著者
崔 宰熏 河岸 洋和
出版者
公益社団法人 日本農芸化学会
雑誌
化学と生物 (ISSN:0453073X)
巻号頁・発行日
vol.49, no.5, pp.299-301, 2011-05-01 (Released:2012-04-27)
参考文献数
13
被引用文献数
1
著者
崔 宰熏 河岸 洋和
出版者
植物化学調節学会
雑誌
植物の生長調節 (ISSN:13465406)
巻号頁・発行日
vol.47, no.1, pp.34-39, 2012-05-31

Rings or arcs of fungus-stimulated plant growth occur often on the floor of woodlands which are commonly called "fairy rings". The fairy-ring forming fungi cause circles of mushrooms, rings or arcs of dark green grass due to luxuriant growth, or rings of dead grass. The liquid-cultured fungus was filtered, the filtrate was fractionated by successive chromatography, and the fractions were tested for their growth regulating activity on bentgrass. This lead to the purification of the active principles, 2-azahypoxanthine (AHX) and imidazole-4-carboxamide (ICA). Both AHX and ICA increased rice seed yield by 25.5% and 26.0%, respectively, in a greenhouse experiment. The growth-promoting activity of AHX was investigated using a rice oligo DNA microarray, reverse transcriptase-polymerase chain reaction, and bioassay. The results indicate that plants develop tolerance to various and continuous stress from the environment and increased nitrogen absorption by AHX-treatment, resulting in the growth promotion.
著者
鈴木 智大 小川 哲弘 阿部 暢男 赤地 拓澄 増田 貴久子 小山 智之 矢澤 一良 河岸 洋和
出版者
天然有機化合物討論会実行委員会
雑誌
天然有機化合物討論会講演要旨集
巻号頁・発行日
vol.49, pp.383-388, 2007

Osteoporosis is caused by an imbalance between bone resorption and bone formation, which results in bone loss and fractures after mineral flux. Osteoclast-like multinucleated cells can be differentiated in vitro from co-cultures of mouse bone marrow cells and osteoblastic cells by treatment with osteotropic factors, 1α,25-dihydroxyvitamin D3 (1α,25(OH)_2D_3) and prostaglandin E2 (PGE2). During screening for osteoclast-formation suppressing effects of the extracts of various mushrooms by using the assay, we found very strong activity in the extract of the mushroom Agrocybe chaxingu. Therefore, an attempt was made to isolate the active principles from mushroom and to determine their structures. Powder of the dried fruiting bodies of Agrocybe chaxingu was extracted with CH_2Cl_2, EtOAc and then EtOH. The CH_2Cl_2-soluble fraction only showed the suppressing activity. After repeated chromatography of the fraction, compounds 1 and 2 were purified as the active principles. Osteoclast differentiation was estimated by TRAP-(+) multinucleated cell formation. The addition of compound 1and 2 (3.1μg/ml, 6.8mM) reduced the number of TRAP-(+) multinucleated cells to 66% and 0%, respectively.
著者
小川 哲弘 赤地 拓澄 増田 貴久子 山口 宏二 矢澤 一良 高橋 守 河岸 洋和
出版者
日本菌学会
雑誌
日本菌学会大会講演要旨集
巻号頁・発行日
vol.50, pp.106, 2006

In recent years, the number of osteoporosis patients is increasing. Various foods were screened for inhibition activity of osteoclast formation and we found that <I>Agrocybe chaxingu</I> showed potent inhibition activity. In this research, we tried to purify the active principle(s) from <I>Agrocybe chaxingu</I>.Powder of dried fruiting bodies of the mushroom was extract with CH<SUB>2</SUB>Cl<SUB>2</SUB>, followed by EtOAc and EtOH. After removing each solvent, each fraction was tested for the inhibition activity. Scince the CH<SUB>2</SUB>Cl<SUB>2</SUB> soluble part showed the strongest inhibition activity, the fraction was fractionated by flash column chromatography, preparative TLC, and HPLC with a C30 column. As a result, we obtained an active compound (8.1 mg) and determined its structure. This compound strongly inhibited osteoclast formation without no cytotoxicity.
著者
杉浦 立樹 山岸 賢治 平井 浩文 河岸 洋和
出版者
一般社団法人 日本木材学会
雑誌
木材学会誌 (ISSN:00214795)
巻号頁・発行日
vol.56, no.6, pp.382-387, 2010-11-25 (Released:2011-03-05)
参考文献数
15
被引用文献数
2 2

