著者

甲斐 雅亮 坂本 靖浩 三浦 哲朗 大倉 洋甫

出版者

公益社団法人 日本分析化学会

雑誌

分析化学 (ISSN:05251931)

巻号頁・発行日

vol.35, no.3, pp.329-332, 1986-03-05 (Released:2009-06-30)

参考文献数

7

被引用文献数

2 2

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A high performance liquid chromatographic method is described for the determination of antipain which is a protease inhibitor and also a possible therapeutic drug for muscular destrophy. Antipain was converted to the fluorescent derivative by reaction with benzoin, a fluorogenic reagent for guanidino group. The derivative was then determined on a reversed phase column with isocratic elution within 15 min by using fluorescence detector. The detection limit of antipain in mouse serum was 0.42μg/ml, which corresponds to the amount of 240 pg (400 fmol) in an injection volume of 100μ1 at a signal to noise ratio of 2. The calibration graph for the compound was linear up to at least a concentration of 30 μg/ml in the serum. The proposed method was simple, rapid and sensitive enough to permit the quantification of antipain in as little as 40 μl of mouse serum dosed with the compound.