著者

小林 正義 佐野 隆一 藤井 豊 及川 真一 阿部 隆三 後藤 由夫 山内 祐一

出版者

一般社団法人 日本動脈硬化学会

雑誌

動脈硬化 (ISSN:03862682)

巻号頁・発行日

vol.14, no.5, pp.1155-1160, 1986-12-01 (Released:2011-09-21)

参考文献数

16

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Lipids metabolism during prolonged fasting (10-days complete fasting) has been studied in 23 psychosomatic patients (9 male and 14 female, average 29.2 y. o.), who had no metabolic disease.During the fasting period, serum F.F.A. and Cholesterol levels significantly elevated (p<0.001).In the lipoprotein fraction, LDL-cholesterol and LDL-triglyceride (d: 1.006-1.063) significantly elevated by fasting (p<0.001), but VLDL-triglyceri de (d;<1.006) decreased (p<0.05).The increase of LDL reflected the increase of LDL2-fraction (d; 1.019-1.063), and this was related to thyroid hormone levels. T3 levels decreased during prolonged fasting (p<0.01).It was considered that increase of LDL was related to the decrease of LDL-receptor activity which was dependent on T3-levels.

著者

米満 吉和 居石 克夫

出版者

一般社団法人 日本動脈硬化学会

雑誌

動脈硬化 (ISSN:03862682)

巻号頁・発行日

vol.27, no.6, pp.179-187, 2000-04-20 (Released:2011-09-21)

参考文献数

48

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Virus infection as a pathogen in human vascular diseases has been an important and unsolved issue to be studied. According to Koch's postulates, several conditions should be met to define an organism as a pathogen of human disease: especially, 1) to confirm the whole or a part of structure of microorganism including genomic DNA or RNA in the human material, 2) whether infection of microorganism can induce similar disease in mammals. Several bacterias such as Chlamydia pneumoniae or Hericobactor pylori have been studied according to these postulates, however, it has been hazardous for virus research to establish animal model due to species-specificity or tropism. Human cytomegalovirus (HCMV) is one of these candidates, and HCMV DNA has been frequently detected in the both normal and disordered aorta, however, it is sure that presence of viral genome does not always imply its pathogenecity. As important evidences indicating the pathogenesity of HCMV in human vascular diseases, our recent studies demonstrated virus-specific gene transcription in the surgical specimens of “inflammatory” aortic aneurysms but not in any other human aortic tissue, and also exhibited that the immediate early (IE) gene product stimulates vascular smooth muscle proliferation in rabbit carotid arteries. Whereas these findings suggest the pathogenic ability of HCMV in human vascular tree, some issues to be sollved including the reason why HCMV-IE does not induce any inflammatory response in rabbit. Clearly the immune system is varied among the species and this point should be studied very carefully. Overall, although it seems nearly slow, we consider that the studies for virus etiology in human vascular disorders are now getting progression.

著者

八木 俊一 市川 秀一 酒巻 哲夫 高山 嘉朗 村田 和彦 菅井 芳郎

出版者

一般社団法人 日本動脈硬化学会

雑誌

動脈硬化 (ISSN:03862682)

巻号頁・発行日

vol.15, no.1, pp.205-208, 1987-04-01 (Released:2011-09-21)

