著者
中島 久実子
出版者
一般社団法人 日本動脈硬化学会
雑誌
動脈硬化 (ISSN:03862682)
巻号頁・発行日
vol.17, no.4, pp.553-570, 1989-08-01 (Released:2011-09-21)
参考文献数
69

The interstitial fluid of arterial tissue constitutes a milieu interior for the intimal smooth muscle cells. Atherosclerotic foam cells arise from these cells when they are exposed to a high concentration of interstitial LDL. However, the available data on the concentrations of interstitial lipoproteins including LDL in normal arteries is unequivocal.In this study, the author has demonstrated the localization, form, and concentration of interstitial lipoproteins in terms of their apoproteins of A-I, A-II, B, C-II, C-III, and E in the normal intimas from 26 thoracic aortas and 19 pulmonary arteries of 45 autopsied cases (male 23, female 22, age 54±19).The localization of apoproteins was observed in the normal Intima of thoracic aorta and pulmonary artery by immunofluorescence: apo B localized mainly around the cell in the intima and along the internal elastic lamina, while apo A-I and A-II grouped in an islet form deep in the media. Apo C-II, C-III and E were sparsely present both in the intima and media. SDS-gradient PAG electrophoresis revealed all serum apoprotein bands with a _mobility corresponding to the serum counterparts.PAG disk electrophoresis showed that apoproteins were complexed with sudanophillic lipids in the interstitial fluid, and migrated in 3 bands of mobility corresponding to those of serum VLDL, LDL and HDL. Three dimensional form of recovered lipid-apoprotein complex was confirmed to be spheres of a diameter of about 500Å and 200Å by transmission and scanning electronmicroscopy.The concentrations of interstitial lipoproteins were measured by SRID for both thoracic and pulmonary interstitial fluids. Apo A-I was 5.2mg/ dl in thoracic aorta, while 0.4mg/dl in pulmonary artery, A-II 1.4mg/dl vs. 0.9mg/dl, B 18.2mg/dl vs. 10.3mg/dl, C-II 0.2mg/dl vs. 0.2mg/dl, C-III 1.1mg/dl vs. 0.4mg/dl and E 2.1mg/dl vs. 0.6mg/ dl, respectively. The concentrations of apoproteins gained in wet tissue weight were converted to the unit of mg/dl by using factors for water content of thoracic aorta 77±10%, and for pulmonary artery 81±19%, and the volume of extracellular space as 52±10% and 68±11%, respectively.These indicated that there exists a distinct difference in the concentrations of lipoproteins between serum and interstitial fluid of both thoracic and pulmonary intimas: interstitial LDL is 1/5 to 1/10 of serum counterpart, VLDL 1/5 to 1/7, and HDL 1/25 to 1/35, respectively. These lipoprotein levels maintained as a millieu interior by an endothelial barrier provides intimal cells with cholesterol necessary but sufficient for their cellular metabolism. The alteration in this milieu interior brings forth the accumulation of cholesterol in the cytoplasma of intimal cells, and leads to the formation of foam cells in the intima.
著者
張 念中 河合 祥雄 岡田 了三
出版者
一般社団法人 日本動脈硬化学会
雑誌
動脈硬化 (ISSN:03862682)
巻号頁・発行日
vol.15, no.5, pp.1185-1191, 1987-10-01 (Released:2011-09-21)
参考文献数
25

Mental stress from one's occupational circumstances is considered an important risk factor in the occurrence of myocardial infarction. However, because of difficulty in quantitative evaluation of such stress, this issue has not been fully studied. In the present work, we investigated the relationship between deaths from acute myocardial infarction (AMI) and occupation using data from the series of the Annual of the Pathological Autopsy Cases in Japan, in which autopsy cases throughout Japan are described. With regard to the 56, 268 persons who died at the age of 15 or older in 1973, 1974 and 1975, i. e., the year of economic depression due to the first oil shock and the years before and after the oil shock, we compared (I) the mortalities from AMI and frequencies of such deaths among all autopsy cases in Japan in relation to the occupational group in the three years and (II) the mortalities from AMI according to the occupation among 3, 241 randomly selected deaths in the three years. The mortality from AMI for 1973, 1974 and 1975 was 2.6%, 3.7% and 3.0%, respectively, showing a significantly higher value for 1974 (p<0.001). Among persons who died from AMI, the proportion of mental workers, particularly executives and managers, and unemployed/retired individuals was significantly higher (p<0.05) in 1974. As for randomly selected autopsy cases, the mortalities from AMI among mental workers in 1973, 1974 and 1975 were 7.8%, 17.0% and 6.2%, respectively, and the corresponding rates among physical workers were 2.0 %, 1.8% and 2.6%. Thus, the mortality among mental workers was significantly increased in 1974 (p<0.05), whereas there were no such variations among physical workers. In addition to the corroboration with the previous experience that deaths from AMI are more frequent among mental workers, particularly executives and managers, than among physical workers, we noted an increase in moratlity from AMI in the period of economic depression. It seems that stress derived from occupational circumstances rather than limited physical activities is involved in the occurrence of AMI.
著者
吉川 昌江 佐久間 長彦 日比 野剛 池内 玲子 佐藤 貴昭 米山 明彦 岩田 誠司 川口 正展 神谷 吉宣 伊藤 純子 藤浪 隆夫
出版者
一般社団法人 日本動脈硬化学会
雑誌
動脈硬化 (ISSN:03862682)
巻号頁・発行日
vol.22, no.2-3, pp.203-207, 1994-08-01 (Released:2011-09-21)
参考文献数
25
被引用文献数
1 1

