著者

吉川 昌江 佐久間 長彦 日比 野剛 池内 玲子 佐藤 貴昭 米山 明彦 岩田 誠司 川口 正展 神谷 吉宣 伊藤 純子 藤浪 隆夫

出版者

一般社団法人 日本動脈硬化学会

雑誌

動脈硬化 (ISSN:03862682)

巻号頁・発行日

vol.22, no.2-3, pp.203-207, 1994-08-01 (Released:2011-09-21)

参考文献数

25

被引用文献数

1 1

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The Oxidative modification of low-density lipoprotein (LDL) could contribute to atherosclerosis as a result of its cytotoxic effect, uptake by the scavenger receptor, and its influence on monocyte and macrophage migration. Ascorbate is an important watersoluble, chain-breaking antioxidant in humans.In this study, we examined the effect of ascorbate on the Cu2+-induced oxidative modification of LDL. LDL was incubated for 24 hours with 2.5μM copper (Cu2+) in phosphate-buffered saline (PBS) in both the presence and absence of ascorbic acid (20μg/ml, 25μg/ml, 30μg/ml, and 35μg/ml). Ascorbate significantly inhibited the oxidative modification of LDL, as indicated by both the decreased electrophoretic mobility and the linoleic acid content. Oxidative modification was prevented in a concentration-dependent manner by the addition of ascorbate.Our data suggests that ascorbate may play an important physiological role in protection against the oxidative modification of LDL.