著者

内川 公人 熊田 信夫 山田 喜紹

出版者

日本衛生動物学会

雑誌

衞生動物 (ISSN:04247086)

巻号頁・発行日

vol.36, no.2, 1985-06-15

著者

山田 喜紹

出版者

日本ウイルス学会

雑誌

ウイルス (ISSN:00426857)

巻号頁・発行日

vol.23, no.3, pp.205-220, 1973

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Morphological change of a goat pneumonitis (GP) strain of <i>Chlamydia psittaci</i> in L cell monolayers induced by penicillin were investigated by electron microscopy. When the drug was added simultaneously with GP to the cell culture, its minimal inhibitory dose was estimated to be 1&mu;g (1.6 units) per ml of medium. Penicillin interefered with the growth cycle of the psittacosis organism at a concentration higher than 1&mu;g/ml by producing enlarged initial bodies 3 to 5&mu; in diameter. The abnormally enlarged initial bodies did multiply as such to some extent, but did not develop in to infective elementary bodies in the presence of an inhibitory dose of penicillin.<br>In the presence of a small dose of penicillin, ranging from 0.05 to 0.1&mu;g/ml, an excessive cytoplasmic membrane was formed within the enlarged initial bodies 15 hours or more after the onset of infection. At that time were produced a number of irregular-shaped &ldquo;miniature initial bodies&rdquo; and small empty vesicles. The miniature initial bodies had been liberated from the initial bodies by a buddinglike process.<br>Free miniature initial bodies did not develop further. In the presence of a smaller dose of penicillin, ranging from 0.025 to 0.5&mu;g/ml, clusters of electron-dense granules were formed within the maturing intermediate forms of the psittacosis organism. They were never found in the control intermediate forms grown in penicillin-free medium. The biochemical nature of those granules is not known at the moment.

著者

斉木 実 芝本 利重 百瀬 邦夫 北原 修 遠藤 優子 花村 潔 山田 喜紹 内川 公人 小島 荘明

出版者

信州医学会

雑誌

信州医学雑誌 (ISSN:00373826)

巻号頁・発行日

vol.30, no.2, pp.p245-252, 1982-04

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信州医学雑誌 30(2): 245-252(1982)

著者

小穴 こず枝 清水 環 吉田 雅子 塚田 美智代 山本 貴子 寺澤 文子 亀子 文子 山田 喜紹 沖村 幸枝 赤羽 貴行 川上 由行

出版者

信州大学

雑誌

紀要 (ISSN:03851982)

巻号頁・発行日

vol.21, pp.27-34, 1995

著者

田波 洋 山田 喜紹 田崎 忠勝 田村 茂博 村上 昇

出版者

長野県医学会

雑誌

信州医学雑誌 (ISSN:00373826)

巻号頁・発行日

vol.16, no.1, pp.68-74, 1967-02

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信州医学雑誌 16(1): 68-74(1967)

著者

田波 洋 山田 喜紹 鈴木 聖 田崎 忠勝

出版者

日本ウイルス学会

雑誌

ウイルス (ISSN:00426857)

巻号頁・発行日

vol.19, no.4, pp.121-127, 1969

被引用文献数

1

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Inhibitory effect of formycin and formycin B on the intracellular multiplication of goat pneumonitis agent (GP), a member of <i>Chlamydia psittaci</i>, was investigated. Drug was added to the nutrient fluid at two hours after inoculation to monolayers of either Earle's L or Yasumura's VERO cell lines. Infective units produced in cells cultivated in media which containd the antibiotic at varying dosage were assayed after an incubation period for 48 hours at 37&deg;C. The &ldquo;inclusion forming units&rdquo; were assayed by the &ldquo;infected cell count method&rdquo;.<br>The inhibitory action of both formycin and formycin B on GP-agent was far more effective in VERO cells than in L cells. The growth of the psittacosis agent in VERO cells was completely inhibited by formycin at a level of 1.4mcg per ml, whereas the drug did not inhibit the multiplication of the agent at the same concentration when L cells were chosen. The result may indicate that the psittacosis agent itself is resistant to these drugs, and that the inhibitory action is a result of some inhibitory effects of the host cell metabolism.<br>The inhibitory effect of formycin and formycin B on the psittacosis agent can be reversed to some extent by an addition of excessive purine or purine nucleoside to the nutrient media. Inosine, Na-inosinate, adenine or adenosine reversed the inhibitory effect of the two antibiotics. However, guanine and guanosine did not reverse the inhibitory effect. This may suggest that these drugs act as purine analogues, inhibiting certain essential metabolic pathway where adenosine may act an important role.

著者

田波 洋 山田 喜紹

出版者

日本ウイルス学会

雑誌

ウイルス (ISSN:00426857)

巻号頁・発行日

vol.14, no.3, pp.103-109, 1964

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Life cycle of goat pneumonitis agent, strain Nagano, in L cell culture was described. The developmental cycle occurs in four stages, as generally observed with PLT group of agents: (i) penetration of invaded elementary bodies into the cytoplasm. (ii) transformation of the elementary bodies to the initial bodies occurs by the 8th hour after inoculation. (iii) Initial bodies begin to multiply between the 11th and 12th hours as observed by appearance of the &ldquo;diplococci-like&rdquo; forms. Thereafter they multiply at an exponential rate, and (iv) highly infective mature particles are formed after the 21st hour. The life cycle is complete in 48 hours.<br>The results are well accounted for as that the invaded elementary bodies transform directly to the initial bodies, the vegetative forms, from which highly infective elementary bodies are developed. Which rules out the possibility that the multiplication of the agent might occur at a (hypothetical) preliminary virus-like phase, before the formation of the initial bodies.

