Our aim is to produce ethanol efficiently by enzymatic saccharification coupled with ethanol fermentation using bamboo plant as a biomass. For this purpose, we examined pretreatment conditions of biomass for saccharification. At first bamboo plants were made into chips and then powders. When a distilled water with bamboo powder was 60-min autoclaved at 121 ℃, 0.75 M NaOH-alkalization treatment was performed at 80 ℃ , and saccharification was carried out by 0.33 g/ ℓ Acremonium cellulose at 45 ℃ , glucose was released at 5.7 g/ℓ after 96-h saccharification, which corresponded to 22.8 % on the basis of the the bamboo plant. When fermentation was carried out at 30 ℃ following 96-h saccharification of the pretreated bamboo plant using Acremonium cellulase, the inhibitory effect of glucose was considered to be reduced and ethanol was produced at 4.2 g/ℓ after 72-h fermentation, which corresponded to 50.8 % yield on the basis of the total amount of bamboo.