参考文献数

5

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We usually measure blood pressure of hemiparetic stroke patients with the intact upper arm. It is unclear whether the values of blood pressure of the paretic arms are higher or lower than those of the intact contralateral arms. Simultaneous measurements of blood pressure of bilateral arms of stroke patients were carried out using two automatic manometers in the present study.Subjects were forty-seven stroke patients in chronic phase. Thirty patients were diagnosed as cerebral hemorrhage and seventeen were the patients with cerebral infarction. Twenty patients had right hemiparesis and twenty-seven patients were with left hemiparesis. The patients were supine position for fifteen minutes before measurement of blood pressure. Blood pressure of bilateral arms was determined by inflating simultaneously cuffs after these of the two automatic manometers (TAKEDA MEDICAL, UA-254) attached to the both sides. Measurements were performed three times successively every minute, the manometers were changed with each other and three more determinations were carried out. The mean values for these six measurements were compared in each side of the paretic and intact arms. For the comparison of the thickness of each arm, we measured the circumference of bilateral arms at the 5cm proximal point from the elbow joint. P value of <0.05 was considered significant.Blood pressure of the paretic arm was 131±3mmHg (mean ± SE) in systolic and 83±1mmHg in diastolic. Blood pressure of the intact arm was 129±3mmHg in systolic and 78±1mmHg in diastolic. Both systolic and diastolic blood pressure of the paretic arm were significantly (p<0.01 and p<0.001 respectively) higher than those of the intact arm. The circumference of the paretic arm did not differ from that of the intact arm (21.6<0.3cm versus 21.9<0.3 cm).Because we have often observed muscle atrophy or edema in paretic extremities of stroke patients, the difference of blood pressure could be expected between the paretic and intact arm. In this study, both systolic and diastolic blood pressure of the paretic arm were higher than those of the intact arm. The difference of blood pressure does not seem to arise from unfitness of width of manometer's cuff for arm thickness since the thickness of paretic arm did not differ from that of intact arm.

著者

齊藤 昇 依岡 秀典 江渕 喜徳 土居 義典 小沢 利男 森木 利昭 原 弘

出版者

一般社団法人 日本動脈硬化学会

雑誌

動脈硬化 (ISSN:03862682)

巻号頁・発行日

vol.15, no.4, pp.997-1003, 1987-08-01 (Released:2011-09-21)

参考文献数

13

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One female inpatient, K. N., aged 57 years, suffered from liver cirrhosis, hypothyroidism, hypoadrenalism and bleeding tendency. After admission she received the administrations of diuretics such as furosemide and spironolactone to improve her anasarca, and also those of levothyroxine and prednisolone to fill up the deficient thyroid or adrenal functions.Slight to moderate elevations of transaminase activities, alkaline phosphatase activity of total bilirubin and increased gamma globulin concentrations were observed in this case, while low levels of triiodothyronine, total thyroxine, cortisol or urinary 17-hydroxycorticoid.Serum LCAT activity was extremely decreased, that was, 16nmol/ml/hr. She also had extremely low levels of serum lipids or apoproteins. For examples serum total cholesterol was 16mg/dl, triglyceride 18mg/dl, HDL-cholesterol 8mg/dl, phospholipid 27mg/dl, apoproteins A-I, C-II or C-III 0mg/dl, A-II 2.8mg/dl, B 10mg/dl and E 0.8mg/dl. VLDL-Chol, VLDL-TG, VLDL-PL and LDL-TG were smaller in the proportions of lipoprotein fractions, while HDL-TG, LDL-Chol and LDL-PL larger.One rare cirrhotic case with extremely low levels of serum lipids, apoproteins or lipoprotein fractions were shown in this article.

著者

中島 久実子

出版者

一般社団法人 日本動脈硬化学会

雑誌

動脈硬化 (ISSN:03862682)

巻号頁・発行日

vol.17, no.4, pp.553-570, 1989-08-01 (Released:2011-09-21)

