著者

佐野 順一 田中 正武

出版者

Genetics Society of Japan

雑誌

遺伝学雑誌 (ISSN:0021504X)

巻号頁・発行日

vol.55, no.1, pp.9-17, 1980

被引用文献数

3

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In order to estimate chromosomal homology between <i>Aegilops</i> <i>speltoides</i> (genomic constitution SS) and the tetraploid wheats (AABB or AAGG), effects of the B-chromosomes and genotypes of <i>Ae. speltoides</i> on chromosome pairing at MI of meiosis in F₁ hybrids of <i>Ae. speltoides</i> × tetraploid wheats were investigated.<br>In hybrids with B's, chromosome pairing was strikingly affected by the B's and very small amount of pairing was observed. In hybrids without B's, however, extensive pairing was observed, although the amount of pairing varied due to the <i>speltoides</i> genotypes involved. In these hybrids with or without B's, although some minor yet distinctive differences in pairing attributable to the kind of genomic constitution of the wheat parent were observed in the multivalent frequency and in the distribution (or terminalization) of chiasmata, both hybrids with different genomic constitution (SAB or SAG) had essentially the same amount of pairing. Since the B-chromosomes used did not cause asynapsis (or desynapsis) of homologous chromosomes in <i>Ae. speltoides</i>, the pairing reduced by the presence of B's in the hybrids does not seem to be homologous and is probably homoeologous. Therefore, it can be concluded that there is little chromosomal homology between <i>Ae. speltoides</i> and both tetraploid wheats of genomic constitution AABB and AAGG.

著者

庄武 孝義 野沢 謙 田名部 雄一

出版者

Genetics Society of Japan

雑誌

遺伝学雑誌 (ISSN:0021504X)

巻号頁・発行日

vol.52, no.3, pp.223-237, 1977

被引用文献数

35

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In central Ethiopia the hamadryas baboon, <i>Papio hamadryas</i> and the anubis baboons, <i>Papio anubis</i>, have been hybridizing naturally. Studies were made by using electrophoretical blood protein variations as markers in order to clarify the genetic interrelationships between them. A total of 414 individuals belonging to 7 populations were examined for 35 blood protein loci. Eleven of 35 loci showed polymorphism. The Tf locus was found to have the strongest power for discriminating between the anubis and the hamadryas. The results of our genetic survey revealed that most of the populations from which we collected blood samples were more or less hybridized. The Nei's (1975) genetic distance between the two species was estimated to be at most as 0.0674. As this value was too small to consider these species as real biological species, we think that the natural hybrid zone is fairly wide and still expanding now.

著者

TSUNEWAKI K. KASAHARA F. FUJITA T.

出版者

Genetics Society of Japan

雑誌

遺伝学雑誌 (ISSN:0021504X)

巻号頁・発行日

vol.46, no.2, pp.103-107, 1971

被引用文献数

2 4 6

---

One hundred and eighty-nine Chinese cultivars of common wheat were crossed to three testers for necrosis and chlorosis genes, and their genotypes were determined. <br>Three genotypes, <i>Ne</i>₁<i>ne</i>₂, <i>ne</i>₁<i>Ne</i>₂ and <i>ne</i>₁<i>ne</i>₂ for necrosis were found in 39.3, 7.1 and 53.6% of the cultivars, respectively. As to chlorosis genes, the great majority of cultivars (93.4%) were of the genotype, <i>ch</i>₁<i>Ch</i>₂, the remainder (6.6%) were <i>ch</i>₁<i>ch</i>₂. It was evident that common wheat retained its characteristic population structure regarding necrosis and chlorosis during its eastward dispersion from Central Asia to Japan through China. No particular geographical inclination in the frequencies of various necrosis and chlorosis genotypes, nor their special association with the two characters, growth habit and awnedness, was detected.

著者

大西 近江 西本 登志

出版者

Genetics Society of Japan

巻号頁・発行日

vol.63, no.1, pp.51-66, 1988 (Released:2011-12-08)

著者

大西 近江 西本 登志

出版者

Genetics Society of Japan

雑誌

The Japanese journal of genetics (ISSN:0021504X)

巻号頁・発行日

vol.63, no.1, pp.51-66, 1988-02

著者

鈴木 仁 HOSODA Tetsuji SAKURAI Susumu TSUCHIYA Kimiyuki MUNECHIKA Isao KORABLEV Vladimir P.

