著者
Agus Bahar Rachman 赤澤 隆志 小川 雅廣
出版者
日本食品化学学会
雑誌
日本食品化学学会誌 (ISSN:13412094)
巻号頁・発行日
vol.27, no.1, pp.10-21, 2020

Olive leaf (<i>Olea europaea</i> L.) is an underutilized resource of olive tree but has a high polyphenol content, so it has the potential to be used as a food ingredient. In this study, we investigated the effects of olive leaf water extracts on the physical properties of chicken breast sausage (CBS). Olive leaf water extracts were prepared at 4 (OEx<sub>4</sub>) and 80°C (OEx<sub>80</sub>) from dried olive leaf powder and they were applied to CBS preparation within the concentration range 0.05 to 0.5% (w/w minced meat). OEx<sub>4</sub> had 32% lower polyphenol content than OEx<sub>80</sub>. Water content, expressible water, breaking strength, and viscoelastic properties of CBS with OEx<sub>4</sub> and OEx<sub>80</sub> were measured. The analyses of the sausage physical properties showed that adding 0.1% OEx<sub>4</sub> increased the breaking stress (115%), breaking strain (68%), modulus of elasticity (20%), coefficient of viscosity (23%), and water-holding capacity (9%), and the enhancement effects of OEx<sub>4</sub> were much greater than those of OEx<sub>80</sub>. The gel network of OEx<sub>4</sub>-containing CBSs observed by scanning electron microscopy was finer and denser than that of the control CBS. Chemical analyses of the meat proteins showed that OEx<sub>4</sub> induces polymerization of myofibril proteins, especially myosin, via non-disulfide covalent bridges formed between amino and/ or thiol groups. These results indicate that the application of OEx<sub>4</sub> improves the sausage gel properties by inducing non-disulfide-type protein polymerization. This study supports the feasibility of OEx<sub>4</sub> as a new ingredient to improve the texture of gel products prepared from chicken breast minced meat.
著者
Agus Bahar Rachman 赤澤 隆志 小川 雅廣
出版者
Japanese Society of Food Chemistry
雑誌
日本食品化学学会誌 (ISSN:13412094)
巻号頁・発行日
vol.27, no.1, pp.10-21, 2020 (Released:2020-04-30)
参考文献数
35

Olive leaf (Olea europaea L.) is an underutilized resource of olive tree but has a high polyphenol content, so it has the potential to be used as a food ingredient. In this study, we investigated the effects of olive leaf water extracts on the physical properties of chicken breast sausage (CBS). Olive leaf water extracts were prepared at 4 (OEx4) and 80°C (OEx80) from dried olive leaf powder and they were applied to CBS preparation within the concentration range 0.05 to 0.5% (w/w minced meat). OEx4 had 32% lower polyphenol content than OEx80. Water content, expressible water, breaking strength, and viscoelastic properties of CBS with OEx4 and OEx80 were measured. The analyses of the sausage physical properties showed that adding 0.1% OEx4 increased the breaking stress (115%), breaking strain (68%), modulus of elasticity (20%), coefficient of viscosity (23%), and water-holding capacity (9%), and the enhancement effects of OEx4 were much greater than those of OEx80. The gel network of OEx4-containing CBSs observed by scanning electron microscopy was finer and denser than that of the control CBS. Chemical analyses of the meat proteins showed that OEx4 induces polymerization of myofibril proteins, especially myosin, via non-disulfide covalent bridges formed between amino and/ or thiol groups. These results indicate that the application of OEx4 improves the sausage gel properties by inducing non-disulfide-type protein polymerization. This study supports the feasibility of OEx4 as a new ingredient to improve the texture of gel products prepared from chicken breast minced meat.
著者
小川 雅廣
出版者
香川大学
雑誌
基盤研究(C)
巻号頁・発行日
2012-04-01

老廃牛肉や廃鶏肉などの硬い肉を、肉特有のテクスチャーを損なうことなく軟化させる技術が求められている。本研究では、肉の硬さの原因であるコラーゲン線維を分解できる酵素(CDE)を海洋生物から探し出し、その酵素が肉を軟化できるか検討した。魚介類(ハマチと帆立貝)の内臓と魚類体表に付着した細菌89菌株にCDEを見出した。最も強い活性を示したのはPseudomonas属菌SF10株(ババガレイ体表に付着)とShewanella属菌JS51株(スズキ体表に付着)由来の酵素であった。SF10由来酵素を大量生産し、牛肉塊に添加し4℃で5日間保存したところ、肉は柔らかくなった。