参考文献数

69

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The interstitial fluid of arterial tissue constitutes a milieu interior for the intimal smooth muscle cells. Atherosclerotic foam cells arise from these cells when they are exposed to a high concentration of interstitial LDL. However, the available data on the concentrations of interstitial lipoproteins including LDL in normal arteries is unequivocal.In this study, the author has demonstrated the localization, form, and concentration of interstitial lipoproteins in terms of their apoproteins of A-I, A-II, B, C-II, C-III, and E in the normal intimas from 26 thoracic aortas and 19 pulmonary arteries of 45 autopsied cases (male 23, female 22, age 54±19).The localization of apoproteins was observed in the normal Intima of thoracic aorta and pulmonary artery by immunofluorescence: apo B localized mainly around the cell in the intima and along the internal elastic lamina, while apo A-I and A-II grouped in an islet form deep in the media. Apo C-II, C-III and E were sparsely present both in the intima and media. SDS-gradient PAG electrophoresis revealed all serum apoprotein bands with a _mobility corresponding to the serum counterparts.PAG disk electrophoresis showed that apoproteins were complexed with sudanophillic lipids in the interstitial fluid, and migrated in 3 bands of mobility corresponding to those of serum VLDL, LDL and HDL. Three dimensional form of recovered lipid-apoprotein complex was confirmed to be spheres of a diameter of about 500Å and 200Å by transmission and scanning electronmicroscopy.The concentrations of interstitial lipoproteins were measured by SRID for both thoracic and pulmonary interstitial fluids. Apo A-I was 5.2mg/ dl in thoracic aorta, while 0.4mg/dl in pulmonary artery, A-II 1.4mg/dl vs. 0.9mg/dl, B 18.2mg/dl vs. 10.3mg/dl, C-II 0.2mg/dl vs. 0.2mg/dl, C-III 1.1mg/dl vs. 0.4mg/dl and E 2.1mg/dl vs. 0.6mg/ dl, respectively. The concentrations of apoproteins gained in wet tissue weight were converted to the unit of mg/dl by using factors for water content of thoracic aorta 77±10%, and for pulmonary artery 81±19%, and the volume of extracellular space as 52±10% and 68±11%, respectively.These indicated that there exists a distinct difference in the concentrations of lipoproteins between serum and interstitial fluid of both thoracic and pulmonary intimas: interstitial LDL is 1/5 to 1/10 of serum counterpart, VLDL 1/5 to 1/7, and HDL 1/25 to 1/35, respectively. These lipoprotein levels maintained as a millieu interior by an endothelial barrier provides intimal cells with cholesterol necessary but sufficient for their cellular metabolism. The alteration in this milieu interior brings forth the accumulation of cholesterol in the cytoplasma of intimal cells, and leads to the formation of foam cells in the intima.

著者

一瀬 白帝

出版者

一般社団法人 日本動脈硬化学会

雑誌

動脈硬化 (ISSN:03862682)

巻号頁・発行日

vol.23, no.9, pp.533-538, 1996-04-10 (Released:2011-09-21)

参考文献数

18

著者

武永 美津子 平井 愛山 寺野 隆 田村 泰 北川 晴雄 吉田 尚

出版者

一般社団法人 日本動脈硬化学会

雑誌

動脈硬化 (ISSN:03862682)

巻号頁・発行日

vol.13, no.4, pp.825-829, 1985-10-01 (Released:2011-09-21)

参考文献数

15

被引用文献数

1

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Arachidonic acid (AA) is well known to be metabolized to thromboxane (TX) A2, 12-hydroxyheptadecatrienoic acid (HHT) and 12-hydroxyeicosatetraenoic acid (12-HETE) in platelets. Prostaglandin endoperoxides and TXA2 are known to be potent aggregating agents. On the otherr hand, the labile 12-lipoxygenase metabolite, 12-hydroperoxyeicosatetraenoic acid (12-HPETE) has been reported to have a rather anti-aggregating action.It has been reported that eicosapentaenoic acid (EPA) has a potent inhibitory effect on platelet aggregation. TXA3 produced from EPA is nonaggregating agent. Although major metabolites of EPA in platelets is said to be those of 12-lipoxygenase pathway, the effect of them has not been clearly elucidated yet. So, we examined the effect of 12-lipoxygenase metabolites of EPA on platelet function and compared them with those of AA.Both of the labile 12-lipoxygenase metabolites, 12-HPETE and 12-hydroperoxyeicosapentaenoic acid (12-HPEPE) suppressed dose-dependently platelet aggregation and release of 5-hydroxytryptamine (5-HT) induced by collagen and AA, while 12-HETE and 12-hydroxyeicosapentaenoic acid (12-HEPE) had no such effects. The inhibitory effects of these 12-hydroperoxy compounds on platelet aggregation and release reaction seem to be almost equipotent. However, 5- and 15-hydroperoxy isomers were less potent in inhibiting aggregation.These results may indicate that 12-HPETE and 12-HPEPE have a potent anti-aggregatory activity and may play a role in regulating platelet aggregability.