著者

内川 公人 山田 喜紹 佐藤 潔 熊田 信夫

出版者

日本衛生動物学会

雑誌

衛生動物 (ISSN:04247086)

巻号頁・発行日

vol.35, no.3, pp.233-243, 1984

被引用文献数

3 1

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Field surveys were carried out monthly during the period from August 1981 to September 1982 to add to the basic knowledge of the vectors of tsutsugamushi disease in Nagano Prefecture, where outbreaks of the disease were reported in the spring of 1981 after a lapse of 14 years. Field mice and voles were trapped alive at stations selected mostly in grasslands in Miasa Village and Iida City. Preliminary or additional surveys were also done at irregular intervals in these areas and in Yamanouchi Town and Matsumoto City. From four kinds of field rodents examined, a total of 51,548 chiggers comprising three genera and 12 species was collected and identified. Among them, Leptotrombidium pallidum, L. intermedium, L. fuji, and Gahrliepia saduski were the predominant species in this order. L. scutellare, one of the known vectors in Japan, was not collected during the study, suggesting that L. pallidum would have played the role of vector in the recent reoccurrence of tsutsugamushi disease in Nagano Prefecture. Remarkable difference was observed among the average numbers of chiggers taken from different kinds of hosts. Conspicuous variations in host perference of some chigger species were also observed depending on the localities of collection. Habitat specificity was distinctly demonstrated by the fact that the faunal compositions of chigger species were closely related with the topographical features of the lands surveyed. Seasonal fluctuation of chiggers also differed depending on the localities of the survey. For example, average number of L. pallidum on the rodents showed a peak of occurrence in the late fall at the stations in Iida, while the same species of chigger had two peaks, one in spring and the other in autumn, at the stations in Miasa. The importance of studying the favorable habitats for the vector was discussed from the epidemiological viewpoints.

著者

田波 洋 山田 喜紹 田崎 忠勝

出版者

日本ウイルス学会

雑誌

ウイルス (ISSN:00426857)

巻号頁・発行日

vol.19, no.2, pp.70-76, 1969

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The rate of adsorption of a psittacosis agent (Goat Pneumonitis agent, strain Nagano) by monolayers of Earle's L cell have been investigated. It has been shown experimentally that the rate at which psittacosis agent elementary bodies (0.35&mu; diamater) arrive at a surface of the cell monolayers can be accurately predicted from the theory of Brownian motion. Proportion of particles adsorbed by the monolayers during an adsorption time of t seconds, Y_t, is given as Y_t=1.51&times;10^-3&radic;t.<br>There are good agreement between theoretical adsorption rate calculated from the above formula and experimentally obtained values within the experimental error. Therefore, we can estimate the number of total infective particles in the inoculum from the number of &ldquo;infected particles&rdquo; as shown by counting &ldquo;inclusion froming cells&rdquo; microscopically.<br>Adsorption of the psittacosis agent by L cell monolayers requires adequate concentration of cations. Optimum salt concentration for adsorption is approximately 10^-2 M. Divalent cations (Ca²⁺, Mg²⁺ Mn²⁺) accelerate adsorption more effectively than univalent cations such as Na⁺, K⁺ NH⁺₄ etc. Optimum pH of the medium for psittacosis adsorption has been found to be 6.1; between 5.5 and 6.5 there is a slight decline in the efficiency of adsorption. At either sides of these pH values adsorption rate declines markedly.

著者

田波 洋 山田 喜紹

出版者

日本ウイルス学会

雑誌

ウイルス (ISSN:00426857)

巻号頁・発行日

vol.24, no.2, pp.164-169, 1974

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In an electron microscopic study it was demonstrated that type C particles (L cell virions, LCV) were produced at a relatively high frequency in Earle's L cells (929 subline) when these cells had been incubated in the presence of 5-bromodeoxyuridine (BuDR) at a concentration of 10^-4M. LCV appeared within the intracytoplasmic vesicles of L cells at times later than 20 hours after the initiation of BuDR treatment. LCV particles were produced from the limiting membrane of the vesicles by the budding process toward the inside of the vesicles. Each of them had an electron-dense, coiled core 128&plusmn;31nm in outside diameter coated by a single unit membrane (envelope). Such knobs as noted by some previous authors were not recognized on their surface.

著者

内川 公人 山田 喜紹 佐藤 潔 熊田 信夫

出版者

日本衛生動物学会

雑誌

衞生動物 (ISSN:04247086)

巻号頁・発行日

vol.34, no.2, 1983-06-15

著者

内川 公人 仲間 秀典 山田 喜紹

出版者

日本衛生動物学会

雑誌

衛生動物 (ISSN:04247086)

巻号頁・発行日

vol.46, no.2, 1995

著者

田波 洋 山田 喜紹 田崎 忠勝 村上 昇

出版者

長野県医学会

雑誌

信州医学雑誌 (ISSN:00373826)

巻号頁・発行日

vol.16, no.1, pp.101-108, 1967-02

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信州医学雑誌 16(1): 101-108(1967)

著者

川森 文彦 熊田 信夫 山田 喜紹

出版者

日本衛生動物学会

雑誌

衞生動物 (ISSN:04247086)

巻号頁・発行日

vol.40, no.3, 1989-09-15