著者

八木 美智子 山口 虎雄

出版者

Japanese Electrophoresis Society

雑誌

生物物理化学 (ISSN:00319082)

巻号頁・発行日

vol.42, no.1, pp.35-47, 1998-02-15 (Released:2009-03-31)

参考文献数

23

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We investigated the increase in body mass and growth of tumors in rat exposed to negative and positive corona discharges (e-- and e+-ions) and high voltage electric fields (HVE) to determine any influence on body mass and tumor growth of electric fields, voltage, currents and electro magnetic fields. Tumor growth was evaluated by histological findings and analysis of the logistic curves (growth curve) determining the stabilized initial size of tumors (10cm3) corresponding to the synchronization of cultured cells. The histology of benzo (a) pyrene (BP)-stimulated tumor cells showed fibrosarcoma cells with abundant progressive pleomorphism and atypical cells in the control rats, and fibroblastic tumor cells with histiocytic and apoptotic cells with loss of pleomorphic and atypical cells in both e--ion and e+-ion and HVE groups. The logistic curves (growth curve) of tumor growth were represented by Hill's components, and reflected histological features. The tumor growth curve has two or three inflection points (x'') on the growth curve. Exposure of rats to both negative and positive corona discharges delayed tumor growth. While, body mass and tumor were activated by negative corona discharge (e--ion) and they grew 1.5 to 1.7-fold (body mass) and 1.2-fold (tumor) greater than in the respective control groups, finally. The effects of e--ion on growth may be via an induced chronic depolarizing response. Tumors in the e--ion group were cylindrical, extending toward the head, and these animals showed internal hemorrhaging after long term exposure. Rats exposed to the positive corona discharge (e+-ion) showed decreased body and tumor growth and survival. The circular tumors in the e+-ion group contained fibroblastic cells and a few apoptotic cells. These effects of e+-ion were thought to be due to a chronic hyperpolarizing response. Tumor growth under high voltage (HVE) showed a positive cooperative response (burst type cooperativity), which triggered at an early stage of tumor growth, and all internal organs merged mutually due to oppression by HVE. Therefore, the effects of electric fields, currents and high voltages, appear to be a stimulation of growth in the short term, but over the long term serious effects on health were seen.