著者

藤野 正行 何 普明

出版者

Japanese Society for Food Science and Technology

雑誌

日本食品科学工学会誌 : Nippon shokuhin kagaku kogaku kaishi = Journal of the Japanese Society for Food Science and Technology (ISSN:1341027X)

巻号頁・発行日

vol.45, no.10, pp.618-623, 1998-10-15

参考文献数

17

被引用文献数

8 5

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食用キノコであるタモギタケの加工にともなって生じる煮汁の有効利用に端を発した研究の一環として,熱水抽出物(煮汁)の血糖値抑制効果を調べた.<BR>タモギタケの熱水抽出物は,経口投与により,II型糖尿病モデルマウスKK-A<SUP>y</SUP>の血糖値上昇を抑制し,耐糖能を改善した.<BR>熱水抽出物をβ-グルカナーゼ処理した後,3倍容のエタノールで処理して得たエタノール処理画分は,KK-A<SUP>y</SUP>マウスの血糖値を一時的に抑制したが,作用は微弱であった.<BR>熱水抽出物を対照動物(C57BL/6Jマウス)に投与したが,血糖値および耐糖能に変化はみられなかった.<BR>今後,有効成分の特定と作用機序の解明が必要であるが,本研究は,副生物の有効利用の可能性を示唆した.

著者

河岸 洋和 畠山 美奈 何 普明 杉山 公男

出版者

天然有機化合物討論会

雑誌

天然有機化合物討論会講演要旨集

巻号頁・発行日

no.42, pp.517-522, 2000-10-01

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In the course of screening for liver injury preventing activity of 22 fruits, we found strong activity of avocado. We describe the isolation, structure determination, and biological activity of the liver injury preventing substances from the fruit Lyophilized materials were extracted with hexane. The hexane extract was chromatographed on a silica gel column followed by HPLC using an ODS column, giving five compounds 1-5. FAB-MS (positive) of 1 showed the [M+H]^+ ion at m/z 351. Its ^<13>C-NMR and DEPT experiment exhibited the presence of a carbonyl group (δ189.2), 4 double bonds (δ126.3, 127.8, 128.3, 129.2, 129.8, 130.3, 144.8, 148.7), 11 methylene groups, and a methyl one (δ14.09). Finally, the structure of the compound was determined by the interpretation of HMBC spectrum and its EI-MS fragmentation. Compounds 2 and 3 have been already isolated as an antifungal substance and/or radical scavengers. Elucidation of the structures of compounds 4 and 5 is now in progress. In vivo assay was done as follows. After direct administration of each compound (100mg/kg weight) into rats' stomachs by using catheters, liver injury was induced by treatment of the rats with D-GalN. All the compounds suppressed the increase of ALT and AST activity in the serum of the rats.