著者

鈴木 三郎

出版者

公益社団法人 日本獣医学会

雑誌

日本獸醫學會雑誌

巻号頁・発行日

vol.6, no.1, pp.52-63, 1927

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Raw meat is the essential substance for the preparation of culture media. The cooked meat extracts in the form of broth contains nitrogenous substances insufficient for the multiplication of the cultivated bacteria. The deficiency is supplimented with an additional pepton.<br>The routine method of the preparation of media is first to obtain the extracts by cooking meat and the broth thus obtained contains only 9% soluble protein, while the remaining 91% insoluble part of the nitrogenous compounds is cast away as rubbish. I tried to find a method by which this cast away rubbish might be of any use for the manufacture of media, economically to facilitate the experimental works in the laboratory. I employed papain, a decomosing ferment of vegetable proteins in my experimental work and the results are dealt with in this communication, which can be summalized as follows:<br>1. 450 gr. of the raw meat was boiled by direct heat with an addition of 0.5 gr. of the powder of papain and obtained 30-55% soluble protein of the total protein contents of the material.<br>2. Various species of bacteria multipli in the medium prepared after my method. Moreover, the colonies in it had almost the equal sizes and the total amount of the bacteria was also almost equal to what was obtained by the use of the Teruuchipepton-broth.<br>3. The production of tetanus toxin in my broth was. almost equal to what was obtained by the use of either the Teruuchipepton-broth or the Hida-pepton-broth.