出版者

Genetics Society of Japan

雑誌

The Japanese journal of genetics (ISSN:0021504X)

巻号頁・発行日

vol.69, no.4, pp.397-406, 1994-08-25

被引用文献数

7 9

---

We analyzed the restriction fragment length polymorphisms in the spacer regions of ribosomal DNA (rDNA), using twelve restriction enzymes, to examine whether the Iriomote cat is related to the leopard cat (<i>Felis bengalensis</i>). A restriction map for each taxon was constructed and the major taxon-specific types of repeating unit (repetypes) were characterized on the basis of the arrangements of restriction sites. The Iriomote cat and the leopard cat share a common repetype but this repetype is different from that of the domestic cat (<i>F. catus</i>) with an estimated sequence divergence of 1.5% and from that of the ocelot (<i>F. paradalis</i>) with an estimated sequence divergence of 2.5%. These results indicate that, phylogenetically, the Iriomote cat is closely related to the leopard cat and that the ancestral population moved from the continent to Iriomote Island quite recently. The rDNA arrays of the leopard cat exhibit considerable intragenomic size-variation, which is thought to have emerged as a result of differences in numbers of repeated DNA segments, whereas the extent of such size-variation is much lower in the rDNA of the Iriomote cat. It appears that, even though migration of the Iriomote cat occurred relatively recently, the population has diverged to some extent from its continental counterpart, perhaps via fixation of preexistent intraspecific variations rather than by generation of new variations.<br>

著者

市川 定夫 今井 敏彦 中野 篤

出版者

Genetics Society of Japan

雑誌

The Japanese journal of genetics (ISSN:0021504X)

巻号頁・発行日

vol.66, no.4, pp.513-525, 1991-08

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Induced somatic pink mutation frequencies in the stamen hairs of Tradescantia KU 20 clone, a blue/pink heterozygote highly mutable spontaneously at lower temperature, were studied after treating with relatively small doses of Co-60 gamma rays (39 to 551 mGy or 3.9 to 55.1 rad), and were compared with those of two stable clones (non-mutable spontaneously), BNL 02 and KU 9, which are also blue/pink heterozygotes. It was found that the gamma-ray-induced mutation frequency in KU 20 clone was comparable (18.8 pink mutant events per 10(4) hair-cell divisions per Gy) to those in BNL 02 (12.2 and 21.2) and KU 9 (17.4) clones, when the spontaneous mutation frequencies of KU 20 clone were relatively low (at most about 5.7 and 2.3 times of BNL 02 and KU 9 clones, respectively). However, when the spontaneous mutation frequecies of KU 20 clone were much higher (up to about 65 and 27 times of BNL 02 and KU 9 clones, respectively), induced mutation frequency was significantly higher in KU 20 clone (58.8 pink mutant events per 10(4) hair-cell divisions per Gy) than in BNL 02 and KU 9 clones. The extent of increase in the gamma-ray-induced mutation frequency in the latter case was nevertheless very much less than the increase in the spontaneous mutation frequency, suggesting different mechanisms of initiation and repair of radiation-induced and spontaneous mutations.

著者

小原 良孝

出版者

Genetics Society of Japan

巻号頁・発行日

vol.60, no.2, pp.157-160, 1985 (Released:2014-03-27)

著者

小原 良孝

出版者

Genetics Society of Japan

雑誌

The Japanese journal of genetics (ISSN:0021504X)

巻号頁・発行日

vol.60, no.2, pp.157-160, 1985-04

著者

市川 定夫 山口 明彦 奥村 幹子

出版者

Genetics Society of Japan

雑誌

The Japanese journal of genetics (ISSN:0021504X)

