著者

斎藤 隆三 横山 定助

出版者

昭和大学学士会

雑誌

昭和医学会雑誌 (ISSN:00374342)

巻号頁・発行日

vol.17, no.2, pp.141-146, 1957-05-30 (Released:2010-09-09)

参考文献数

11

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In the present work, a few observations were made on the fixation of the tissues, particularly on the time between the collection and fixation of the tissues and its procedures, and the preparation of extra-thin sections with ultramicrotome, which are most essential for obtaining superior histological pictures in the electron microscopy.In the cases of the tissues obtained in the animal experiments, several drops of OsO4 solution were dripped on the tissue when the material was collected and the tissue shall be cut into oblong slices; slices shall be placed in OsO4 solution and cut into fine blocks of approximately 1 mm3 20-30 minutes later. The fine blocks of approximately 1 mm3 shall be obtained by 2-3 cuts with razor blade. On some occasions, autolytic changes are observed in the picture of the material prepared from the centre of the fine blocks fixed for 1-2 minutes. This is considered due to the time required for the OsO4 solution to penetrate the tissue. When mechanical force was added to this, the picture thus obtained might be misinterpreted. In the case of human tissue, surgical specimen is preferable, but, if too long time was spent for the operation, fine structures of the cells was lost, and conglomeration and sissolution of the granules contained in the nucleus were observed. These phenomena were particularly eminent in the cases of tumor cells. In the cases of autopsy material, unless the material was collected within 1 hour at the latest from the death, the significance of electron microscopy will be lost.