著者

高沢 謙二 黒須 富士夫 斎木 徳祐 安藤 寿章 奥秋 勝彦 Ranjeet S BARAL 田中 信大 伊吹山 千晴

出版者

一般社団法人 日本動脈硬化学会

雑誌

動脈硬化 (ISSN:03862682)

巻号頁・発行日

vol.26, no.11-12, pp.313-319, 1999-10-20 (Released:2011-09-21)

参考文献数

25

被引用文献数

1 2

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Second derivative of photoplethysmogram (SDPTG) or acceleration plethysmogram (APG) is the second derivative wave of the photoplethysmogram. SDPTG has been developed to allow more accurate recognition of the inflection points on the original plethysmographic wave, ie, anacrotic or dicrotic notches. It has been recognized as the independent examination tool for vascular properties.SDPTG includes 4 systolic waves and 1 diastolic wave, namely a-wave (early systolic positive wave), b-wave (early systolic negative wave), c-wave (late systolic reincreasing wave), d-wave (late systolic redecreasing wave) and e-wave (early diastolic positive wave).The ratio of the height of the each wave to that of the a-wave (b/a, c/a, d/a and e/a) are usually used for wave analyses.We previously reported characteristic age related wave changes of SDPTG in 600 subjects (each 50 men and 50 women in each decade from the 3rd to the 8th) in our health assessment center (Hypertension, 1998; 32: 365-370.).SDPTG-AI increased with augmentation index of original photoplethysmogram which is known to increase with increasing age, and is defined as the ratio of the amplitude of the late systolic component to that of the early systolic component of the original plethysmogram.The b/a increased with age and c/a, d/a and e/a decreased with age. Thus b-c-d-e/a was taken as the aging index of SDPTG (SDPTG aging index: SDPTGAI) which increased with age. SDPTG (Y) increased with age (X) as Y=0.023X-1.515, r=0.80, p<0.001).Similar distribution of vascular ages were seen in different districts group, including women in Toba city in Mie prefecture, subjects in shinjuku in Tokyo and patients in Broussias Hospital in Paris.SDPTGAI increased with vascular stiffness represented by inceased b/a and increasing reflection wave represented by decreasing d/a. Vascular age estimated by SDPTG=43.5SDPTGAI+65.9.SDPTGAI and vascular age were significantly higher in patients with a history of hypertension, diabetes mellitus, and hyperlipidemia than in subjects without any disease (p<0.01).The possibility of arteriosclerotic disease can be considered when vascular age is higher than actual age. Though cut-off value of the difference of vascular and actual ages are under examination, the tentative cut-off value is a difference of 15-20 years, which will provide us useful information for screening of arteriosclerotic diseases.A new type of apparatus (Fukuda FCP series) provides full automated detection of each indices and prints out within 30 seconds, which will be widely used for noninvasive evaluation of arteriosclerosis through vascular age.

著者

下村 伊一郎

出版者

一般社団法人 日本動脈硬化学会

雑誌

動脈硬化 (ISSN:03862682)

巻号頁・発行日

vol.23, no.7-8, pp.443-449, 1996-03-30 (Released:2011-09-21)

参考文献数

7

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We clarified, using computed tomography, that the accumulation of intra-abdominal visceral fat is more closely associated with complications of obesity, such as diabetes mellitus, hyperlipidemia, hypertension and ischemic heart disease, than the accumulation of subcutaneous fat. Recently we also revealed that the accumulation of intra-abdominal visceral fat correlates to metabolic disorders and cardiovascular disease even in normal weight subjects. From these backgrounds, we proposed the concept of “visceral fat syndrome”, a multiple risk factors-clustering syndrome for the incidence of atherosclerosis. Clinically, it was showed that the reduction of visceral fat should be important for the cure and the protection of these disorders. We have suggested that physical exercise reduced accumulated visceral fat prominently from the observations of the effect of exercise therapy on visceral fat type obesity and the analysis of fat distribution of sumo wrestlers. In order to clarify the effect of exercise on fat distribution, the mRNA levels and the activities of acyl-CoA synthetase (ACS), lipoprotein lipase (LPL), and glucose transporter (Glut 4) in some tissues were compared between exercised and sedentary rats. We observed rapid decrease of tissue weight in visceral fat through marked decrease of ACS, LPL, and Glut 4 parameters, while there were no alterations of them in subcutaneous fat. Gastrocnemius muscle was heavier in exercised rats, and ACS activity elevated in the gastrocnemius muscle of the exercised rats. By analyzing the expressed genes in human visceral fat, compared with human subcutaneous fat and various tissues, visceral fat was showed to have a higher metabolic activity at fene expression level. We suggest that intra-abdominal visceral fat may contribute to switching of distribution of plasma energy flux, including lipid and glucose, from fat tissue to muscle in physical exercise, in rapid response to exercise from transcriptional level.

