著者

山本 条太郎 北村 朗 金城 政孝

出版者

一般社団法人 日本生物物理学会

雑誌

生物物理 (ISSN:05824052)

巻号頁・発行日

vol.59, no.3, pp.125-131, 2019 (Released:2019-05-25)

参考文献数

34

被引用文献数

1

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Fluorescence correlation spectroscopy (FCS) is a potential tool to measure the dynamics of fluorescent molecules, either in solution or in a cell. FCS can quantify the diffusion coefficient, the target molecule concentration, and brightness of a single molecule. These parameters allow estimations of the molecular size, the molecular shape, and the affinities of molecular interactions. Moreover, varieties of FCS methods have been developed on the concept of the fluorescence lifetime, triplet state, decrease of fluorescent intensity and toward two-dimensional imaging, in order to reveal the molecular interaction in live cell and molecular crowding.