著者

田波 洋 山田 喜紹 田崎 忠勝

出版者

日本ウイルス学会

雑誌

ウイルス (ISSN:00426857)

巻号頁・発行日

vol.19, no.2, pp.70-76, 1969

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The rate of adsorption of a psittacosis agent (Goat Pneumonitis agent, strain Nagano) by monolayers of Earle's L cell have been investigated. It has been shown experimentally that the rate at which psittacosis agent elementary bodies (0.35&mu; diamater) arrive at a surface of the cell monolayers can be accurately predicted from the theory of Brownian motion. Proportion of particles adsorbed by the monolayers during an adsorption time of t seconds, Y_t, is given as Y_t=1.51&times;10^-3&radic;t.<br>There are good agreement between theoretical adsorption rate calculated from the above formula and experimentally obtained values within the experimental error. Therefore, we can estimate the number of total infective particles in the inoculum from the number of &ldquo;infected particles&rdquo; as shown by counting &ldquo;inclusion froming cells&rdquo; microscopically.<br>Adsorption of the psittacosis agent by L cell monolayers requires adequate concentration of cations. Optimum salt concentration for adsorption is approximately 10^-2 M. Divalent cations (Ca²⁺, Mg²⁺ Mn²⁺) accelerate adsorption more effectively than univalent cations such as Na⁺, K⁺ NH⁺₄ etc. Optimum pH of the medium for psittacosis adsorption has been found to be 6.1; between 5.5 and 6.5 there is a slight decline in the efficiency of adsorption. At either sides of these pH values adsorption rate declines markedly.