著者

原納 優

出版者

一般社団法人 日本動脈硬化学会

雑誌

動脈硬化 (ISSN:03862682)

巻号頁・発行日

vol.27, no.12, pp.313-318, 2000-06-20 (Released:2011-09-21)

参考文献数

15

被引用文献数

1

著者

岩崎 勤 松下 哲 折茂 肇 白木 正孝 萬木 信人 加藤 洋一 高橋 龍太郎 蔵本 築 村上 元孝 野間 昭夫 岡部 紘明

出版者

一般社団法人 日本動脈硬化学会

雑誌

動脈硬化 (ISSN:03862682)

巻号頁・発行日

vol.9, no.1, pp.87-91, 1981-04-01 (Released:2011-09-21)

参考文献数

13

被引用文献数

1

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Effects of γ-oryzanol on the serum HDL, LDL and total cholesterol (ch) and Triglyceride were studied in 17 cases whose age was 47 years old to 82 years old (mean age was 71.2 years old). Three hundred mg/day of γ-oryzanol was given for 4 months.Serum HDL-ch was 50.0±2.3 (m±SE) mg/dl before γ-oryzanol and 53.7mg/dl 3 months later but HDL-ch which was below 50mg/dl before treatment increased significantly (p<0.01) from 43.3mg/dl to 50.4mg/dl 2 months later. HDL-ch below 45mg/dl increased significantly (p<0.01) from 41.3mg/dl (mean) to 50.0mg/dl (mean) 2 months later. LDL-ch decreased significantly (p<0.01) from 158.8mg/dl to 134.8mg/dl after γ-oryzanol. HDL-ch×10/LDL-ch showed significant changes (p<0.01) (from 3.26 to 4.14) 2 months later. Total-ch did not change and between 203mg/dl and 208mg/dl. Triglyceride did not show significant changes.It is suggested that γ-oryzanol alters the metabolism of HDL-ch and LDL-ch and increases serum low HDL-ch.

出版者

一般社団法人 日本動脈硬化学会

雑誌

動脈硬化 (ISSN:03862682)

巻号頁・発行日

vol.27, no.Supplement1, pp.101-135, 1999-10-25 (Released:2011-09-21)

著者

内藤 周幸 川村 光信 橋本 佳明

出版者

一般社団法人 日本動脈硬化学会

雑誌

動脈硬化 (ISSN:03862682)

巻号頁・発行日

vol.11, no.2, pp.239-250, 1983-06-01 (Released:2011-09-21)

参考文献数

15

被引用文献数

5

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We have studied the effects of polyunsaturated fatty acids on platelet aggregation, mainly by means of the in vitro experiments. The results of the studies are as follows.1) It seemed that arachidonic acid aggregated platelets as well irrespective of prostaglandin pathways.2) Eicosapentaenoic acid (EPA) inhibited platelet aggregation caused by various aggregating agents, such as ADP, collagen, and arachidonic acid, when platelet-rich plasma was used. However, EPA itself aggregated platelets when washed platelets were used.3) On the other hand, such a low concentration of EPA as not to induce platelet aggregation by itself, inhibited platelet aggregation caused by arachidonic acid, even when washed platelets were used.4) From these results it was thought that the inhibitory effect of EPA on platelet aggregation was not active and direct one but indirect due to reducing the use of arachidonic acid in the platelets.5) It was suggested that the ratio of EPA to arachidonic acid in phospholipid fatty acids of platelets needed to be more than 0.14, in order that EPA had an inhibitory effect on platelet aggregation.6) It is considered that the optimum balance between ω-3 species of fatty acids and ω-6 ones in phosphlipid fatty acids of platelets is necessary for platelets to function normally.

