著者

舩渡 忠男 藤巻 慎一 佐藤 淳子 小澤 鹿子 賀来 満夫

出版者

Japanese Electrophoresis Society

雑誌

生物物理化学 (ISSN:00319082)

巻号頁・発行日

vol.45, no.2, pp.149-152, 2001-06-15 (Released:2009-03-31)

参考文献数

8

---

Rapid advances in the field of molecular genetics have led to the need for a analytical assay. A number of methods have devised for this purpose, a material used the most widely is used the leukocytes DNA. In this study, we have reported the cases of three types. First, we found the cases who were heterozygotes for adenine phosphoribosyltransferase (APRT) gene mutations with APRT deficiency. Second, we evaluated the usefulness of a real-time reversed transcriptase polymerase chain reaction (RTPCR) system to detect minimal residual diseases. This method was applied a case who had t (8; 21) chimeric transcript in peripheral blood. Third, we evaluated a method to determine human cytomegalovirus (CMV) DNA levels in blood cells in a case transplaned liver. Thus, these methods were indicated possible applications for analysis using blood leukocytes.