高活性リグニン分解菌Phanerochaete sordida YK-624株の産生する,新規リグニンペルオキシダーゼの1種であるYK-LiP2をコードする遺伝子ylpAを高発現する形質転換体A-11株のリグニン分解特性を調査した。ブナ木粉培地においてA-11株は野生株より高いリグニン分解活性を示し,4週間培養後のリグニン分解率は野生株と比較して7.6%高い値を示した。また,その時のリグニン分解選択性も野生株より高い値を示した。A-11株を接種した木粉中のLiP活性は野生株のものより高く,また組換えylpAの転写解析より,A-11株は培養期間を通して安定して組換えylpAを転写していることが判明した。これらの結果より,ylpAの高発現がP.sordida YK-624株のリグニン分解活性を上昇させたことが示された。さらに,市販のセルラーゼを用いた酵素糖化性について検討したところ,A-11株により処理した木粉は野生株のものより高い糖化性を示した。
著者
鈴木 智大 川口 卓巳 天野 裕子 小林 夕香 森田 達也 長井 薫 新井 信隆 河岸 洋和
出版者
天然有機化合物討論会
雑誌
天然有機化合物討論会講演要旨集
巻号頁・発行日
no.48, pp.325-330, 2006-09-15

In autumn, 2004, 55 people got poisoned by eating an edible wild mushroom, Pleurocybella porrigens (Sugihiratake in Japanese) and 17 people among them died of acute encephalopathy. We found that mice died by injection of the wter-soluble extracts of the mushroom. We tried the isolation of the toxic principle(s) from the extracts. As a result, a lectin (PPL) and a glycoprotein were purified from the mushroom. PPL is a tetramer of identical subunits without S-S linkage. The lectin specifically bound N-acetylgalactosamine among monosacchrides tested. This lectin showed lethal toxicity against rats. In addition, a glycoprotein was isolated as a lethal toxin against mice and rats. The relation between these substances and acute encephalopathy is now being elucidated.
著者
河岸 洋和 畠山 美奈 何 普明 杉山 公男
出版者
天然有機化合物討論会
雑誌
天然有機化合物討論会講演要旨集
巻号頁・発行日
no.42, pp.517-522, 2000-10-01

In the course of screening for liver injury preventing activity of 22 fruits, we found strong activity of avocado. We describe the isolation, structure determination, and biological activity of the liver injury preventing substances from the fruit Lyophilized materials were extracted with hexane. The hexane extract was chromatographed on a silica gel column followed by HPLC using an ODS column, giving five compounds 1-5. FAB-MS (positive) of 1 showed the [M+H]^+ ion at m/z 351. Its ^<13>C-NMR and DEPT experiment exhibited the presence of a carbonyl group (δ189.2), 4 double bonds (δ126.3, 127.8, 128.3, 129.2, 129.8, 130.3, 144.8, 148.7), 11 methylene groups, and a methyl one (δ14.09). Finally, the structure of the compound was determined by the interpretation of HMBC spectrum and its EI-MS fragmentation. Compounds 2 and 3 have been already isolated as an antifungal substance and/or radical scavengers. Elucidation of the structures of compounds 4 and 5 is now in progress. In vivo assay was done as follows. After direct administration of each compound (100mg/kg weight) into rats' stomachs by using catheters, liver injury was induced by treatment of the rats with D-GalN. All the compounds suppressed the increase of ALT and AST activity in the serum of the rats.
著者
河岸 洋和
出版者
社団法人日本農芸化学会
雑誌
日本農藝化學會誌 (ISSN:00021407)
巻号頁・発行日
vol.68, no.12, pp.1671-1677, 1994-12-01
被引用文献数
3 2