著者

市田 篤郎 小田島 武志 水野 守道 西風 脩

出版者

Japanese Association for Oral Biology

雑誌

歯科基礎医学会雑誌 (ISSN:03850137)

巻号頁・発行日

vol.21, no.2, pp.372-376, 1979 (Released:2010-10-28)

参考文献数

14

被引用文献数

1 1

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生後4週の雌マウスにビタミンAパルミテート1万単位を毎日投与して, 顎下腺におけるシアル酸含量, CMP-シアル酸合成酵素 (CSS) 及びシアル酸転移酵素 (ST) 活性の変動を追及した。ビタミンA過剰投与は糖タンパク合成を亢進させるという従来の報告に反してシアル酸含量, ST活性は投与日数の経過と共に低下した。これに対してCSS活性は初期に低下がみられたが7~9週目には対照群のレベルに回復した。9週目においてはNeuraminidase活性は対照群との間に差が認められなかった。シアル酸含量の低下はST活性の低下によることが示唆された。

著者

水野 守道

出版者

日本結合組織学会

雑誌

Connective tissue (ISSN:0916572X)

巻号頁・発行日

vol.32, no.1, pp.69-75, 2000-03-25

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The regeneration of teeth and bone becomes to be a new therapy in the near future. Our group have been proposed that the extracellular matrix is an powerful stimulator for the regeneration of tissues. Recently we found that bone marrow cells which were harvested from rat femur, differentiated into osteoblasts when cells were cultured with type I collagen. Type II, III, and V collagen did not show the inducing activity. Therefore the inducing activity of type I collagen is specific. The induction by collagen was mediated by α2β1 integrin receptor of cells. The osteoblastic differentiation was inhibited by the interruption of the binding of collagen with α2β1 integrin. Type I collagen contributes to the osteoblastic differentiation at the early stage. At the late stage, bone sialoprotein(BSP)had a crucial role for the differentiation. When the binding of BSP with receptor was suppressed by the presence of the specific antibody, the expression of osteoblastic phenotypes of bone marrow cells were suppressed. Type I collagen enhanced the formation of mineralized tissues by dental pulp cells in the medium containing β-glycerophosphate. These findings imply that extracellular matrix facilitates the formation of mineralized tissues.