著者

藤井 優寿 宮地 夏海 常松 順子 中牟田 啓子

出版者

日本食品化学学会

雑誌

日本食品化学学会誌 (ISSN:13412094)

巻号頁・発行日

vol.25, no.2, pp.92-96, 2018

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A method for analysis of okadaic acid (OA), dinophysistoxin-1 (DTX1) and dinophysistoxin-2 (DTX2) using deproteinationcartridge filter was developed. This is a simple method because it does not require complicated operations such as conditioningand washing of the column. The shellfish poisons in oyster were extracted with methanol and methanol/water (9:1), and werehydrolyzed with 2.5 mol/L sodium hydroxide solution. The crude extracts were defatted by liquid- liquid partition using n-hexane,and were passed through a deproteination cartridge filter. Measurement was performed by liquid chromatography tandem massspectrometry. As a result of the validation study using the matrix calibration curve method, the trueness, repeatability and withinlaboratoryreproducibility were 92-98, 1.0-3.0 and 2.0-4.0%, respectively, and met the target values of notification of Ministry ofHealth, Labour and Welfare of Japan.