著者

折茂 英生

出版者

日本医科大学医学会

雑誌

日本医科大学医学会雑誌 (ISSN:13498975)

巻号頁・発行日

vol.7, no.2, pp.92-96, 2011 (Released:2011-05-06)

参考文献数

8

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The delivery of genes into cells is a basic procedure in molecular biology. The induction of genes into prokaryotic cells is referred to as transformation, which is a basic method in gene cloning, whereas that into eukaryotic cells is called transfection. Several transfection methods have been reported: physical, chemical, and biological. In this technical note, a transfection method using cationic lipids is described for studies of gene function and the expression of mutant proteins.