The Oxidative modification of low-density lipoprotein (LDL) could contribute to atherosclerosis as a result of its cytotoxic effect, uptake by the scavenger receptor, and its influence on monocyte and macrophage migration. Ascorbate is an important watersoluble, chain-breaking antioxidant in humans.In this study, we examined the effect of ascorbate on the Cu2+-induced oxidative modification of LDL. LDL was incubated for 24 hours with 2.5μM copper (Cu2+) in phosphate-buffered saline (PBS) in both the presence and absence of ascorbic acid (20μg/ml, 25μg/ml, 30μg/ml, and 35μg/ml). Ascorbate significantly inhibited the oxidative modification of LDL, as indicated by both the decreased electrophoretic mobility and the linoleic acid content. Oxidative modification was prevented in a concentration-dependent manner by the addition of ascorbate.Our data suggests that ascorbate may play an important physiological role in protection against the oxidative modification of LDL.
著者
八木 俊一 市川 秀一 酒巻 哲夫 高山 嘉朗 村田 和彦 菅井 芳郎
出版者
一般社団法人 日本動脈硬化学会
雑誌
動脈硬化 (ISSN:03862682)
巻号頁・発行日
vol.15, no.1, pp.205-208, 1987-04-01 (Released:2011-09-21)
参考文献数
5

We usually measure blood pressure of hemiparetic stroke patients with the intact upper arm. It is unclear whether the values of blood pressure of the paretic arms are higher or lower than those of the intact contralateral arms. Simultaneous measurements of blood pressure of bilateral arms of stroke patients were carried out using two automatic manometers in the present study.Subjects were forty-seven stroke patients in chronic phase. Thirty patients were diagnosed as cerebral hemorrhage and seventeen were the patients with cerebral infarction. Twenty patients had right hemiparesis and twenty-seven patients were with left hemiparesis. The patients were supine position for fifteen minutes before measurement of blood pressure. Blood pressure of bilateral arms was determined by inflating simultaneously cuffs after these of the two automatic manometers (TAKEDA MEDICAL, UA-254) attached to the both sides. Measurements were performed three times successively every minute, the manometers were changed with each other and three more determinations were carried out. The mean values for these six measurements were compared in each side of the paretic and intact arms. For the comparison of the thickness of each arm, we measured the circumference of bilateral arms at the 5cm proximal point from the elbow joint. P value of <0.05 was considered significant.Blood pressure of the paretic arm was 131±3mmHg (mean ± SE) in systolic and 83±1mmHg in diastolic. Blood pressure of the intact arm was 129±3mmHg in systolic and 78±1mmHg in diastolic. Both systolic and diastolic blood pressure of the paretic arm were significantly (p<0.01 and p<0.001 respectively) higher than those of the intact arm. The circumference of the paretic arm did not differ from that of the intact arm (21.6<0.3cm versus 21.9<0.3 cm).Because we have often observed muscle atrophy or edema in paretic extremities of stroke patients, the difference of blood pressure could be expected between the paretic and intact arm. In this study, both systolic and diastolic blood pressure of the paretic arm were higher than those of the intact arm. The difference of blood pressure does not seem to arise from unfitness of width of manometer's cuff for arm thickness since the thickness of paretic arm did not differ from that of intact arm.
著者
佐藤 敬 高松 滋 逸見 一穂 作田 茂 水野 成徳 目時 弘文 高松 むつ
出版者
一般社団法人 日本動脈硬化学会
雑誌
動脈硬化 (ISSN:03862682)
巻号頁・発行日
vol.7, no.2, pp.271-277, 1979-07-01 (Released:2011-09-21)
参考文献数
12

