Two new pyrrolizidine alkaloids, ideamines A and B, together with other analogs (lycopsamine and parsonsine) were isolated in the N-oxide forms from adult bodies of the Apocynaceae-feeding danaine butterfly, Idea leuconoe. Ideamine A was characterized as a homolog of lycopsamine, in which the viridifloric acid moiety was replaced by a 2-ethyl-2, 3-dihydroxybutanoic moiety. Likewise, ideamine B was identified as a nor-derivative of parsonsine, in which the trachelanthic acid moiety was replaced by a 2-ethyl-2, 3-dihydroxybutanoic moiety diastereomeric to the necic acid from ideamine A.

著者

ICHISHIMA Eiji YAMAGATA Youhei CHIBA Hideki SAWAGUCHI Kazuyo TANAKA Tomoaki

出版者

社団法人日本農芸化学会

雑誌

Agricultural and Biological Chemistry (ISSN:00021369)

巻号頁・発行日

vol.53, no.7, pp.1867-1872, 1989-07-23

被引用文献数

1 12

---

An aminopeptidase, Ey, is a newly discovered enzyme of hen's egg yolk. About 95% of the total activity was found in the yolk plasma fraction, with most of the remainder in the yolk granule fraction. The highly purified fraction from plasma showed about 13,000-fold higher specific activities than that of yolk plasma. Polyacrylamide gel electrophoresis revealed that the enzyme was homogeneous. The bound enzyme in the granule was extracted with 3% NaCl and was partially purified. The soluble and bound forms of aminopeptidase differ in solubility, molecular weight, heat stability, optimal pH, and Km.

著者

ISONO Yoshinobu OKAMURA Emiko FUJIMOTO Teruo HASHIMOTO Yutaka WATANABE Toshiyuki

出版者

社団法人日本農芸化学会

雑誌

Agricultural and Biological Chemistry (ISSN:00021369)

巻号頁・発行日

vol.54, no.11, pp.2949-2952, 1990-11-23

被引用文献数

2 3

---

The apparent viscosity development after onset of steady shear flow was measured at 90℃ for the various samples of mochi cake prepared by a kneading or a stamping method. The typical mochi cake samples showed clear stress overshoot phenomena. The results agreed well with those in a sensory test. This indicates the present nonlinear viscoelastometry is very effective for characterization of mochi cake.

著者

Saburo ITO Yasuyoshi OSHIMA

出版者

Japan Society for Bioscience, Biotechnology, and Agrochemistry

雑誌

Agricultural and Biological Chemistry (ISSN:00021369)

巻号頁・発行日

vol.26, no.3, pp.156-161, 1962 (Released:2011-02-07)

参考文献数

11

被引用文献数

8

---

Kaki tannin was isolated from methanol extracts of unripe kaki fruit (Japanese persimmon) and it was found that a main constituent composing kaki tannin was a leucoanthocyanin. Its acetate and methyl ether were prepared and their properties and reactions were studied. From the results of these experiments, kaki tannin was presumed to be a (+) leucodelphinidin-3-glucoside (5, 7, 3', 4', 5'-pentahydroxyflavan-3, 4-diol-3-glucoside).

著者

Masahiro TAMURA Tohru NAKATSUKA Makoto TADA Yoshihiro KAWASAKI Eiichi KIKUCHI Hideo OKAI

出版者

Japan Society for Bioscience, Biotechnology, and Agrochemistry

雑誌

Agricultural and Biological Chemistry (ISSN:00021369)

巻号頁・発行日

vol.53, no.2, pp.319-325, 1989 (Released:2006-04-05)

参考文献数

6

被引用文献数

27

---

"Delicious peptide" was reported to be as delicious as beef soup. The peptide and its fragments were synthesized to study its taste active sites. "Delicious peptide" was found to produce a umami and a sour taste. By preparing several di- or tripeptides composed of basic or acidic amino acids, we found that the taste of "delicious peptide" was produced by an interaction between the basic and the acidic fragments in the peptide.

著者

Katsumi YAMAGUCHI Hiroshi NAKANO Masahiro MURAKAMI Shoji KONOSU Osamu NAKAYAMA Midori KANDA Akihiro NAKAMURA Hiroaki IWAMOTO

出版者

Japan Society for Bioscience, Biotechnology, and Agrochemistry

雑誌

Agricultural and Biological Chemistry (ISSN:00021369)

巻号頁・発行日

vol.51, no.2, pp.493-498, 1987 (Released:2006-04-05)

参考文献数

33

被引用文献数

28

---

Botryococcus braunii strain Austin and strain Berkeley cultured under the same conditions contained 27.4g and 34.0g of lipids per 100g of dry alga, respectively. The lipids in the former strain were 25.5% hydrocarbons, 58.8% other nonpolar lipids, and 15.7% polar lipids, and those in the latter strain were 71.6%, 9.2%, and 19.2%, respectively. Distinct differences were also observed in the components of the nonpolar and polar lipids, especially in the latter, between the strains. The fatty acid composition of the nonpolar lipid fraction in Austin strain was unique, oleic acid being 81% of the total acids.

