著者

城口 克之

出版者

一般社団法人 日本生物物理学会

雑誌

生物物理 (ISSN:05824052)

巻号頁・発行日

vol.53, no.6, pp.290-294, 2013 (Released:2013-11-25)

参考文献数

25

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The term “RNA sequencing (RNA-Seq)” often means the sequencing of cDNA generated from RNA as well as the direct sequencing of RNA. RNA-Seq has become well known as next generation sequencers emerge, particularly because their high throughput nature of data collection makes RNA-Seq a powerful tool for genome-wide gene expression analysis. In this review, I introduce, mainly from a technical point of view, a basic scheme of RNA sequencing, its development, and its application in several interesting studies.