The HDL-cholesterol (HDL-Ch) concentration of 153 cerebrocascular patients within 1 year from onset and 257 controls were determined in the supernatant of serum in heparin-Ca++ precipitation method. In some patients, serum HDL-phospholipid (HDL-PL) concentrations were also determined. Liver function tests (GOT, GPT, TTT, ZST) were normal in all of these subjects, and control subjects had no adnormalities in physical findings, blood pressure, ECG, urinalysis etc.The average values in male and female controls were 65±20.9mg/dl and 65±22.3mg/dl, respectively. The value of female subjects over 55 years of age was lower than those of age-matched male and female under 54. There was no significant change by age in values of male control. In male patients with cerebral infarction, average HDL-Ch value was 55±18.6mg/dl and it was significantly lower than the control value. The value of female patients was 62±15.0mg/dl. In female, there was no significant difference between patients and control. Among the patients with cerebral infarction, the cases with angiographically demonstrated obstruction of internal carotid or middle cerebral arteries showed lower value as compared to those without such findings. In male and female patients with cerebral hemorrhage, average HDL-Ch concentrations were 55±13.3mg/dl and 60±16.8mg/dl respectively. Again only the value of male patients was significantly lower than that of control.Considering the importance of balance between atherogenic and anti-atherogenic properties of cholesterol in various lipoprotein fractions, HDL-cholesterol/total cholesterol (HDL-Ch/TC) ratio was calculated in each subjects. The average values of this ratio in healthy men and women were 0.37±0.13 and 0.37±0.14, respectively. The value of male patients with cerebral infarction was 0.30±0.10, it was significantly lower than the control value. The values of female patients with cerebral infarction, male and female patients with cerebral hemorrhage were 0.33±0.09, 0.35±0.10 and 0.35±0.11, respectively. These values exhibited no significant difference when compared with each controls. From these results, it was recognized that at a given concentration of serum total cholesterol, male patients with cerebral infarction had relatively less cholesterol in their HDL fraction as compared to healthy subjects.Serum HDL-PL concentrations in male patients either with cerebral infarction or cerebral hemorrhage, were significantly lower than the control value. The difference between female patients and control was insignificant.These results may suggest the importance of HDL-Ch for the development of cerebral atherosclerosis and subsequent cerebral infarction at least in man.
著者
森 聖二郎
出版者
一般社団法人 日本動脈硬化学会
雑誌
動脈硬化 (ISSN:03862682)
巻号頁・発行日
vol.26, no.2, pp.81-86, 1998-10-10 (Released:2011-09-21)
参考文献数
19

Glucocorticoid-induced alterations of blood lipoprotein metabolism were analyzed in patients with various collagen diseases. Serum triglyceride concentration was rapidly and transiently increased by prednisolone treatment; it reached the maximal level after 2 weeks, and then gradually decreased to the initial level. On the other hand, serum total cholesterol concentration was slowly increased to the maximal level by 8 weeks and, thereafter, remained constant. Mathematical analysis has revealed that the average prednisolone-induced increase was about 2.5mg/dl/mg prednisolone for both serum total cholesterol and triglyceride levels. Ultracentrifugation analysis showed that not only low density but also very low density and high density lipoproteins were increased by prednisolone treatment. In a female patient with angina pectoris and xanthelasma, probucol treatment effectively normalyzed her glucocorticoid-induced hypercholesterolemia and, furthermore, the effective lowering of serum cholesterol level was also followed by successful cessation of angina attack and healing of xanthelasma. The data suggest the possible importance of glucocorticoid-induced hyperlipidemia in the development of atherosclerosis.
著者
張 念中 河合 祥雄 岡田 了三
出版者
Japan Atherosclerosis Society
雑誌
動脈硬化 (ISSN:03862682)
巻号頁・発行日
vol.15, no.5, pp.1185-1191, 1987

Mental stress from one's occupational circumstances is considered an important risk factor in the occurrence of myocardial infarction. However, because of difficulty in quantitative evaluation of such stress, this issue has not been fully studied. In the present work, we investigated the relationship between deaths from acute myocardial infarction (AMI) and occupation using data from the series of the Annual of the Pathological Autopsy Cases in Japan, in which autopsy cases throughout Japan are described. With regard to the 56, 268 persons who died at the age of 15 or older in 1973, 1974 and 1975, i. e., the year of economic depression due to the first oil shock and the years before and after the oil shock, we compared (I) the mortalities from AMI and frequencies of such deaths among all autopsy cases in Japan in relation to the occupational group in the three years and (II) the mortalities from AMI according to the occupation among 3, 241 randomly selected deaths in the three years. The mortality from AMI for 1973, 1974 and 1975 was 2.6%, 3.7% and 3.0%, respectively, showing a significantly higher value for 1974 (p<0.001). Among persons who died from AMI, the proportion of mental workers, particularly executives and managers, and unemployed/retired individuals was significantly higher (p<0.05) in 1974. As for randomly selected autopsy cases, the mortalities from AMI among mental workers in 1973, 1974 and 1975 were 7.8%, 17.0% and 6.2%, respectively, and the corresponding rates among physical workers were 2.0 %, 1.8% and 2.6%. Thus, the mortality among mental workers was significantly increased in 1974 (p<0.05), whereas there were no such variations among physical workers. In addition to the corroboration with the previous experience that deaths from AMI are more frequent among mental workers, particularly executives and managers, than among physical workers, we noted an increase in moratlity from AMI in the period of economic depression. It seems that stress derived from occupational circumstances rather than limited physical activities is involved in the occurrence of AMI.