著者

寺本 民生 木下 誠 桂川 敬太 岡崎 聡子 山中 正己 永好 昭 大濱 知子 松木 則夫 斎藤 洋

出版者

一般社団法人 日本動脈硬化学会

雑誌

動脈硬化 (ISSN:03862682)

巻号頁・発行日

vol.22, no.6-7, pp.465-470, 1994-10-25 (Released:2011-09-21)

参考文献数

6

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We found that fatty liver is easily induced in a novel experimental animal, Suncus murinus (suncus) by withholding food. Hepatic triglyceride content increased linearly for up to 24 hours after fasting in these animals, while the glycogen content decreased. Although the glycogen contents returned to the level before fasting at 12 hours after refeeding, the triglyceride contents decreased gradually but did not reach to the level before fasting even at 24 hours after refeeding in suncus. Plasma lipids, glucose and insulin levels decreased by fasting and returned to the levels before fasting between 8 and 24 hours after refeeding. On the other hand, the plasma levels of free fatty acid and ketone bodies were elevated significantly by fasting and decreased rapidly by refeeding. These responses to fasting and refeeding except for the change in hepatic triglyceride are in common with other experimental animals, suggesting that there are no abnormalities not only in glucose metabolism but also in fatty acid metabolism.The study of lipoproteins from this animal revealed that small amounts of lipoproteins with apolipoprotein (apo) E but without apoB were observed in the fraction of density less than 1.08g/ml. In order to learn whether apoB is synthesized by the liver or not, isolated suncus livers were perfused with an addition of [35S] methionine. Small amounts of radioactivity were observed in apoE of VLDL, and fairly large amounts in apoE and A-I in the fraction of LDL+HDL, suggesting that VLDL was secreted with apoE but not with apoB from the liver. Northern blot analysis with use of rat apoB cDNA revealed a weak signal of hybridized rat apoB cDNA between 15Kb and 9Kb in the suncus liver and intestinal mucosa; this is almost the same size as rat apoB mRNA. This finding suggests the presence of apoB mRNA in the suncus.In conclusion, apoB is not secreted from the suncus liver, owing to a defect in intracellular posttranscriptional processing or to ineffective transcription. This might be one of the reasons for fatty deposits in the suncus liver. Suncus may be a candidate for an animal model of abetalipoproteinemia as well as fatty liver due to a defect of apoB synthesis.

著者

秦 葭哉 及川 眞一 山田 信博 山下 静也 佐久間 長彦 佐々木 淳 横山 信次 馬 渕宏 齋藤 康 板倉 弘重 江草 玄士 井藤 英喜 寺本 民夫 都島 基夫 多田 紀夫

出版者

一般社団法人 日本動脈硬化学会

雑誌

動脈硬化 (ISSN:03862682)

巻号頁・発行日

vol.26, no.9, pp.217-218, 1999

著者

後藤 由夫 阿部 隆三 及川 真一 佐野 隆一 藤井 豊

出版者

Japan Atherosclerosis Society

雑誌

動脈硬化 (ISSN:03862682)

巻号頁・発行日

vol.12, no.6, pp.1351-1357, 1985

著者

阿部 隆三 及川 真一 佐野 隆一 藤井 豊 義江 和子 後藤 由夫

出版者

Japan Atherosclerosis Society

雑誌

動脈硬化 (ISSN:03862682)

巻号頁・発行日

vol.12, no.4, pp.951-956, 1984

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In the present study, effect of probucol (500mg×2, daily) on serum lipids and lipoproteins in S. F. family who showed typical familial hyper-cholesterolemia (FH) were investigated. The results were as follows:<br>1) The values of serum cholesterol (TC) before and after treatment of probucol were 348±14mg/dl and 220±12mg/dl, respectively. These data shows significant reduction of serum cholesterol after treatment of probucol. The percent of reduction of serum cholesterol was 36.4%. This value was significantly higher than the value (17%) in the other FH.<br>2) Though levels of LDL- and HDL-C were decreased after treatment of probucol, LDL-C level was markedly decrease in S. F family as compared with other FH. Reduction of HDL-C after treatment of probucol was not significant difference between in S. F family and other FH.<br>3) Total biliary lipids after treatment of probucol increased from 68.8μmol/ml to 141.9μmol/ml in a patient of S. F family. Level of bile acid increased after treatment of probucol, especially, in this case. On the other hand, the change of biliary lipids after treatment of probucol was not seen in other FH.<br>From these data, though mechanism of marked reduction of LDL-C in S. F family after treatment of probucol was still unclear, metabolism of cholesterol to synthesis of bile acid might have especially involved to marked reduction of serum LDL-C in S. F family.

