著者

河合 忠

出版者

Japanese Electrophoresis Society

雑誌

生物物理化学 (ISSN:00319082)

巻号頁・発行日

vol.48, no.3, pp.81-84, 2004-09-15 (Released:2009-03-31)

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Protein science has been advanced with new development of various separation and analytical techniques, and one of the most useful techniques at the beginning was various electrophoretic fractionation. In 1973, Watson & Crick reported on a structure of deoxyribonucleic acid, and then molecular biology has progressed rapidly. In 1991, the international cooperative project on human genome started, and ended in 2003, reporting more than 99% of the whole base sequence of human genome. However, the project has left most of human genes functionally unknown. Currently the major research in life science is aiming to determine biological functions of individual proteins as well as regulatory networks among many proteins, namely “proteomics”.

著者

伊藤 喜久

出版者

Japanese Electrophoresis Society

雑誌

生物物理化学 (ISSN:00319082)

巻号頁・発行日

vol.49, no.2, pp.31-35, 2005-06-15 (Released:2009-03-31)

参考文献数

8

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Urine should be a most valuable routine sample in clinical laboratory. Laboratory data in urine are, however, more or less influenced by many known or unknown, pre-renal, renal and post-renal factors, thus giving the measured value complex and equivocal. In relevance with physicochemical properties and structure of individual proteins, the present author reviewed the factors and their effects on laboratory data in the framework of quality assurance system. Taking properties of individual proteins into considerations we must need to strengthen their clinical significance continuously by investigating and eliminating what are affected by the participating factors.

著者

酒井 栄一 田中 勉 森 光子 中川原 寛一

出版者

Japanese Electrophoresis Society

雑誌

生物物理化学 (ISSN:00319082)

巻号頁・発行日

vol.45, no.1, pp.11-16, 2001-03-15 (Released:2009-03-31)

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If I classify roughly for the quantification method of mRNA, there are the following 5 kinds. 1. Northern and dot hybridization, 2. RNase protection assay, 3. RT-PCR (the use of internal control), 4. competitive RT-PCR (the use of competitor), 5. real time monitoring PCR. In these methods, 3-5 employ PCR. Though 3, 4 are a method to quantify at an exponential increase term, it is different point that 5 is quantification method by means of PCR cycle number to exceed a detection limit of PCR product, just before entering an exponential increase term. Recently, a quantification method by the real time monitoring PCR basks in attention. Not only this method isn't necessary to confirm a cycle number of an exponential increase term in advance but have the wide quantification range in comparison to the method to quantify at an exponential increase term, there are many merits. I introduce LightCyclerTM system (Roche Diagnostics) with this draft as an equipment to be able to do a realtime monitoring.

著者

島尾 和男

出版者

Japanese Electrophoresis Society

雑誌

生物物理化学 (ISSN:00319082)

巻号頁・発行日

vol.41, no.1, pp.1-11, 1997-02-15 (Released:2009-03-31)

被引用文献数

1 3

著者

Zhiwei Qiao Tadashi Kondo

出版者

Japanese Electrophoresis Society

雑誌

Journal of Electrophoresis (ISSN:13499394)

巻号頁・発行日

vol.63, no.1, pp.1-7, 2019 (Released:2019-02-06)

参考文献数

34

被引用文献数

2

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There is a need for novel drugs for sarcoma treatment. In the present study, to identify inhibitors with potential therapeutic utility in sarcomas, we screened the growth inhibitory effects of 361 inhibitors, including experimental reagents and anti-cancer drugs approved for use in non-sarcoma malignancies and those under clinical trials. The inhibitors were initially tested using 10 osteosarcoma cell lines. The half-maximal inhibitory concentration (IC50) of leptomycin B, actinomycin D, chetomin, and staurosporine was <100 nM in all the cell lines. As the promiscuous effects of staurosporine on kinases make it unsuitable for clinical applications, the other three inhibitors were tested in an additional 15 sarcoma cell lines derived from synovial sarcoma, fibrosarcoma, liposarcoma, rhabdomyosarcoma, malignant peripheral nerve sheath tumor, leiomyosarcoma, and Ewing’s sarcoma. The IC50 of leptomycin B and actinomycin D was <100 nM in all cell lines and that of chetomin was <100 nM in all but three synovial sarcoma cell lines. Although the clinical development of leptomycin B, a chromosomal region maintenance (CRM)1/exportin (XPO)1 inhibitor, was discontinued because of toxicity, a previous clinical trial revealed that other CRM1/XPO1 inhibitors, such as selinexor, have anti-tumor effects in sarcomas. Actinomycin D has proven clinical utility in the treatment of sarcomas. Chetomin disrupts the interaction of hypoxia-inducible factor-1 with the transcriptional coactivator p300 and its clinical utility has not been established in sarcomas. Chetomin exhibited growth inhibitory effects on sarcoma cells with different histological subtypes. Library screening is a powerful approach to detect the potential utility of anti-cancer drugs in sarcoma treatment.

