著者
藤田 克昌
出版者
一般社団法人 日本生物物理学会
雑誌
生物物理 (ISSN:05824052)
巻号頁・発行日
vol.50, no.4, pp.174-179, 2010 (Released:2010-07-25)
参考文献数
23
被引用文献数
2 1 3

Recent developments in fluorescence microscopy techniques have broken the diffraction limit and achieved the spatial resolution of sub 100 nm range. Saturated excitation (SAX) microscopy and stimulated emission depletion (STED) microscopy utilize saturable optical phenomena seen in laser excitation and stimulate emission of fluorescence molecules to induce strongly nonlinear optical effects for the resolution improvement. Photoactivated localization microscopy (PALM) and stochastic optical reconstruction microscopy (STORM) applied photoswitchable fluorescence probes for precise measurement of positions of the fluorescence probes in a sample in a few tens of nanometer scale. This review introduces the principles and the characteristics of those super resolution microscopy techniques with discussing the imaging formation and the resolution limit in conventional microscopy techniques.
著者
伏見 圭司 成川 礼
出版者
一般社団法人 日本生物物理学会
雑誌
生物物理 (ISSN:05824052)
巻号頁・発行日
vol.58, no.6, pp.303-307, 2018 (Released:2018-11-27)
参考文献数
20

Because light is not only energy source but also important signal for photosynthetic organisms, these organisms develop highly organized light acclimation processes. Linear tetrapyrrole-binding photoreceptors play central roles in these processes. They are categorized into phytochrome and cyanobacteriochrome families. Here, we summarize current knowledge on these photoreceptor families especially focusing on color-tuning mechanisms of the cyanobacteriochromes. Because these photoreceptors possess many advantages for optogenetic and bio-imaging applications, we briefly introduce current developmental status of these photoreceptors.
著者
鈴木 誉保
出版者
一般社団法人 日本生物物理学会
雑誌
生物物理 (ISSN:05824052)
巻号頁・発行日
vol.61, no.1, pp.031-035, 2021 (Released:2021-01-29)
参考文献数
10
著者
宮田 真人
出版者
一般社団法人 日本生物物理学会
雑誌
生物物理 (ISSN:05824052)
巻号頁・発行日
vol.60, no.4, pp.231-235, 2020 (Released:2020-07-29)
参考文献数
11
被引用文献数
2

Motility is a major characteristic of living things. We categorized all motilities reported so far based on the structure of the force-generating unit, and revealed that they can be classified into 18 types. Furthermore, we have proposed the origin and evolution of these 18 motilities on a phylogenetic tree constructed from new genomic information.
著者
田浦 太志 正山 祥生 森元 聡
出版者
一般社団法人 日本生物物理学会
雑誌
生物物理 (ISSN:05824052)
巻号頁・発行日
vol.45, no.4, pp.178-184, 2005 (Released:2005-07-25)
参考文献数
10
被引用文献数
2 2

Cannabis sativa produces novel secondary metabolites called cannabinoids. Among them, Δ1-tetrahydrocannabinol (THC), the psychoactive component of this plant, has attracted a great deal of attention, because this cannabinoid is shown to exert a variety of therapeutic activities such as the relief of nausea caused by cancer chemotherapy. To reveal the mechanism of THC formation in Cannabis sativa, we attempted biosynthetic studies. Consequently, we established that Δ1-tetrahydrocannabinolic acid (THCA), the precursor of THC, is derived from cannabigerolic acid and that this reaction is catalyzed by the novel oxidocyclase, THCA synthase. Here, we show the biochemical and structural properties of THCA synthase.