著者

手塚 雅勝 鈴木 弘美 鈴木 康夫 原 征彦 岡田 昌二

出版者

公益社団法人日本薬学会

雑誌

衛生化学 (ISSN:0013273X)

巻号頁・発行日

vol.43, no.5, pp.311-315, 1997-10-31

参考文献数

13

被引用文献数

10

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The effects of catechins obtained from the hot water extract of green tea leaves on two human type-A influenza virus strains of Aichi/2/68 and PR/8/34 were studied. In this study, (-)-epicatechin (EC), (-)-epigallocatechin (EGC), (-)-epicatechin gallate (ECg), (-)-epigallocatechin gallate (EGCg), the crude catechins containing these catechins and (+)-catechin were used. Consequently it was observed that catechins used in this study had an inhibitory effect on the hemolytic activity to red blood cells induced by these two type-A virus strains under acidic conditions (pH 5.1 or 5.4) although they did not have an influence on the agglutination activity to red blood cells induced by the same virus strains. After incubation of (-)-ECg and (-)-EGCg with A/Aichi/2/68 virus, MDCK cells, virus-sensitive cells, were infected with the virus and the ability of virus proliferation was measured in terms of an index of the agglutination activity of the virus to red blood cells. The used two catechins, (-)-ECg and (-)-EGCg, inhibited the virus proliferation at concentrations of 100μg/ml and 50μg/ml, respectively. Furthermore, at a concentration of 2.0 mg/ml these two catechins were found to inhibit the activity of neuraminidase on the surface of the virus membrane by 71.3% and 60.8%, respectively. From the above-mentioned results, it is considered that among the catechins contained in green tea leaves, such two catechins as (-)-ECg and (-)-EGCg inhibit the activity of neuraminidase present on the surface of human influenza virus in order to block the invasion of the influenza virus into virus-sensitive cells.

著者

浮島 美之 秋元 宣子 成田 弘子 石川 雅章 藤井 貴野 木野 正彦 山脇 正樹 岡田 昌二

出版者

公益社団法人日本薬学会

雑誌

衛生化学 (ISSN:0013273X)

巻号頁・発行日

vol.35, no.5, pp.339-346, 1989-10-31

被引用文献数

1

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To identify the species of mushrooms mainly for the distinction of poisonous mushrooms from edible ones, thin layer isoelectric focusing analysis was applied on their water-soluble proteins. 1. The electrophoretic profiles were species-specific. The proteins extracted from the cap and stem in a species gave essentially identical profiles. 2. The profiles of cap proteins of 10 individuals of Akamomitake (Lactarius deliciosus) were indifferent, indicating that the intra-species variation of water-soluble proteins was little. 3. The heat treatment at higher than 60℃, for 10 min, of the water-soluble proteins of Dokutsurutake (Amanita virosa) caused temperature-dependent disappearance of protein bands, while not significant change up to 60℃. 4. The values of pI and relative peak height of isoelectro-focused water-soluble protein bands were numerically expressed. These numerical values were species-specific, indicating that mushroom species might be identified by analyzing the water-soluble proteins with a verification of the tables of these numerical values. 5. The present method was applied for the identification of poisonous Kusaurabenitake (Rhodophyllus rhodopolius) mixed in Urabenihoteishimeji (Rhodophyllus crassipes) which caused a food poisoning incident. The former mushroom was satisfactorily identified by this method.