著者

梅田 正明 大坪 久子 大坪 栄一

出版者

Genetics Society of Japan

雑誌

遺伝学雑誌 (ISSN:0021504X)

巻号頁・発行日

vol.66, no.5, pp.569-586, 1991

被引用文献数

18 70

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The <i>waxy</i> (<i>wx</i>) gene of <i>Oryza glaberrima</i> was cloned, and its nucleotide sequence was determined. A waxy mutant of <i>O. glaberrima</i> showing a glutinous phenotype was found to contain a substitution mutation generating a termination codon in the coding region of the <i>wx</i> gene. The <i>Wx</i> sequence of <i>O. glaberrima</i> was different from that of <i>Oryza sativa</i> by substitutions and insertions/deletions, among which only a few substitutions occurred in several exons not to severely alter the amino acid sequence of the <i>Wx</i> protein. The most striking difference observed in introns was a 139-bp deletion (or insertion) in intron 10 of <i>O. glaberrima</i> (or <i>O. sativa</i>). In <i>O. sativa</i>, 125bp of the 139-bp sequence was flanked by direct repeats of a 14-bp sequence. A sequence homologous to the 125-bp sequence was found in the region preceding exon 2; this sequence was also flanked by direct repeats of another 14-bp sequence. This result and the observation that the 125-bp sequence was interspersed in rice genomes indicate that they are SINEs (short interspersed elements) in the plant system. We also identified a DNA sequence with long terminal inverted repeats in intron 13 of both <i>O. glaberrima</i> and <i>O. sativa</i>. This sequence was present in multiple copies in rice genomes, suggesting that it is a transposable element. These results obtained suggest that mobile DNA elements have diversified the rice <i>Waxy</i> gene by inserting into introns, each of which may originally have a length of about 100 bp.<br>