著者

山本 俊一 長谷川 斐子 呉 大順

出版者

日本細菌学会

雑誌

日本細菌学雑誌 (ISSN:00214930)

巻号頁・発行日

vol.22, no.10, pp.600-605, 1967-10-25 (Released:2009-02-19)

参考文献数

29

---

1) The suspenion of E. coli was heated under the environment of oxygen with from 1 to 10 absolute atmospheric pressure (aap). At each temperature, the decimal reduction time showed a peak at 1aap, and then decreased as the pressure rose.2) When E. coli was cultivated under the hyperbaric oxygen, the lag time was delayed with the increase of pressure up to 4aap. At 5aap or more of oxygen, no bacterial growth was observed. Generation time increased linearly with the pressure of oxygen up to 3aap.3) The pretreatment with hyperbaric oxygen had not effect on the growth rate thereafter.4) When the culture was oxygenated during the growth phase, the following relationship was observed between the number of the microbes (N) and oxygen pressure (p)N=N0e(3.7-0.8p)t(t: time in hour)5) The amount of final growth showed the peak at 1aap. Exceeding 1aap, the amount diminished remarkably with the rise of the pressure.6) Hyperbaric nitrogen showed no effect on the growth of bacteria.7) Reducing agents added to the media had no protecting effect against the oxygen action on E. coli.8) Antimetabolites showed the synergic, antagonistic and indifferent attitude toward the effect of hyperbaric oxygen depending on the mode of action of the chemicals.

著者

山本 俊一 宮崎 正之助 岡田 和夫 館野 功 高木 忠信 古田 昭一 呉 大順 佐藤 富蔵

出版者

日本細菌学会

雑誌

日本細菌学雑誌 (ISSN:00214930)

巻号頁・発行日

vol.20, no.8, pp.534-539, 1965-08-25 (Released:2009-02-19)

参考文献数

18

---

The authors tried to elucidate the therapeutic effect of hyperbaric oxygenation on the experimental infection of the anaerobic pathogens, using mice and rabbits as experimental animals.1) Cl. novyi was rather resistant to the action of oxygen at high pressure. It was presumably due to the spore formed.2) In order to prevent the death of the mice inoculated with Cl. novyi, the animals should be repeatedly exposed to the oxygen at moderate pressure. The excessive oxygenation was toxic to the animals, rendering them less resistant to the infection.3) Toxin of Cl. novyi was not decomposed by hyperbaric oxygenation. It has also no effect on the detoxication process of the organisms or on the toxin neutralization in vitro and in vivo.4) In case of gas gangrene hyperbaric oxygenation therapy should be combined with antitoxin treatment, as the former suppresses the growth of pathogens and the latter neutralizes toxin produced.5) In the experiment with with rabbit, repeated hyperbaric oxygenation was proved markedly effective to the infection with Cl. norvyi, but less effective to Cl. tetani.In the latter case, the excessive oxygenation was unfavorable to the infected animals.