巻号頁・発行日

vol.68, no.4, pp.277-292, 1993-08-25

参考文献数

25

被引用文献数

11 23

---

Young inflorescences of <i>Tradescantia</i> clones KU 27 and BNL 4430, the both of which are blue/pink heterozygotes and have been demonstrated to be highly sensitive to alkylating agents, were exposed either to aqueous solutions of methyl methanesulfonate (MMS) for 16hr alone (at 0.005 to 0.02% for KU 27 and at 0.005% for BNL 4430) or to acute 150 kVp X rays alone (161 to 531 mGy for KU 27 and 501 to 976 mGy for BNL 4430), or in combinations (134 to 448 mGy for KU 27 and 458 to 865mGy for BNL 4430 after the 0.005% MMS treatment). The induced somatic pink mutation frequencies per hair-cell division were studied and compared, and clone BNL 4430 was found to be nearly two times more sensitive to MMS than clone KU 27, while the X-ray-induced mutation frequencies in the latter was about 1.5 times higher than those in the former. The lower sensitivity to MMS of clone KU 27 (as compared with BNL 4430) was nevertheless about 5.6 times higher as compared with the responses of clone BNL 02 to MMS reported earlier, proving the high sensitivities of the two clones used in the present study. Clear synergistic effects of MMS and X rays were observed in the both clones, indicating that the mechanisms of inducing mutations are common at least in part between MMS and X rays.<br>

著者

堀 雅明

出版者

Genetics Society of Japan

雑誌

遺伝学雑誌 (ISSN:0021504X)

巻号頁・発行日

vol.55, no.1, pp.p41-54, 1980-02

著者

執行 正義 田代 洋丞 宮崎 貞巳

出版者

Genetics Society of Japan

雑誌

The Japanese journal of genetics (ISSN:0021504X)

巻号頁・発行日

vol.69, no.4, pp.417-424, 1994-08-25

被引用文献数

14 21

---

The chromosomal locations of glutamate oxaloacetate transaminase gene loci in Japanese bunching onion (<i>Allium fistulosum</i> L., 2<i>n</i>=2X= 16, FF) and shallot (<i>A. cepa</i> L. Aggregatum group, 2<i>n</i>=2X = l6, AA) were investigated using alien monosomic addition lines (2<i>n</i>=2X+1=17, FF+nA, AA+nF) and hypoallotriploid lines (2<i>n</i>=3X-1=23, AFF-nA, AAF-nF) between these two species. The gene locus <i>Got-2</i> was located on the homoeologous sub-telocentric chromosomes, 6F in <i>A. fistulosum</i> and 6A in <i>A. cepa</i> Aggregatum group. The gene locus <i>Got-1</i> was on the other chromosomes. The present results indicate that chromosomal locations of these gene loci, at least <i>Got-2</i>, were conservative both in <i>A. fistulosum</i> and <i>A. cepa</i> Aggregatum group during speciation and subsequent evolution.<br>

著者

木村 澄 OKUMURA Takashi NINAKI Osamu KIDWELL Margaret G. SUZUKI Koichi

出版者

Genetics Society of Japan

雑誌

The Japanese journal of genetics (ISSN:0021504X)

巻号頁・発行日

vol.68, no.1, pp.63-71, 1993-02-25

参考文献数

26

被引用文献数

8 6

---

As a first step in surveying transposable elements in silkworms and honeybees, hybridization analyses were carried out using 16 known families of <i>Drosophila</i> transposable elements as probes, <i>jockey</i> and <i>G</i> were the only transposable elements that hybridized with genomic DNA of either honeybees or silkworms under the conditions of this study, <i>jockey</i> hybridized with genomic DNA of both European honeybees (<i>Apis mellifera</i>) and silkworms (<i>Bombyx mori</i> and <i>Antheraea yamamai</i>) and showed significant bands in Southern blots. Banding patterns were highly polymorphic, <i>jockey</i> did not, however, hybridize with any strains of the Asian honeybee (<i>A. cerana</i>). <i>G</i> elements showed a faint signal with the Asian honeybee, but not with any other insects tested. The results suggest that, even though it has some limitations, this approach can be used in practice as a first preliminary step in surveys for the presence of transposable elements in organisms which do not have good genetic information.<br>