著者

鈴木 潤

出版者

Japanese Electrophoresis Society

雑誌

生物物理化学 (ISSN:00319082)

巻号頁・発行日

vol.50, no.2, pp.67-70, 2006-06-15 (Released:2009-03-31)

参考文献数

10

被引用文献数

1 1

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The microbial environment is an essential aspect of the discussion of“genvironment and health”, due to our close coexistence with microorganisms. Furthermore, on a daily basis, bacteria cause food poisoning in humans through food intake, with as many as 90% of food poisoning cases being of bacterial origin. In addition, the Food Hygiene Law classifies etiological agents responsible for food poisoning into 16 types, many of which produce hemolytic toxins as pathogenic factors. Since these toxins are protein toxins, their properties can be determined by electrophoresis analysis. The toxin properties have also been analyzed by conducting osmotic protection and hemolysis inhibition experiments, as well as through determination of their ultrastructure and hemolytic efficiency. Hemolytic efficiency is expressed numerically as a function of streptolysin O (SLO) concentration and hemolysis level, or in other words, the concentration dependency of SLO from the lowest hemolysis level to complete hemolysis. These findings will contribute to the treatment and prevention of food poisoning.

著者

小泉 昭夫

出版者

Japanese Electrophoresis Society

雑誌

生物物理化学 (ISSN:00319082)

巻号頁・発行日

vol.41, no.3, pp.145-148, 1997-06-15 (Released:2009-03-31)

参考文献数

22

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遺伝的に肥満を発症するob/obとdb/dbマウスの発見は, 肥満の分子生物学的検討を可能にし, レプチンとその受容体の発見へと導いた. レプチンと, レプチン受容体, および関連するシグナル介在物質は, ネットワークを形成し, 体脂肪量をある先見的に決められたセットポイントに保つように協調している. レプチンはシグナルとして脂肪組織から大脳へ情報を伝達し, エネルギーの摂取と消費のバランスを取っている. エネルギー摂取の制御は食欲を介し, 消費の制御は熱産生を制御することで行っている. レプチンにより肥満が解消できるのはほんのわずかの人々であると予想されるが, この発見は抗肥満薬として多くの人々に希望を与えており, この驚異のやせ薬は, 近年現実のものになりつつある.

著者

舩渡 忠男 藤巻 慎一 佐藤 淳子 小澤 鹿子 賀来 満夫

出版者

Japanese Electrophoresis Society

雑誌

生物物理化学 (ISSN:00319082)

巻号頁・発行日

vol.45, no.2, pp.149-152, 2001-06-15 (Released:2009-03-31)

参考文献数

8

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Rapid advances in the field of molecular genetics have led to the need for a analytical assay. A number of methods have devised for this purpose, a material used the most widely is used the leukocytes DNA. In this study, we have reported the cases of three types. First, we found the cases who were heterozygotes for adenine phosphoribosyltransferase (APRT) gene mutations with APRT deficiency. Second, we evaluated the usefulness of a real-time reversed transcriptase polymerase chain reaction (RTPCR) system to detect minimal residual diseases. This method was applied a case who had t (8; 21) chimeric transcript in peripheral blood. Third, we evaluated a method to determine human cytomegalovirus (CMV) DNA levels in blood cells in a case transplaned liver. Thus, these methods were indicated possible applications for analysis using blood leukocytes.

