著者
藤田 克昌
出版者
一般社団法人 日本生物物理学会
雑誌
生物物理 (ISSN:05824052)
巻号頁・発行日
vol.50, no.4, pp.174-179, 2010 (Released:2010-07-25)
参考文献数
23
被引用文献数
2 1 3

Recent developments in fluorescence microscopy techniques have broken the diffraction limit and achieved the spatial resolution of sub 100 nm range. Saturated excitation (SAX) microscopy and stimulated emission depletion (STED) microscopy utilize saturable optical phenomena seen in laser excitation and stimulate emission of fluorescence molecules to induce strongly nonlinear optical effects for the resolution improvement. Photoactivated localization microscopy (PALM) and stochastic optical reconstruction microscopy (STORM) applied photoswitchable fluorescence probes for precise measurement of positions of the fluorescence probes in a sample in a few tens of nanometer scale. This review introduces the principles and the characteristics of those super resolution microscopy techniques with discussing the imaging formation and the resolution limit in conventional microscopy techniques.

17 0 0 0 OA 若手の声

出版者
一般社団法人 日本生物物理学会
雑誌
生物物理 (ISSN:05824052)
巻号頁・発行日
vol.53, no.3, pp.173-174, 2013 (Released:2013-05-29)
著者
福島 健児 藤田 浩徳
出版者
一般社団法人 日本生物物理学会
雑誌
生物物理 (ISSN:05824052)
巻号頁・発行日
vol.56, no.5, pp.255-261, 2016 (Released:2016-09-27)
参考文献数
15

Carnivorous plants produce morphologically complex leaves that serve as a trapping device. Among a variety of the trap leaves, pitcher-shaped leaves have been considered to have evolved by drastic morphological changes, since no evolutionary intermediate has been identified in both extant and extinct species. Here we review roles of organ polarity and cell division patterns in the pitcher development, as well as applications of vertex dynamics model to understand leaf development. Development and evolution of pitcher leaves are inferred from both experimental and computational approaches.
著者
村田 武士
出版者
一般社団法人 日本生物物理学会
雑誌
生物物理 (ISSN:05824052)
巻号頁・発行日
vol.54, no.2, pp.079-084, 2014 (Released:2014-03-28)
参考文献数
28
被引用文献数
1 1

F- and V-ATPases are unique bio- and nano-molecular rotary motors among many types of bioenergy-transducing machineries. The rotational catalysis of F1-ATPase has been investigated in detail, and the molecular mechanisms have been proposed on the basis of crystal structures of the complex and extensive single-molecule observation of the rotation. Recently, we have obtained crystal structures of bacterial V1-ATPase (A3B3 and A3B3DF complexes) with and without nucleotide. On the basis of these new structures, we present a novel model of the rotational catalytic mechanism for V1-ATPase, which is apparently different from those of F1-ATPases.
著者
力丸 裕
出版者
一般社団法人 日本生物物理学会
雑誌
生物物理 (ISSN:05824052)
巻号頁・発行日
vol.49, no.4, pp.174-180, 2009 (Released:2009-07-25)
参考文献数
18
被引用文献数
1

Small bats (microchiroptera) get various types of information about their targeted objects by comparing returning echoes to emitted ultrasonic pulses, which is called “Biosonar”. This paper introduces the outline of biosonar, and then explains consistency and plasticity of vocalization, changes in vocalization due to relationships between targets and the bat. This paper also describes how they vocalize for the real-time time-sharing signal processing based upon data obtained with an onboard telemetry microphone system and data recorded with reversible focal inactivation of neural activities. Amazing real-time time-sharing signal processing performed by a brain as small as the tip of our little finger will be explained.
著者
岩切 淳一 浜田 道昭
出版者
一般社団法人 日本生物物理学会
雑誌
生物物理 (ISSN:05824052)
巻号頁・発行日
vol.56, no.4, pp.217-220, 2016 (Released:2016-07-25)
参考文献数
20

Recent advances in high throughput sequencing technologies unveiled that large number of long non-coding RNAs (lncRNAs) are transcribed from human genome. Currently, these emerging transcripts are needed to be functionally classified and annotated. Here we review several bioinformatic approaches for analyzing the important characteristics of the lncRNAs toward discovering their functions: 1) tissue specificities of lncRNA expressions, 2) two types of macromolecular interactions (RNA-RNA and RNA-protein interactions), 3) secondary structures of lncRNAs.