著者

フォルクマー グレーフ 古屋 悦子 西風 脩

出版者

一般社団法人 日本臨床化学会

雑誌

臨床化学 (ISSN:03705633)

巻号頁・発行日

vol.5, no.2, pp.182-188, 1977

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The rate of hydrolysis of steroid glucuronides (poregnanediol-, and 17-hydroxycorticosteroid- glucuronides) is increased by an addition of sodium sulfate to the incubation medium, when usinng &beta;-glucuronidase preparations from bovine liver.<BR>But, sodium sulfate inhibits the enzyme hydrolysis of pregnanediol-, and estriol-glucuronides with preparations from Helix pomatia and from E. coli.<BR>The rate of hdrolysis of nonsteroid glucuronides (P-nitrophenyl-and phenolphthaleinglucuronides) is increased by sodium sulfate when using Preparations from bovine liver and Helix pomatia, it is inhibited with preparations from E. coli.<BR>The preparation from Helix pomatia consists of four glucuronidase fractions, none of which hydrolyzes steroid glucuronides specifically.