- 著者
-
藤井 優寿
宮地 夏海
常松 順子
中牟田 啓子
- 出版者
- 日本食品化学学会
- 雑誌
- 日本食品化学学会誌 (ISSN:13412094)
- 巻号頁・発行日
- vol.25, no.2, pp.92-96, 2018
A method for analysis of okadaic acid (OA), dinophysistoxin-1 (DTX1) and dinophysistoxin-2 (DTX2) using deproteinationcartridge filter was developed. This is a simple method because it does not require complicated operations such as conditioningand washing of the column. The shellfish poisons in oyster were extracted with methanol and methanol/water (9:1), and werehydrolyzed with 2.5 mol/L sodium hydroxide solution. The crude extracts were defatted by liquid- liquid partition using n-hexane,and were passed through a deproteination cartridge filter. Measurement was performed by liquid chromatography tandem massspectrometry. As a result of the validation study using the matrix calibration curve method, the trueness, repeatability and withinlaboratoryreproducibility were 92-98, 1.0-3.0 and 2.0-4.0%, respectively, and met the target values of notification of Ministry ofHealth, Labour and Welfare of Japan.