著者

金高 節子 宮田 勝保 中村 良治

出版者

The Society of Cosmetic Chemists of Japan

雑誌

日本化粧品技術者会誌 (ISSN:03875253)

巻号頁・発行日

vol.24, no.1, pp.5-12, 1990-08-25 (Released:2010-08-06)

参考文献数

11

被引用文献数

4

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This work was concerned with the characterization of nonkeratinous and keratinous proteins of human hair eluted by permanent wave lotion I (PWL-I). Polyacrylamide gel electrophoresis, infrared spectroscopy and amino acid analysis method were applied.From these examinations, the following results were obtained. (1) Nothing was eluted from human hair by hot water (60°C, 3hr treatment), whereas some extracts were obtained using alkaline solution, surfactant solution, or PWL-I (permanent wave lotion I). (2) The mass of the proteins extracted by PWL-1 accounted for about 1% of the total mass of the hair and tended to increase with an increase in pH. (3) It was confirmed that the molecular weights ranged from 5, 000 to 65, 000 by SDS PAGE. (4) The amino acid composition of the PWL-1 extracts was similar to that of colagenous proteins which was unexpected. The composition was diffrent from that of intercuticular material, δ, eluted by formic acid. Therefore, we suspected that the PWL-1 extract was eluted from the δ layer among the cortical cells.In addition, it was clarified that a part of keratinous protein was extracted from hair which was damaged by repeated treatments of PWL or breached by hydrogen peroxide and ammonia water. It is thought to be eluted from the matrix in the cortical cell which is composed of amorphous keratin of high sulfer content.These results will help clarify the mechanism of the elution of intercellar material of humann hair and the mechanism of hair damage by external treatments.