著者

篠田 雅人 高木 良成 玉置 文一

出版者

公益社団法人 日本薬学会

雑誌

藥學雜誌 (ISSN:00316903)

巻号頁・発行日

vol.86, no.4, pp.306-314, 1966

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Anti-saliva Parotin-A serum was obtained after the administration of saliva Parotin-A (SPA) to intact rabbits in the following doses : 10 μg./kg. for 10 days, 100 μg./kg. on the 11th day, 1 mg. on the 42nd day, and 2 mg. on the 54th day. By the technique of Ouchterlony, the antiserum obtained showed a precipitin band against SPA, but was not reactive to Parotin, S-Parotin, α-Uroparotin, β-Uroparotin, lyophylized saliva, etc., showing immunological specificity of the antiserum. The quantitative precipitin tests indicated that 1 ml. of the antiserum corresponded to 300 μg. of SPA. Administration of SPA mixed with the antiserum was found to be as effective as SPA alone upon the circulating leucocytes, while the antiserum itself showed no significant influence upon them. After mixing SPA with the antiserum in a ratio which ensured complete precipitation of SPA, the precipitate as well as the supernatant showed leucocyte-promoting activitiy. Besides this activity, the precipitate reduced the circulating lymphocytes, while the supernatant increased the granulocytes more markedly. These results suggest that the SPA used in this study contained at least two components ; one was precipitated by the antiserum and, after precipitation, still retained its leucocyte-promoting activity and the other was not precipitated. It was also found that the antigenic center of SPA would be different from the leucocyte-promoting activity in the molecules.