著者

小竹 英俊 及川 眞一 生井 一之 佐久間 恵理子 義江 和子 豊田 隆謙

出版者

Japan Atherosclerosis Society

雑誌

動脈硬化 (ISSN:03862682)

巻号頁・発行日

vol.17, no.3, pp.453-456, 1989

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It has been established previously that low density lipoprotein (LDL) incubated with cultured endothelial cells, smooth muscle cells, or macrophages undergo a free radical-catalyzed oxidative modification. This modification involves lipid peroxides and extensive structural changes in the LDL molecule. The oxidatively modified LDL inhibits the motility of the mouse resident peritoneal macrophages, yet lysophosphatidylcholine in oxidized LDL acts as a chemotactic factor for circulating human monocytes. The present study demonstrated that the stimulation of human mononuclear cells with phorbol myristate acetate (PMA) results in an increase of oxidized LDL, which was assayed by LPO-KIT (obtained from Kyowa Hakko Inc.) in the culture medium. The medium containing oxidized LDL did not exhibit a chemotactic for human monocytes, but inhibited the chemotactic responses of monocytes to the N-formyl peptide and aortic extract.<br>These findings suggest that circulating monocytes may play a role in the subendothelial space by some chemotactic factors and become trapped in the splace due to the inhibitory effects of oxidatively modified LDL.

著者

佐野 隆一 藤井 豊 義江 和子 及川 真一 阿部 隆三 豊田 隆謙 後藤 由夫

出版者

一般社団法人 日本動脈硬化学会

雑誌

動脈硬化 (ISSN:03862682)

巻号頁・発行日

vol.13, no.2, pp.349-353, 1985

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Three dysbetalipoproteinemic subjects with the E 2/2 homozygous phenotype were analysed serum lipoproteins and serum apolipoprotein E and B levels. Two subjects were normocholesterolemic and one was hypercholesterolemic (type III hyperlipoproteinemia). A patient with type III phenotype had cholesterol-rich VLDL, although the other two normocholesterolemic subjects didn't have it. Both normo- and hypercholesterolemic subjects had elevated levels of serum apo E and a reduction in serum apo B. Therefore, serum apo E/apo B ratio of a patient with type III phenotype was extremely high as compared with other lipoprotein disorders. These ratio of normocholesterolemic dysbetalipoproteinemia was also high as compared with those except for type V hyperlipoproteinemia. It is suggested that dysbetalipoproteinemia might be easily caught in general population by screening examination of serum apo E/apo B ratio.

著者

及川 眞一 堀 三郎

出版者

Japan Atherosclerosis Society

雑誌

動脈硬化 (ISSN:03862682)

巻号頁・発行日

vol.14, no.1, pp.25-29, 1986

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It would be important to study the characteristics and the action of "dentured" or "modified" LDL in atherogenesity. We investigated whether modified LDL was formed in normal human and what effect this LDL had on human arterial smooth muscle cells in culture.<br>Incubation of plasma prepared from normal human was performed at 37°C for 6 hours. Thrombin was added to both of incubated plasma and also non-incubated plasma obtained from each subject in order to remove fibrin. Lipoproteins (VLDL, IDL, LDL and HDL) were ultracentrifugally isolated from each serum.<br>LDL obtained from incubated plasma had faster mobility than that from non-incubated plasma on agarose-gel electrophoresis. Lipids-composition (TC, TG, PL and Ch-ester) was altered in LDL after incubation. These alterations of LDL might be induced by lipid transfer protein. In this way, LDL obtained from normal human was modified.<br>DNA synthesis of human arterial smooth muscle cells (SMC) increased in the culture with LDL addition. This action of LDL was dose-dependent manner. DNA synthesis increased in the culture with modified LDL more than that with native LDL.<br>These data indicated that LDL obtained from normal human was easily modified and modified LDL influenced DNA synthesis of cultured arterial SMC.