著者

鄭 明基 姜 順淑

出版者

Genetics Society of Japan

雑誌

The Japanese journal of genetics (ISSN:0021504X)

巻号頁・発行日

vol.70, no.3, pp.387-398, 1995-06-25

被引用文献数

3

著者

大西 近江 長久保 有之

出版者

Genetics Society of Japan

雑誌

The Japanese journal of genetics (ISSN:0021504X)

巻号頁・発行日

vol.57, no.6, pp.641-650, 1982-12 (Released:2011-12-08)

著者

大西 近江

出版者

Genetics Society of Japan

巻号頁・発行日

vol.68, no.4, pp.303-316, 1993 (Released:2011-12-08)

著者

梅田 正明 大坪 久子 大坪 栄一

出版者

Genetics Society of Japan

雑誌

遺伝学雑誌 (ISSN:0021504X)

巻号頁・発行日

vol.66, no.5, pp.569-586, 1991

被引用文献数

18 70

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The <i>waxy</i> (<i>wx</i>) gene of <i>Oryza glaberrima</i> was cloned, and its nucleotide sequence was determined. A waxy mutant of <i>O. glaberrima</i> showing a glutinous phenotype was found to contain a substitution mutation generating a termination codon in the coding region of the <i>wx</i> gene. The <i>Wx</i> sequence of <i>O. glaberrima</i> was different from that of <i>Oryza sativa</i> by substitutions and insertions/deletions, among which only a few substitutions occurred in several exons not to severely alter the amino acid sequence of the <i>Wx</i> protein. The most striking difference observed in introns was a 139-bp deletion (or insertion) in intron 10 of <i>O. glaberrima</i> (or <i>O. sativa</i>). In <i>O. sativa</i>, 125bp of the 139-bp sequence was flanked by direct repeats of a 14-bp sequence. A sequence homologous to the 125-bp sequence was found in the region preceding exon 2; this sequence was also flanked by direct repeats of another 14-bp sequence. This result and the observation that the 125-bp sequence was interspersed in rice genomes indicate that they are SINEs (short interspersed elements) in the plant system. We also identified a DNA sequence with long terminal inverted repeats in intron 13 of both <i>O. glaberrima</i> and <i>O. sativa</i>. This sequence was present in multiple copies in rice genomes, suggesting that it is a transposable element. These results obtained suggest that mobile DNA elements have diversified the rice <i>Waxy</i> gene by inserting into introns, each of which may originally have a length of about 100 bp.<br>

著者

奥島 実 SUGINO Dan KOUNO Yoshio NAKANO Shigeru MIYAHARA Junichi TODA Hisashi KUBO Shigemasa MATSUSHIRO Aizo

出版者

Genetics Society of Japan

雑誌

遺伝学雑誌 (ISSN:0021504X)

巻号頁・発行日

vol.66, no.2, pp.173-187, 1991

被引用文献数

8 18

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A bacterial strain, which assimilated dextran and water-insoluble glucan produced by <i>Streptococcus mutans</i>, was isolated from soil. The bacterium produced and secreted potent dextranase activity, which was identified as <i>Arthrobacter sp</i>. and named CB-8. The dextranase was purified and some enzymatic properties were characterized. The enzyme efficiently decomposed the water-insoluble glucan as well as dextran. A gene library from the bacteria was constructed with <i>Escherichia coli</i>, using plasmid pUC19, and clones producing dextranase activity were selected. Based on the result of nucleotide sequencing analysis, it was deduced that the dextranase was synthesized in CB-8 cells as a polypeptide precursor consisting of 640 amino acid residues, including 49 N-terminal amino acid residues which could be regarded as a signal peptide. In the <i>E. coli</i> transformant, the dextranase activity was detected mostly in the periplasmic space. The gene for the dextranase was introduced into <i>Streptococcus sanguis</i>, using an <i>E. coli-S</i>. <i>sanguis</i> shuttle vector that contained the promoter sequence of a gene for glucosyltransferase derived from a strain of <i>S. mutans</i>. The active dextranase was also expressed and accumulated in <i>S. sanguis</i> cells.<br>