著者

Kazuhide KIMBARA Toshiyuki HASHIMOTO Masao FUKUDA Takao KOANA Masamichi TAKAGI Michio OISHI Keiji YANO

出版者

Japan Society for Bioscience, Biotechnology, and Agrochemistry

雑誌

Agricultural and Biological Chemistry (ISSN:00021369)

巻号頁・発行日

vol.52, no.11, pp.2885-2891, 1988 (Released:2006-04-05)

参考文献数

27

被引用文献数

19

---

A mixed culture composed of two Pseudomonas strains, designated as KKL101 and KKS102, was isolated from soil. This mixed culture had an enhanced ability to degrade various polychlorinated biphenyls (PCBs) which include highly chlorinated components. They did not grow individually on the mineral salts medium supplemented with a highly chlorinated PCB (PCB48, a mixture of mainly tetrachlorobipheny1) and biphenyl. When the spent medium of KKL101 was added to the washed cell preparation of KKS102, however, the latter grew on these carbon sources, producing yellow compounds which were identified as metabolic intermediates of the carbon sources, biphenyl and PCBs. These results suggest that KKL101 produces a growth factor(s) essential for KKS102 to grow on PCBs and that the growth of KKL101 is supported by the metabolic intermediates produced by KKS102. It appears that these two bacterial strains have a symbiotic relationship. From the analysis of the degradation products of various PCB congeners, it was found that strain KKS102 degrades a wide range of PCBs which have been considered to be refractory to biological degradation.

著者

Minoru ISOBE Duangchan UYAKUL Jon B. SIGURDSSON Toshio GOTO Toong J. LAM

出版者

Japan Society for Bioscience, Biotechnology, and Agrochemistry

雑誌

Agricultural and Biological Chemistry (ISSN:00021369)

巻号頁・発行日

vol.55, no.8, pp.1947-1951, 1991 (Released:2006-04-05)

参考文献数

13

---

The land snail, Dyakia striata, bioluminesces to exhibit green light. Its luminescence spectrum was recently reported by using juvenile snails to show the maximum at ca. 515 nm. In the current studies, a green fluorescent substance was extracted from the separated anterior head-foot of the adult snails, which contained the photogenic organs. The fluorescent substance, which showed the maximum ca. 515nm in its spectrum, was purified by chromatography. The partially purified green fluorescent substance was soluble only in organic solvents and not in water. The chromophore of the green fluorescent substance was compared with isoalloxazine and riboflavin acetate in different organic solvents under reductive and re-oxidative conditions.

著者

ENDO Hiroshi NAKAJIMA Kei CHINO Ryuji SHIROTA Minoru

出版者

公益社団法人 日本農芸化学会

雑誌

Agricultural and Biological Chemistry (ISSN:00021369)

巻号頁・発行日

vol.38, no.1, pp.9-18, 1974

被引用文献数

28

---

A <i>Chlorella</i> strain with high growth rates under heterotrophic growth condition was isolated from a pond and identified as <i>Chlorella regularis.</i> The optimal temperature for growth was 36&deg;C and the optimal pH 6.0_??_7.0. Glucose, galactose, acetic acid, ethanol, acetaldehyde and pyruvic acid may serve as the carbon source supporting the growth under both dark and light conditions.<br> The specific growth rate &mu;max was; 0.3 hr^-1 under autotrophic condition, 0.28 hr^-1 under heterotrophic condition, and 0.45 hr^-1 under mixotrophic condition. In the dark heterotrophic culture, the yield of the cells to glucose was 0.5_??_0.6, that to acetic acid 0.48, and that to ethanol 0.66. When dark heterotrophic cells were cultured under mixotrophic condition, the yield to glucose increased to 0.82, that to acetic acid to 0.85 and to ethanol to 0.91.<br> The protein and chlorophyll contents of the cells in autotrophic culture were approximately, 60% and 4Y., respectively. These contents of the cells in dark heterotrophic culture changed in accordance with the growth phase. The protein of the cells reached to, approximately, 60% and the chlorophyll to 2% in stationary phase of growth, although the cells were maintained even in the dark.

