11 0 0 0 OA 宮城県内の牡蛎養殖海域に流入する河川水中のノロウイルスの定量
- 著者
- 山本 俊夫 島津 智浩 遠藤 善宏 白石 廣行 影山 努
- 出版者
- 公益社団法人 日本水環境学会
- 雑誌
- 水環境学会誌 (ISSN:09168958)
- 巻号頁・発行日
- vol.28, no.8, pp.515-521, 2005-08-10 (Released:2008-01-22)
- 参考文献数
- 25
- 被引用文献数
- 1 1
The concentrations of norovirus (NV) genes in water samples from rivers A, B and C flowing into oyster farms in Miyagi Prefecture, Japan were determined from October 2002 to March 2004. Norovirus genes in 35 specimens collected over 17 months were measured using genogroup 1 (G1) and genogroup 2 (G2) specific real-time quantitative reverse transcription PCR techniques. The respective positive rates of NV genes in rivers A, B and C were 51% (18 of 35), 97% (34 of 35) and 26% (9 of 35). The respective concentrations of the NV G1 gene ranged from 85 - 5.3 × 103 copies, 80 - 9.6 × 104 copies and 85 - 1.7 × 103 copies in 100 ml of water samples from rivers A, B and C. The respective concentrations of the NV G2 gene ranged from 93 - 2.0 × 103 copies, 65 - 1.5 × 105 copies and 72 - 1.2 × 103 copies in 100 ml of the same water sample. These values were high during winter, and low but detectable during summer.