著者

大崎 千尋

出版者

The Kitakanto Medical Society

雑誌

北関東医学 (ISSN:00231908)

巻号頁・発行日

vol.10, no.3, pp.316-328, 1960

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Investigations were made on the P<SUP>32</SUP> incorporation into the phospholipid fractions in thoracic muscle of dragon fly comparing with heart and femoral muscles of rat. The results obtained by the paperchromatographic method of Dawson were as follows : <BR>1) The total phosphatidylserine, phosphatidylethanolamine and phosphatidylcholine content was more than 70% of the total phospholipids in thoracic muscle of dragon fly, but the diphospho-in ositide content was low.<BR>2) The incoporation of P<SUP>32</SUP> into phosphatidylserine reached its maximum three hours after the ingestion of P<SUP>32</SUP>, but those into phosphatidylcholine and two unidentified substances (Rf value of them was about 0.25 in paperchromatography by phenol-NH<SUB>3</SUB>) were gradually increased ouere period of 20 hours. After 20 hours of P<SUP>32</SUP> ingestion, more than 80% of P<SUP>32</SUP> incorporated into total phospholipids were in the fractions of phosphatidylcholine and two unidentified substances referred to above.<BR>3) The composition of phospholipids of heart and femoral muscles of rat after intraperitoneal injection of P<SUP>32</SUP> were almost the same as that of dragon fly. The P<SUP>32</SUP> incorporation into diphospho-inositide and two unidentified substances of heart muscle of rat were greater than that of femoral muscle after 12 hours, while that of phosphatidylserine was the reverse.<BR>The results were discussed as to the significance of phospholipids as an important endogenous substrate of muscles, especially of insect flight muscle.