著者

Michiko WATANABE Norikazu FUJII Soichi ARAI

出版者

Japan Society for Bioscience, Biotechnology, and Agrochemistry

雑誌

Agricultural and Biological Chemistry (ISSN:00021369)

巻号頁・発行日

vol.46, no.6, pp.1587-1592, 1982 (Released:2006-03-27)

参考文献数

11

被引用文献数

9

---

A papain-catalyzed reaction involving covalent incorporation of L-leucine n-alkyl ester is available for producing an enzymatically modified protein (EMP) with surfactancy [Agric. Biol. Chem., 45, 1621 (1981)]. In the present work we used gelatin as a starting material and incorporated L-leucine n-hexyl ester to produce a whippable EMP and L-leucine n-dodecyl ester to produce an emulsifiable EMP. A foam system formed with the whippable EMP was much stabler than that formed with sodium dodecylsulfate. The emulsifiable EMP also gave a much stabler oil-in-water emulsion than Tween-80 did. The stability of the emulsion formed with EMP was not affected by the presence of NaCl at a very high concentration. The observed foam and emulsion stabilities were well explained by the data for decreased mobility of the involved water protons. These results may indicate that EMP molecules, when arranged at the air/water or oil/water interface, can bind a part of the water to form thick barriers which prevent the air or oil particles from coalescing.

著者

Misao TASHIRO Zensuke MAKI

出版者

Japan Society for Bioscience, Biotechnology, and Agrochemistry

雑誌

Agricultural and Biological Chemistry (ISSN:00021369)

巻号頁・発行日

vol.42, no.6, pp.1119-1124, 1978 (Released:2008-11-27)

参考文献数

15

被引用文献数

13

---

A trypsin inhibitor was extracted from rice bran with 1% sodium chloride and partially purified by 40_??_80% ammonium sulfate fractionation. The crude inhibitor obtained showed the inhibitory activity on trypsin [EC 3. 4. 21. 4] but not on a-chymotrypsin [EC 3. 4. 21. 1] or pepsin [EC 3. 4. 23. 1]. This inhibitor was stable at acidic and neutral pHs but was gradually inactivated by the long-time incubation with pepsin. The molecular weight of the inhibitor was estimated to be 13, 500 by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. Furthermore, gel filtration on Sephadex G-75 suggested the possible self-association of the inhibitor. Isoelectric focusing demonstrated that the inhibitor is a basic protein having a pI value of 8.2. The inhibitor was adsorbed on to CM-Sephadex C-25 at pH 5.7, indicating that it can be further purified by this chromatography.

著者

Teiichiro ITO Akira TAKATSUKI Kenji KAWAMURA Katsuki SATO Gakuzo TAMURA

出版者

Japan Society for Bioscience, Biotechnology, and Agrochemistry

雑誌

Agricultural and Biological Chemistry (ISSN:00021369)

巻号頁・発行日

vol.44, no.3, pp.695-698, 1980 (Released:2008-11-27)

参考文献数

11

被引用文献数

44

著者

Kazuo YOSHIOKA Naoki HASHIMOTO

出版者

Japan Society for Bioscience, Biotechnology, and Agrochemistry

雑誌

Agricultural and Biological Chemistry (ISSN:00021369)

巻号頁・発行日

vol.48, no.2, pp.333-340, 1984 (Released:2006-03-27)

参考文献数

12

被引用文献数

15

---

Formation of acetate esters by brewers' yeast during sugar fermentation was investigated in relation to alcohol acetyltransferase activity influenced by the fatty acid composition of the yeast cell membrane. Glucose gave more acetate esters with a higher activity of alcohol acetyltransferase than the other carbohydrates. When maltose was fermented, the activity of alcohol acetyltransferase bound to the cell membrane was suppressed by unsaturated fatty acids accumulated in the cell membrane and the formation of acetate esters was greatly reduced without insufficient fermentation. On the other hand, when fructose was fermented, the ester formation was reduced with a decrease in the enzyme activity and the formation of higher alcohols through insufficient fermentation.

著者

MORITA Kazuyoshi HARA Masako KADA Tsuneo

出版者

公益社団法人 日本農芸化学会

雑誌

Agricultural and Biological Chemistry (ISSN:00021369)

巻号頁・発行日

vol.42, no.6, pp.1235-1238, 1978

被引用文献数

70

---

Juice prepared from cabbage, broccoli, green pepper, egg plant, apple, burdock, shallot, ginger, pineapple and mint leaf were found to possess strong capacities of inactivating the mutagenicity of tryptophane pyrolysis products. In addition, radish, sweet potato, grape, Japanese ginger, cauliflower, beefsteak plant, enokidake mushroom and simeji mushroom were moderatly effective. The other forty one samples were inactive. Among the above eleven samples that inactivated the tryptophan pyrolysate efficiently, egg plant, burdock and broccoli showed the widest spectra of inactivating other mutagenic amino acids pyrolysates. The desmutagenic factor of cabbage is sensitive to heating (100&deg;C) and pronase treatment, sug-gesting a nrotein character.