著者

八木 美智子 山口 虎雄

出版者

Japanese Electrophoresis Society

雑誌

生物物理化学 (ISSN:00319082)

巻号頁・発行日

vol.42, no.1, pp.35-47, 1998-02-15 (Released:2009-03-31)

参考文献数

23

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We investigated the increase in body mass and growth of tumors in rat exposed to negative and positive corona discharges (e-- and e+-ions) and high voltage electric fields (HVE) to determine any influence on body mass and tumor growth of electric fields, voltage, currents and electro magnetic fields. Tumor growth was evaluated by histological findings and analysis of the logistic curves (growth curve) determining the stabilized initial size of tumors (10cm3) corresponding to the synchronization of cultured cells. The histology of benzo (a) pyrene (BP)-stimulated tumor cells showed fibrosarcoma cells with abundant progressive pleomorphism and atypical cells in the control rats, and fibroblastic tumor cells with histiocytic and apoptotic cells with loss of pleomorphic and atypical cells in both e--ion and e+-ion and HVE groups. The logistic curves (growth curve) of tumor growth were represented by Hill's components, and reflected histological features. The tumor growth curve has two or three inflection points (x'') on the growth curve. Exposure of rats to both negative and positive corona discharges delayed tumor growth. While, body mass and tumor were activated by negative corona discharge (e--ion) and they grew 1.5 to 1.7-fold (body mass) and 1.2-fold (tumor) greater than in the respective control groups, finally. The effects of e--ion on growth may be via an induced chronic depolarizing response. Tumors in the e--ion group were cylindrical, extending toward the head, and these animals showed internal hemorrhaging after long term exposure. Rats exposed to the positive corona discharge (e+-ion) showed decreased body and tumor growth and survival. The circular tumors in the e+-ion group contained fibroblastic cells and a few apoptotic cells. These effects of e+-ion were thought to be due to a chronic hyperpolarizing response. Tumor growth under high voltage (HVE) showed a positive cooperative response (burst type cooperativity), which triggered at an early stage of tumor growth, and all internal organs merged mutually due to oppression by HVE. Therefore, the effects of electric fields, currents and high voltages, appear to be a stimulation of growth in the short term, but over the long term serious effects on health were seen.

著者

小笠原 至玄 山崎 仁 布村 渉

出版者

Japanese Electrophoresis Society

雑誌

生物物理化学 (ISSN:00319082)

巻号頁・発行日

vol.31, no.3, pp.155-158, 1987-06-15 (Released:2009-03-31)

参考文献数

7

被引用文献数

2 3

著者

原田 勝二

出版者

Japanese Electrophoresis Society

雑誌

生物物理化学 (ISSN:00319082)

巻号頁・発行日

vol.41, no.3, pp.111-116, 1997-06-15 (Released:2009-03-31)

参考文献数

17

著者

川口 竜二 竹村 一男 川俣 治 小笠 詩子

出版者

Japanese Electrophoresis Society

雑誌

生物物理化学 (ISSN:00319082)

巻号頁・発行日

vol.40, no.6, pp.299-303, 1996-12-15 (Released:2009-03-31)

著者

松尾 雄志

出版者

Japanese Electrophoresis Society

雑誌

生物物理化学 (ISSN:00319082)

巻号頁・発行日

vol.50, no.1, pp.9-11, 2006-03-15 (Released:2009-03-31)

著者

宮地 隆興 大庭 雄三

出版者

Japanese Electrophoresis Society

雑誌

生物物理化学 (ISSN:00319082)

巻号頁・発行日

vol.23, no.2, pp.175-178, 1979-09-25 (Released:2009-03-31)

参考文献数

15

著者

井上 勤 浅賀 宏昭 田村 真弓

出版者

Japanese Electrophoresis Society

雑誌

生物物理化学 (ISSN:00319082)

巻号頁・発行日

vol.30, no.3, pp.229-238, 1986-05-31 (Released:2009-03-31)

参考文献数

19

被引用文献数

1 1

著者

縄田 修吾 加藤 紘 中村 和行

出版者

Japanese Electrophoresis Society

雑誌

生物物理化学 (ISSN:00319082)

巻号頁・発行日

vol.42, no.4, pp.257-263, 1998-12-15 (Released:2009-03-31)

参考文献数

10

被引用文献数

1

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Recently, tumor markers, which have been developed for the diagnosis and evaluation for cancer, are regarded as new key tools to study the malignant behavior of cancer, and therefore, much attension has been focused on their biological functions. Squamous cell carcinoma (SCC) antigen is a tumor marker of SCC arising in various sites. We have investigated the biological function and heterogeneity of this tumor marker by electrophoretic methods. In this paper, we report that SCC antigen belongs to the inhibitory type of serine protease inhibitor family, indicating that SCC antigen may affect malignant behavior such as tumor invasion. We also describe the different pattern of SCC antigen by two-dimensional electrophoresis in normal and cancer tissues, and indicate that the heterogeneity of SCC antigen results mainly from the presence of two homologous genes, SCCA 1 and SCCA 2 gene.